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jeetered
Stranger



Registered: 07/07/06
Posts: 3,055
Loc: no clue
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Re: Jeetereds Easy Liquid Culture Tek *DELETED* [Re: nw_shroomy]
#7521071 - 10/15/07 09:15 PM (16 years, 3 months ago) |
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Post deleted by jeeteredReason for deletion: 9o
Edited by jeetered (10/15/07 10:06 PM)
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nw_shroomy
NoN-stranger


Registered: 01/02/06
Posts: 1,332
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Re: Jeetereds Easy Liquid Culture Tek [Re: jeetered]
#7521117 - 10/15/07 09:30 PM (16 years, 3 months ago) |
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I didnt think I was picking it apart.If so sorry.I was just wondering when to shoot the air in.Thanks for the reply and the write up.
-------------------- Spawn Ratio Calculator http://www.shroomery.org/forums/showflat.php/Number/7803673#7803673 I only grow edibles.Any info I give ONLY applies to gourmet mushrooms.
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jeetered
Stranger



Registered: 07/07/06
Posts: 3,055
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Re: Jeetereds Easy Liquid Culture Tek [Re: nw_shroomy]
#7521307 - 10/15/07 10:05 PM (16 years, 3 months ago) |
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Quote:
nw_shroomy said: I didnt think I was picking it apart.If so sorry.I was just wondering when to shoot the air in.Thanks for the reply and the write up.
My apologies nw_shroomy, I didn't mean to come off abrasive please do not be afraid to ask any questions. I guess I was a little rude. SOmetimes it's hard to tell when someones genuinely asking questions, and being rhetorical.
Please, feel free to ask whatever you wish. For some reason I got the wrong impression about the intentions of your questions.
For that I apologize.
Peace bro! and to others please don't be afraid to ask anything. I honestly didnt mean to come off like that.
Edited by jeetered (10/15/07 10:07 PM)
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Skeeblix
Dave Thomas



Registered: 01/28/07
Posts: 1,745
Loc: Wendy's
Last seen: 8 years, 3 months
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Re: Jeetereds Easy Liquid Culture Tek [Re: jeetered]
#7521512 - 10/15/07 10:40 PM (16 years, 3 months ago) |
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Good stuff as ever, buddy.
-------------------- This post approved by:
Premedman1 said:
I just shat my pants.
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Blutjager
Inhuman


Registered: 06/11/06
Posts: 9,220
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Re: Jeetereds Easy Liquid Culture Tek [Re: Skeeblix]
#7521559 - 10/15/07 10:50 PM (16 years, 3 months ago) |
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I like
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jeetered
Stranger



Registered: 07/07/06
Posts: 3,055
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Re: Jeetereds Easy Liquid Culture Tek [Re: Blutjager]
#7521670 - 10/15/07 11:27 PM (16 years, 3 months ago) |
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Thanks skeeblix and blutjager!
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blood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
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Re: Jeetereds Easy Liquid Culture Tek [Re: jeetered]
#7522378 - 10/16/07 09:20 AM (16 years, 3 months ago) |
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Quote:
jeetered said:
Quote:
blood4blood said: nice one jeetered. how many cc's of spore solution did you add to the medium?
less then .5 cc's of spore solution.
so you added less than half a cc and got results like that. thats badass. i was always told to use between 2-3 cc's but if i can get by with only half a cc thats awesome. like i said before great write up man very easy to follow
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jeetered
Stranger



Registered: 07/07/06
Posts: 3,055
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Re: Jeetereds Easy Liquid Culture Tek [Re: blood4blood]
#7522615 - 10/16/07 10:52 AM (16 years, 3 months ago) |
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GTHANKS
and yea, you only need about a half cc of spore solution to inoculate the LC.
LC 2 LC transfers about 2 cc's for explosive growth.
Clone flesh takes about 4 days.
agar wedges of isolate rhizo takes about 3 days.
Pans take about 2 days.
lolz.
peace
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knotty
n00b



Registered: 01/19/07
Posts: 126
Loc: DEE EFF DUB
Last seen: 14 years, 11 months
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Re: Jeetereds Easy Liquid Culture Tek [Re: jeetered]
#7576512 - 10/30/07 12:37 PM (16 years, 3 months ago) |
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sweet tek, ive tried lc a couple times, i dont have a pc YET so i tried to steam sterilze it both times. didnt work. yo jeetered..have an extra pc laying around anywhere
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Leonidas25
Stranger


Registered: 06/16/07
Posts: 85
Last seen: 15 years, 11 months
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Re: Jeetereds Easy Liquid Culture Tek [Re: roquet]
#7579975 - 10/31/07 09:50 AM (16 years, 3 months ago) |
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OK, guys tell me if this would work. Instead of using a syringe with polyfill for the air exchange(s). Can't I just open a new sterile syringe/needle and suck up some air from the laminar flow coming out of my flow hood and inject that. That way I can carefully recap the syringe with a new un-opened sterile needle tip, and use the same syringe in the same manner at a later date for other air exchanges?
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jeetered
Stranger



Registered: 07/07/06
Posts: 3,055
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Re: Jeetereds Easy Liquid Culture Tek [Re: Leonidas25]
#7580119 - 10/31/07 10:36 AM (16 years, 3 months ago) |
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Quote:
Leonidas25 said: OK, guys tell me if this would work. Instead of using a syringe with polyfill for the air exchange(s). Can't I just open a new sterile syringe/needle and suck up some air from the laminar flow coming out of my flow hood and inject that. That way I can carefully recap the syringe with a new un-opened sterile needle tip, and use the same syringe in the same manner at a later date for other air exchanges?
that would work just fine.
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Leonidas25
Stranger


Registered: 06/16/07
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Last seen: 15 years, 11 months
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Re: Jeetereds Easy Liquid Culture Tek [Re: jeetered]
#7589956 - 11/03/07 12:32 AM (16 years, 2 months ago) |
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I absolutely cannont find malt syrup anywhere locally...I already have the karo and everything else needed. Do you think 2 tbs of karo, in place of 1 tbs karo and 1 tbs malt, will be adequate for a cubensis strain?
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jeetered
Stranger



Registered: 07/07/06
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Re: Jeetereds Easy Liquid Culture Tek [Re: Leonidas25]
#7590288 - 11/03/07 06:39 AM (16 years, 2 months ago) |
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that would work just fine.
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Leonidas25
Stranger


Registered: 06/16/07
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Last seen: 15 years, 11 months
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Re: Jeetereds Easy Liquid Culture Tek [Re: jeetered]
#7590311 - 11/03/07 07:14 AM (16 years, 2 months ago) |
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Thanks Jeetered...this tek is awesome, really simple
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jeetered
Stranger



Registered: 07/07/06
Posts: 3,055
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Re: Jeetereds Easy Liquid Culture Tek [Re: Leonidas25]
#7590369 - 11/03/07 08:01 AM (16 years, 2 months ago) |
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you're welcome and thanks!
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QDP843
Entrepreneur


Registered: 03/05/07
Posts: 295
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Re: Jeetereds Easy Liquid Culture Tek [Re: jeetered]
#7641809 - 11/15/07 08:56 PM (16 years, 2 months ago) |
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thanks man, i will use this one day.
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Deception
HopefullyTripping Soon

Registered: 09/12/06
Posts: 88
Loc: Somewhere Over the Rainbo...
Last seen: 13 years, 2 months
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Re: Jeetereds Easy Liquid Culture Tek [Re: QDP843]
#7679188 - 11/25/07 06:12 PM (16 years, 2 months ago) |
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This is awesome timing man!! i've been wanting to start an lc for quite awhile because for some reason my spore syringes keep failing to get the job done.. i've seen no results on 4 innoc. in a row so i want to turn my spore print into a lc. this tek is the easiest one i've read yet and i have everything i need to do it. thanks for the newb friendly post any all the info
-------------------- If at first you don't succeed.. Hit it till your knuckles bleed..
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jeetered
Stranger



Registered: 07/07/06
Posts: 3,055
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Re: Jeetereds Easy Liquid Culture Tek [Re: Deception]
#7679256 - 11/25/07 06:34 PM (16 years, 2 months ago) |
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no problem!
good luck in your endeavors!
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