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Shop: Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds High THC Strains   North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures

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Invisiblejeetered
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Re: Jeetereds Easy Liquid Culture Tek *DELETED* [Re: nw_shroomy]
    #7521071 - 10/15/07 09:15 PM (16 years, 3 months ago)

Post deleted by jeetered

Reason for deletion: 9o



Edited by jeetered (10/15/07 10:06 PM)


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Invisiblenw_shroomy
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Re: Jeetereds Easy Liquid Culture Tek [Re: jeetered]
    #7521117 - 10/15/07 09:30 PM (16 years, 3 months ago)

I didnt think I was picking it apart.If so sorry.I was just wondering when to shoot the air in.Thanks for the reply and the write up.


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Spawn Ratio Calculator
http://www.shroomery.org/forums/showflat.php/Number/7803673#7803673
I only grow edibles.Any info I give ONLY applies to gourmet mushrooms.


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Invisiblejeetered
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Re: Jeetereds Easy Liquid Culture Tek [Re: nw_shroomy]
    #7521307 - 10/15/07 10:05 PM (16 years, 3 months ago)

Quote:

nw_shroomy said:
I didnt think I was picking it apart.If so sorry.I was just wondering when to shoot the air in.Thanks for the reply and the write up.




My apologies nw_shroomy, I didn't mean to come off abrasive please do not be afraid to ask any questions. I guess I was a little rude. SOmetimes it's hard to tell when someones genuinely asking questions, and being rhetorical.

Please, feel free to ask whatever you wish. For some reason I got the wrong impression about the intentions of your questions.

For that I apologize.

Peace bro! and to others please don't be afraid to ask anything. I honestly didnt mean to come off like that.


Edited by jeetered (10/15/07 10:07 PM)


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OfflineSkeeblix
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Re: Jeetereds Easy Liquid Culture Tek [Re: jeetered]
    #7521512 - 10/15/07 10:40 PM (16 years, 3 months ago)

Good stuff as ever, buddy.


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This post approved by:


Premedman1 said:
:lol: I just shat my pants.


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InvisibleBlutjager
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Re: Jeetereds Easy Liquid Culture Tek [Re: Skeeblix]
    #7521559 - 10/15/07 10:50 PM (16 years, 3 months ago)

I like:hehehe:


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Invisiblejeetered
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Re: Jeetereds Easy Liquid Culture Tek [Re: Blutjager]
    #7521670 - 10/15/07 11:27 PM (16 years, 3 months ago)

Thanks skeeblix and blutjager!


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Invisibleblood4blood
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Re: Jeetereds Easy Liquid Culture Tek [Re: jeetered]
    #7522378 - 10/16/07 09:20 AM (16 years, 3 months ago)

Quote:

jeetered said:
Quote:

blood4blood said:
nice one jeetered. how many cc's of spore solution did you add to the medium?




less then .5 cc's of spore solution.





so you added less than half a cc and got results like that. thats badass. i was always told to use between 2-3 cc's but if i can get by with only half a cc thats awesome. like i said before great write up man very easy to follow


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Invisiblejeetered
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Re: Jeetereds Easy Liquid Culture Tek [Re: blood4blood]
    #7522615 - 10/16/07 10:52 AM (16 years, 3 months ago)

GTHANKS

and yea, you only need about a half cc of spore solution to inoculate the LC.

LC 2 LC transfers about 2 cc's for explosive growth.

Clone flesh takes about 4 days.

agar wedges of isolate rhizo takes about 3 days.

Pans take about 2 days.

lolz.

peace


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Offlineknotty
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Re: Jeetereds Easy Liquid Culture Tek [Re: jeetered]
    #7576512 - 10/30/07 12:37 PM (16 years, 3 months ago)

sweet tek, ive tried lc a couple times, i dont have a pc YET so i tried to steam sterilze it both times. didnt work. yo jeetered..have an extra pc laying around anywhere :bigblunt:


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OfflineLeonidas25
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Re: Jeetereds Easy Liquid Culture Tek [Re: roquet]
    #7579975 - 10/31/07 09:50 AM (16 years, 3 months ago)

OK, guys tell me if this would work. Instead of using a syringe with polyfill for the air exchange(s). Can't I just open a new sterile syringe/needle and suck up some air from the laminar flow coming out of my flow hood and inject that. That way I can carefully recap the syringe with a new un-opened sterile needle tip, and use the same syringe in the same manner at a later date for other air exchanges?


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Invisiblejeetered
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Re: Jeetereds Easy Liquid Culture Tek [Re: Leonidas25]
    #7580119 - 10/31/07 10:36 AM (16 years, 3 months ago)

Quote:

Leonidas25 said:
OK, guys tell me if this would work. Instead of using a syringe with polyfill for the air exchange(s). Can't I just open a new sterile syringe/needle and suck up some air from the laminar flow coming out of my flow hood and inject that. That way I can carefully recap the syringe with a new un-opened sterile needle tip, and use the same syringe in the same manner at a later date for other air exchanges?




that would work just fine.


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OfflineLeonidas25
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Re: Jeetereds Easy Liquid Culture Tek [Re: jeetered]
    #7589956 - 11/03/07 12:32 AM (16 years, 2 months ago)

I absolutely cannont find malt syrup anywhere locally...I already have the karo and everything else needed. Do you think 2 tbs of karo, in place of 1 tbs karo and 1 tbs malt, will be adequate for a cubensis strain?


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Invisiblejeetered
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Re: Jeetereds Easy Liquid Culture Tek [Re: Leonidas25]
    #7590288 - 11/03/07 06:39 AM (16 years, 2 months ago)

that would work just fine.


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OfflineLeonidas25
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Re: Jeetereds Easy Liquid Culture Tek [Re: jeetered]
    #7590311 - 11/03/07 07:14 AM (16 years, 2 months ago)

Thanks Jeetered...this tek is awesome, really simple


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Invisiblejeetered
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Re: Jeetereds Easy Liquid Culture Tek [Re: Leonidas25]
    #7590369 - 11/03/07 08:01 AM (16 years, 2 months ago)

you're welcome and thanks!


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OfflineQDP843
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Re: Jeetereds Easy Liquid Culture Tek [Re: jeetered]
    #7641809 - 11/15/07 08:56 PM (16 years, 2 months ago)

thanks man, i will use this one day.


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OfflineDeception
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Re: Jeetereds Easy Liquid Culture Tek [Re: QDP843]
    #7679188 - 11/25/07 06:12 PM (16 years, 2 months ago)

This is awesome timing man!! :smile: i've been wanting to start an lc for quite awhile because for some reason my spore syringes keep failing to get the job done.. i've seen no results on 4 innoc. in a row so i want to turn my spore print into a lc. this tek is the easiest one i've read yet and i have everything i need to do it. thanks for the newb friendly post any all the info


--------------------
If at first you don't succeed..
Hit it till your knuckles bleed..


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Re: Jeetereds Easy Liquid Culture Tek [Re: Deception]
    #7679256 - 11/25/07 06:34 PM (16 years, 2 months ago)

no problem!

good luck in your endeavors!


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