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mikeytro
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Registered: 01/28/07
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grain to grain transfer...gone bad
#7516024 - 10/14/07 02:32 PM (16 years, 3 months ago) |
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so I did my first grain to grain transfer about a week and a half ago looking at the jars today about 2/3 of them have contams...which is a pretty bad failure ratio I plan on doing it again today on 12 jars
I've read a few teks on it and even used a glove box with latex gloves. however I did not use a mask or any sort of air filter. This time around I bought medical grade masks but a air filter isn't available.
any tips?
edit: just to clarify the glove box is a very make shift box that has no gloves attached..basically just holes to put hands in
-------------------- "Reality is merely an illusion, albeit a very persistent one." - Einstein
Edited by mikeytro (10/14/07 02:35 PM)
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Walter1496211
Window Washer



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Re: grain to grain transfer...gone bad [Re: mikeytro]
#7516035 - 10/14/07 02:35 PM (16 years, 3 months ago) |
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Sorry to hear that man.. I know this is probably way unorthodox.. But when I do G2G I pull everything out of my fridge which isn't much.. I clean the hell out of it I pull all the shelves out but one and I do it to it. I of course use gloves and surgical mask.. Anyway thats how I do it.
-------------------- you see the world through the window of your experience
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mikeytro
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Re: grain to grain transfer...gone bad [Re: Walter1496211]
#7516043 - 10/14/07 02:36 PM (16 years, 3 months ago) |
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why do it in the fridge? dont you have to keep the door open anyways? am I missing something? anyways thanks for the idea nonetheless
-------------------- "Reality is merely an illusion, albeit a very persistent one." - Einstein
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simplemachine
Manfly


Registered: 09/14/03
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Re: grain to grain transfer...gone bad [Re: mikeytro]
#7516045 - 10/14/07 02:37 PM (16 years, 3 months ago) |
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Using a still air box should be plenty.
Maybe the master jar was hiding some contams?
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mikeytro
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Re: grain to grain transfer...gone bad [Re: simplemachine]
#7516053 - 10/14/07 02:39 PM (16 years, 3 months ago) |
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hmmm maybe you're right I actually used 3 half pint jars in the first transfer which may actually give reason to why some are contaminated and others arnt we'll see how round 2 goes..
-------------------- "Reality is merely an illusion, albeit a very persistent one." - Einstein
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Walter1496211
Window Washer



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Re: grain to grain transfer...gone bad [Re: simplemachine]
#7516056 - 10/14/07 02:40 PM (16 years, 3 months ago) |
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Yea I keep it open... Like I said not a practice that many would condone but my reasoning is that I don't think bacteria and mold are really airborne at lower temperatures so I figure there is less of a chance. I could be wrong but its always worked for me.
-------------------- you see the world through the window of your experience
Edited by Walter1496211 (10/14/07 02:43 PM)
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mikeytro
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Re: grain to grain transfer...gone bad [Re: Walter1496211]
#7516057 - 10/14/07 02:40 PM (16 years, 3 months ago) |
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ahhh I see, because the drop in temperature interesting method
-------------------- "Reality is merely an illusion, albeit a very persistent one." - Einstein
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Walter1496211
Window Washer



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Re: grain to grain transfer...gone bad [Re: mikeytro]
#7516066 - 10/14/07 02:43 PM (16 years, 3 months ago) |
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I have never really heard of anyone else doing it my way so I can't say its full proof.
-------------------- you see the world through the window of your experience
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simplemachine
Manfly


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Re: grain to grain transfer...gone bad [Re: mikeytro]
#7516087 - 10/14/07 02:47 PM (16 years, 3 months ago) |
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Quote:
mikeytro said: hmmm maybe you're right I actually used 3 half pint jars in the first transfer which may actually give reason to why some are contaminated and others arnt we'll see how round 2 goes..
Thats what it sounds like then...Plus its the only reason I've had failure rates that high.
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acommunistspy
the fun nazi,NOT to beconfused withthe fun-nazi



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Re: grain to grain transfer...gone bad *DELETED* [Re: Walter1496211]
#7516097 - 10/14/07 02:49 PM (16 years, 3 months ago) |
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Post deleted by acommunistspyReason for deletion: this never happened
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Edited by acommunistspy (10/14/07 02:52 PM)
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Pr0_X
CultivationLifer



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Re: grain to grain transfer...gone bad [Re: acommunistspy]
#7516181 - 10/14/07 03:15 PM (16 years, 3 months ago) |
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I never had much luck with G2G's even when done in a glovebox, G2G is inferior to LC anyways.
Best luck to you and hopefully we see a some pic's of a tier filled with myco pr0n soon
-------------------- It's okay to hurt my feelings cause you know, they're so numb anyway. but I guess it's what I get for being to fuckin stupid to stay away - Jake - Support the FSR at www.fsrcanada.com and www.fsre.nl
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RogerRabbit
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Re: grain to grain transfer...gone bad [Re: Pr0_X]
#7516380 - 10/14/07 04:03 PM (16 years, 3 months ago) |
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Quote:
G2G is inferior to LC anyways.
Not correct. I have at least a 99.99% success rate with grain to grain transfers. The key is to learn to do it right. If you don't have a flow hood, you'll need 100% still air. Have the jar lids off no more than five seconds. Don't set the lid down. Hold it with one hand while pouring from your master jar with the other hand. This allows you to return the lid as soon as possible. Don't stick anything into the master. Bang it against a tire to break it up, and then pour the grains out like sand.
Clean your glovebox and leave damp(with water). Wash your gloves with alcohol after putting them on. Wipe the jars and lids with an alcohol soaked towel prior to opening. Work fast and accurately. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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mikeytro
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Re: grain to grain transfer...gone bad [Re: RogerRabbit]
#7516495 - 10/14/07 04:27 PM (16 years, 3 months ago) |
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RR thanks for the advise on not setting the lid down + holding it, did that last time
-------------------- "Reality is merely an illusion, albeit a very persistent one." - Einstein
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The shroomy 1
Luminous beings surround me




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Re: grain to grain transfer...gone bad [Re: mikeytro]
#7516986 - 10/14/07 06:37 PM (16 years, 3 months ago) |
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Quote:
mikeytro said: hmmm maybe you're right I actually used 3 half pint jars in the first transfer which may actually give reason to why some are contaminated and others arnt we'll see how round 2 goes..
I would like to recommend that you use larger jars. Try quart jars instead. If indeed it was the master jar that was contaminated, you would have seen it in the quart jar long before you would have thought of doing the transfer.
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AMU Q&A thread.
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mikeytro
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Re: grain to grain transfer...gone bad [Re: The shroomy 1]
#7517119 - 10/14/07 07:08 PM (16 years, 3 months ago) |
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yea I use 1/2 pints, pints and quart jars just had em all laying around so I thought why not
-------------------- "Reality is merely an illusion, albeit a very persistent one." - Einstein
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The shroomy 1
Luminous beings surround me




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Re: grain to grain transfer...gone bad [Re: mikeytro]
#7517403 - 10/14/07 08:41 PM (16 years, 3 months ago) |
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The only use I have for 1/2 pints now is for print making and using them as Petri Dishes. You should really concentrate on larger jars. In my opinion.
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AMU Q&A thread.
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mikeytro
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Re: grain to grain transfer...gone bad [Re: The shroomy 1]
#7517509 - 10/14/07 09:14 PM (16 years, 3 months ago) |
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I have 12 quarts 18 pints and 8 half pints alright I know its wiser to do larger jars but who really cares..wanted in knock up as many as possible
-------------------- "Reality is merely an illusion, albeit a very persistent one." - Einstein
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The shroomy 1
Luminous beings surround me




Registered: 03/27/07
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Re: grain to grain transfer...gone bad [Re: mikeytro]
#7517557 - 10/14/07 09:27 PM (16 years, 3 months ago) |
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Quote:
mikeytro said: alright I know its wiser to do larger jars but who really cares.
Holy crap! I was under the impression that YOU DID!
Quote:
so I did my first grain to grain transfer about a week and a half ago looking at the jars today about 2/3 of them have contams...which is a pretty bad failure ratio
With those contamination ratios I'd be listening to advice.
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AMU Q&A thread.
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anarchOi
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Re: grain to grain transfer...gone bad [Re: The shroomy 1]
#7517590 - 10/14/07 09:37 PM (16 years, 3 months ago) |
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my first glovebox was a failure, i tried to make it with kitchen gloves but i did it wrong and the gloves sucked so i'll have to try making another
so far, open air has worked pretty well for me, i use a larger room (preferably not the kitchen) and Oust like mad, as long as you don't have airflow, it's pretty hard for contams to get in unless you've been waving trich/cobweb infections everywhere in your area xD
if you're using a smaller room, say a super-cleaned bathroom, wouldn't you need to stay inside of it after oust'n like crazy? if not, when you open the door you're letting all your cleaned air out and "fresh air" in
it's likely a contam in your grain that made it through your sterilization process, as you said.
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mikeytro
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Re: grain to grain transfer...gone bad [Re: The shroomy 1]
#7517955 - 10/14/07 11:02 PM (16 years, 3 months ago) |
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what does jar size have to do with failure ratio? by wiser I actually ment more logical...but hey why would I not inoculate as many as possible that I have on hand? and finally please stop acting like a douche
-------------------- "Reality is merely an illusion, albeit a very persistent one." - Einstein
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