Home | Community | Message Board

MushroomCube.com
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract

Jump to first unread post Pages: 1 | 2 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineCheesekiller
Mad Scientist
Male


Registered: 11/08/04
Posts: 485
Loc: Central NY
Last seen: 2 months, 29 days
First LC
    #7515159 - 10/14/07 09:46 AM (16 years, 5 months ago)

My friend gave me some syringes. One was a Koh Samui super strain. Problem was that one only had about 2 CC's left. So I decided to make a LC. I used about two tablespoons of light karo corn syrup, in about 12 oz of water. I used one of the same lids from my grain jars.



PC for 20 min @15PSI. Flame sterilize the needle and knocked up the cool jar. I have had it incubating at 80F for about 5 days now.




Questions.
1)Is it all done.

2) when I use it to knock up my grain jars do I have to make sure and get a bunch of that cloud in my syringe?

3) Is it bad to shake it up at this time?


--------------------
Bulk grower with "some" success.

  Cloning Machine 
   
Nice Lids

A few pics of my DT setup

Extras: Filter Print Post Top
OfflineXeluc
Traveler
Male


Registered: 04/11/07
Posts: 746
Last seen: 6 years, 2 months
Re: First LC [Re: Cheesekiller]
    #7515356 - 10/14/07 11:08 AM (16 years, 5 months ago)

1) Looks good to me.
2) Sure, although if there's any VISIBLE mycelium in the syringe you use, you have plenty, I wouldn't worry too much about it
3)nope! I would, although like #2, it doesn't really matter as long as you end up with myc in the syringe, it wont hurt it to shake though.

Extras: Filter Print Post Top
OfflineCheesekiller
Mad Scientist
Male


Registered: 11/08/04
Posts: 485
Loc: Central NY
Last seen: 2 months, 29 days
Re: First LC [Re: Xeluc]
    #7515413 - 10/14/07 11:27 AM (16 years, 5 months ago)

Thanks, I have heard that this Koh Samui is explosive, so this LC should really help out the colonization.

Any tips on getting the stuff into the syringe? I was thinking about using alcohol to sterilize a few empty syringes, maybe fill and drain once or twice?? Then fill up the syringe with the LC? I'm not too sure about that? I could PC the syringes with water, then drain when cold and fill like that??

After that I have the process down pretty good.

-CK


--------------------
Bulk grower with "some" success.

  Cloning Machine 
   
Nice Lids

A few pics of my DT setup

Extras: Filter Print Post Top
OfflineXeluc
Traveler
Male


Registered: 04/11/07
Posts: 746
Last seen: 6 years, 2 months
Re: First LC [Re: Cheesekiller]
    #7515699 - 10/14/07 12:51 PM (16 years, 5 months ago)

cover syringes in foil, boil or pc them, then load up. :smile: the syringes don't have to be filled when sterilizing, i wouldn't even fill them. As for how to suck it up, just stick your needle in and aim for the cloud of myc! If you have a hard time sucking it up, shake shake shake!

Extras: Filter Print Post Top
OfflineFunkatron9000
Astronaut
Male User Gallery


Registered: 10/03/07
Posts: 535
Loc: Sector Zed: Region 6: Sol...
Last seen: 2 years, 4 months
Re: First LC [Re: Xeluc]
    #7515835 - 10/14/07 01:29 PM (16 years, 5 months ago)

looking good man, boil some water up and suck it in the syringe acouple times. let it cool and get in there and show that cloud who is boss.


--------------------
You cannot yet touch my daughters awesome boobage
:jiggly:
You must fight to the death in the Breastriary of Nippopolis.




I thought Gene Wilder was cool BEFORE he was dead.

Extras: Filter Print Post Top
OfflineCheesekiller
Mad Scientist
Male


Registered: 11/08/04
Posts: 485
Loc: Central NY
Last seen: 2 months, 29 days
Re: First LC [Re: Funkatron9000]
    #7516574 - 10/14/07 04:46 PM (16 years, 5 months ago)

Sweet, I'll post pics of this periodically.

-CK


--------------------
Bulk grower with "some" success.

  Cloning Machine 
   
Nice Lids

A few pics of my DT setup

Extras: Filter Print Post Top
Offlinejust me
Friend
Male User Gallery


Registered: 07/01/07
Posts: 9,745
Loc: IL/MO/FL/TX/HI/OR
Last seen: 4 years, 9 months
Re: First LC [Re: Cheesekiller]
    #7516625 - 10/14/07 04:59 PM (16 years, 5 months ago)

in the same boat as you killer, my LC doesnt look as pretty as yours tho. hope you dont mind if i post a pic of it on here, but i have all the same Q's as you, and just need someone to tell me "its ok" haha

thanks for starting this thread tho, all my Q's other than is my LC ok were answered!



thanks, just me


--------------------

--------------------------------------------------

-pEaCeLoVeGoDbLeSs-

"The Downfall of Mankind; is Believing He Has Limitations."

Extras: Filter Print Post Top
Invisiblemushbaby
woodswalker
Female User Gallery


Folding@home Statistics
Registered: 09/30/06
Posts: 2,645
Loc: in my own lil world
Re: First LC [Re: just me]
    #7517219 - 10/14/07 07:39 PM (16 years, 5 months ago)

Y'all meant stir not shake correct? Try not to get the filter patch wet. Possible way for contaminants to be introduced to your lc. But it might depend on the filter patch.


--------------------

Extras: Filter Print Post Top
OfflineXeluc
Traveler
Male


Registered: 04/11/07
Posts: 746
Last seen: 6 years, 2 months
Re: First LC [Re: mushbaby]
    #7517327 - 10/14/07 08:21 PM (16 years, 5 months ago)

well when i said shake, I was thinking he had a tyvek filter, which repels water (unless the lc is real hot for some reason, then I get small droplets forming) regardless though, "Agitate" your LC :wink:

Extras: Filter Print Post Top
Invisiblelarge_dose
Melonhead
 User Gallery


Registered: 05/05/06
Posts: 1,346
Loc: Right in the Middle
Trusted Cultivator
Re: First LC [Re: Xeluc]
    #7517419 - 10/14/07 08:46 PM (16 years, 5 months ago)

From what it looks like you could get at least 3-4 loaded syringes.

I usually aim right for the cloud and try to suck up as much as myc possible. Once im done there is visible myc all the way up the syringe.

I never shake at that stage.

Heres a pic of Karo LC's done the exact procedure as yours 3 weeks in:


If your's have only been colonizing 5 days than thats some aggressive stuff


Looks great to me.:thumbup:

Extras: Filter Print Post Top
OfflineCheesekiller
Mad Scientist
Male


Registered: 11/08/04
Posts: 485
Loc: Central NY
Last seen: 2 months, 29 days
Re: First LC [Re: large_dose]
    #7528731 - 10/17/07 05:50 PM (16 years, 5 months ago)

Getting the stuff into the syringe was tough. It snotted up when trying to aspirate, so I shook. Then I got floaties in the syringes. I blasted 7 QTs WBS with the stuff yesterday, and threw them in the incubator. There is a little activity today but nothing to post pics of yet.

Next time I'm gonna use a real low pro jar for the LC, so I can get my syringe in there a little easier (without having to tilt it on its side).

Wish you all luck. I'm betting 6 days to full colonization. I have to get my poo ready soon!! Any bets??

-CK


--------------------
Bulk grower with "some" success.

  Cloning Machine 
   
Nice Lids

A few pics of my DT setup

Extras: Filter Print Post Top
OfflineMepher
Reverend
Male User Gallery

Registered: 11/18/05
Posts: 129
Loc: Praha, CZK
Last seen: 2 years, 4 months
Re: First LC [Re: Cheesekiller]
    #7529861 - 10/18/07 01:34 AM (16 years, 5 months ago)

Looks like you're on the right path, Cheesekiller. It can take a few days to see growth, but your jars will go WAY faster with the LC than using ungerminated spores. Good luck!

Extras: Filter Print Post Top
OfflineCheesekiller
Mad Scientist
Male


Registered: 11/08/04
Posts: 485
Loc: Central NY
Last seen: 2 months, 29 days
CONTAMINATED [Re: Mepher]
    #7552178 - 10/23/07 06:40 PM (16 years, 5 months ago)

:frown:

I think when I shook up the LC I got contams somehow.  All of the 7 qt jars had white shit all over them after 2 days.  The I noticed green SHIT.

Another error that may have caused (probably) was I forgot to shake the jars after PC'ing.  So the next day I sterilized a knife, broke the jars open, and agitated with the knife.  This could have sucked in contams. 

This worked for me in the past when I forgot that crucial step (shaking after PCing) with no contamination, so the only other source  would have been the original syringe. I got it with only 2CC's of left over from a friend. 

Question. 

Wouldn't I have noticed contams in the LC??  It looked like the lid had some crap on the inside of the tyvek patch.  I figured from shaking it up. 

Does the LC actually need air exchange?

With that I just PC'ed 7 more qts with WBS, and have some fresh home made muli-spore Amazonian Cube syringes.  Never fails.  This is not the last attempt at an LC however.

-CK


--------------------
Bulk grower with "some" success.

  Cloning Machine 
   
Nice Lids

A few pics of my DT setup

Extras: Filter Print Post Top
OfflineVisionsToReality
RIBBONS


Registered: 09/22/07
Posts: 1,083
Last seen: 16 years, 3 months
Re: CONTAMINATED [Re: Cheesekiller]
    #7552269 - 10/23/07 06:59 PM (16 years, 5 months ago)

Ive read LCs do better with air exchange but its not essential
and that contams are hard to notice in LC until you inoc grains and then it becomes evident (a downside of LC, whereas w/ g2g this is not the case)


--------------------
Life is one big road with lots of signs,
So when you're ridin' through the ruts,
Don't you complicate your mind.

Flee from hate, mischief and jealousy
Don't bury your thoughts,
Put your vision to reality, yeah!

Extras: Filter Print Post Top
Invisibledjsollest
workin hard
Male User Gallery


Registered: 08/25/07
Posts: 121
Re: CONTAMINATED [Re: Cheesekiller]
    #7552293 - 10/23/07 07:04 PM (16 years, 5 months ago)

Hey man all my LC's have turned out JUST like yours and I had the exact same thing happen in my brf jars after inoc with the LC . They all had nice white growth in a day or two then like day 3-4 I saw little green dots EVERYWHERE!?!? Whats the deal with this? And what do you mean "shaking after PCing jars"
Ive never heard of this, could you explain it?


--------------------
"DRUM N BASS IN YER FACE"
"a love to sleep with opium dreams"

My first grow! Brazilians with H poo / Coir
http://www.shroomery.org/forums/showflat.php/Number/7730494#7730494

Extras: Filter Print Post Top
Offlinecroptop30
Stranger
Registered: 09/27/07
Posts: 37
Last seen: 16 years, 4 months
Re: CONTAMINATED [Re: djsollest]
    #7552368 - 10/23/07 07:20 PM (16 years, 5 months ago)

I never use an air hole I use baby bottles with RTV sealant in the nipple and I throw a marble in so you can stir it. you don't need alot of fresh air it will grow without an air hole. let it grow in a dark closet for about a week or two and then throw it in the frigerator from then out it will stay fresh for along time.

Extras: Filter Print Post Top
Offlineprimordialoddity
Hooded ForestDweller


Registered: 08/15/07
Posts: 182
Loc: PNW
Last seen: 14 years, 3 months
Re: CONTAMINATED [Re: croptop30]
    #7553257 - 10/23/07 11:13 PM (16 years, 5 months ago)

I shot up 2 quarts with 2-3ml of B+ spores in 500ml malt/dex...

it has been 2.5 days and I see no growth, good or bad... Thought i'd have visible myc by now, but I'll wait a week then try again...

I made my own stirrer...I;m itchn to use it!


--------------------
...to unite the powers of the conscious and unconscious, of intelligence and emotion.

NOTE: All posts and/or messages from this account are 100% fictional and should not be taken literally.

Extras: Filter Print Post Top
Offlineveda_sticks
Cultivator
Male User Gallery


Folding@home Statistics
Registered: 07/29/07
Posts: 14,191
Loc: UK Flag
Last seen: 4 years, 2 months
Re: CONTAMINATED [Re: primordialoddity]
    #7553682 - 10/24/07 04:31 AM (16 years, 5 months ago)

its takes 3-4 days for the spores to germinate, wait longer.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

Extras: Filter Print Post Top
OfflineCheesekiller
Mad Scientist
Male


Registered: 11/08/04
Posts: 485
Loc: Central NY
Last seen: 2 months, 29 days
Re: CONTAMINATED [Re: djsollest]
    #7554364 - 10/24/07 10:17 AM (16 years, 5 months ago)

Quote:

djsollest said:
And what do you mean "shaking after PCing jars"
Ive never heard of this, could you explain it?




This is because if you are using grains like WBS in particular, the grains get all clotted together after pressure cooking. If you shake them about an hour after PCing then the grains break up easily. In this case I had forgotten, then used a sterilized knife to break them up. I really think my contamination came from the LC though. Next time I will try Agars tek with the baby bottles etc.

-CK


--------------------
Bulk grower with "some" success.

  Cloning Machine 
   
Nice Lids

A few pics of my DT setup

Extras: Filter Print Post Top
OfflineMotorCityMadman
Rubberfan andFunketeer
 User Gallery


Registered: 10/02/06
Posts: 202
Loc: Motown (State of Mind)
Last seen: 14 years, 4 months
Re: CONTAMINATED [Re: Cheesekiller]
    #7555068 - 10/24/07 01:53 PM (16 years, 5 months ago)

Personally I've had way more contams with LCs than anything else. I'm strictly g2g at this point. Only reason I would ever do LC is if I were trying to knock more jars than would fit in my glove box.

Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | Next >  [ show all ]

Shop: Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract


Similar ThreadsPosterViewsRepliesLast post
* PCing with polyfill jars DoDo 3,468 15 01/19/03 05:49 AM
by debianlinux
* Is PCing an LC Kalix 485 5 06/26/05 12:23 AM
by deanofmean
* vaccume seal after PCing? MsPacMan 905 4 10/08/02 02:00 AM
by Magash
* How Many People Love Malt and Dextrose LC's but
( 1 2 3 all )
HippieChick 5,758 41 06/20/05 12:45 PM
by Roadkill
* I am scared to do a Print/syring!
( 1 2 all )
fearforce7 9,081 27 12/30/21 08:54 AM
by Forrester
* Newbie about to dive in head first - amount of spore syring CCs into 4 lb grow bag?
( 1 2 all )
ChrisHippyLoser 15,007 25 06/02/16 05:47 PM
by OldSawbonesHisself
* anyone ever try sterilizing a syringe without PCing? Gr0wer 841 10 09/28/04 04:56 PM
by Gr0wer
* Many sub-strains in a multispore LC? Similar to different types of myc on an agar plate? blackout 1,766 5 07/06/05 12:22 PM
by Roadkill

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
3,004 topic views. 47 members, 266 guests and 102 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.029 seconds spending 0.01 seconds on 16 queries.