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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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Invisible2012shaman
Stranger

Registered: 08/22/07
Posts: 338
Sources of contamination
    #7513849 - 10/14/07 12:21 AM (13 years, 6 months ago)

I have the worst luck with contams and I know its something im doing wrong during inoculation, every time I can see mold on the second day down the side of the jar where I inoculated, so I have decided to start a list of every possible source of contamination during every step of the cultivation process.OK ill start:
Dirty hair
Dirty clothes
Not flaming the needle
Breathing on your work
area during inoculation
These are just a few so I encourage every one to add to the list.Thank you.


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Invisiblemonstermitch
Growing in Bags Doesn't Work


Folding@home Statistics
Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay Flag
Re: Sources of contamination [Re: 2012shaman]
    #7513858 - 10/14/07 12:24 AM (13 years, 6 months ago)

you're listing off sources of bacterial contamination mostly.
your problem is with mold.

and by your description, it sounds as though there is mold
in your inoculant solution.
try a different inoculant.


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OfflinePsilocin Dreams
PerpetuallyContaminated


Registered: 08/03/07
Posts: 187
Last seen: 2 years, 10 months
Re: Sources of contamination [Re: 2012shaman]
    #7513886 - 10/14/07 12:30 AM (13 years, 6 months ago)

The one thing you don't have control over is whether or not the solution within the syringe itself is contaminated before you receive it. I believe this is what may have happened to me the first time. I added a few extra security steps on my second attempt, and all the jars came out fine. Here are the steps I took, perhaps they can help you as well.

1. Bake the substrate materials separately for 20 minutes at 300 degrees (don't forget to put extra verm in there for the top layer)
2. Boil the water that you plan to use in the substrate for 15 minutes
3. Use a Ramsey Seal
4. Inoculate on the edge of the oven (hot air rises and brings the contams up with it, which keeps them from falling down onto the substrate)
5. Use one of those paper masks that goes over your mouth/nose

Good luck in the future my brother


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Invisible2012shaman
Stranger

Registered: 08/22/07
Posts: 338
Re: Sources of contamination [Re: monstermitch]
    #7513890 - 10/14/07 12:31 AM (13 years, 6 months ago)

Do you mean a contaminated syringe?


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Invisiblemonstermitch
Growing in Bags Doesn't Work


Folding@home Statistics
Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay Flag
Re: Sources of contamination [Re: Psilocin Dreams]
    #7513917 - 10/14/07 12:36 AM (13 years, 6 months ago)

Quote:

Psilocin Dreams said:
4. Inoculate on the edge of the oven (hot air rises and brings the contams up with it, which keeps them from falling down onto the substrate)
5. Use one of those paper masks that goes over your mouth/nose

Good luck in the future my brother




please do NOT follow this advice:
Read Me
and me...

inoculating in an oven is a bad idea.
using a paper mask is a bad idea.

Quote:

2012shaman said:
Do you mean a contaminated syringe?



possibly.


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Offlinetheratatat
Hobbyist
 User Gallery


Registered: 03/09/07
Posts: 209
Last seen: 8 years, 8 months
Re: Sources of contamination [Re: Psilocin Dreams]
    #7514213 - 10/14/07 02:03 AM (13 years, 6 months ago)

Why not just save all that trouble and build a 10dollar glovebox when knocking up your jars?

Cheers


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