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OfflineToodles
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Using mycelium to colonize jars.
    #7512913 - 10/13/07 05:53 PM (16 years, 3 months ago)

just a quick question. would it be possible to crack up a brf cake into small pieces and then put each piece in a separate jar with wbs? just trying to think of ways to cut down the time from spore to mycelium and just have active mycelium colonize a substrate.


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OfflineD4NK
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Re: Using mycelium to colonize jars. [Re: Toodles]
    #7512929 - 10/13/07 05:58 PM (16 years, 3 months ago)

Isn't that just the same concept as a grain to grain transfer?  Except it's cake to grain?  Seems pretty fool proof to me as long as the cake is sterile, as well as the WBS.

:thumbup:


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OfflineThe_Bomb
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Re: Using mycelium to colonize jars. [Re: D4NK]
    #7513045 - 10/13/07 06:21 PM (16 years, 3 months ago)

I would think that this would work. Actually a few days ago i set up an experiment involving taking a piece of mycelium and dropping it in a BRF/Verm mix, Only thing i have to worry about is contams (and i think there's to much water in the jar). Other than that there is no reason it wouldnt work i dont think.


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OfflineWalter1496211
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Re: Using mycelium to colonize jars. [Re: The_Bomb]
    #7513048 - 10/13/07 06:23 PM (16 years, 3 months ago)

Of course but contam risk is high especially with WBS.


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OfflineRogerRabbitM
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Re: Using mycelium to colonize jars. [Re: Walter1496211]
    #7513393 - 10/13/07 08:15 PM (16 years, 3 months ago)

You would have less chance of contamination if you injected some water deep into a fully colonized cake, and then drew it back out again. Use this as your inoculant for the wbs.
RR


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OfflineWalter1496211
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Re: Using mycelium to colonize jars. [Re: RogerRabbit]
    #7513475 - 10/13/07 08:42 PM (16 years, 3 months ago)

That is excellent advice I think I will do that instead of G2G.


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Offlineboomer q
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Re: Using mycelium to colonize jars. [Re: Walter1496211]
    #7513959 - 10/13/07 10:46 PM (16 years, 3 months ago)

indeed, im gonna try that soon!


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