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Offlinemike3000
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Registered: 09/05/07
Posts: 106
Last seen: 3 years, 7 months
Preventing Contamination -Nooby question-
    #7500531 - 10/09/07 05:46 AM (16 years, 3 months ago)

I was just thinking about this and I used the search function and nothing relevant showed up.

What I was wondering was if you were to incubate mycelium at a lower temperature (I know it would colonize slower) But would it possibly prevent some contams from growing due to the cooler temperatures?
This is if hypothetically the inoculation was not fully sterile.


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If we are here to figure out the meaning of life,
then isn't the meaning of life to find out why we are here?
Causing our existence to be pointless!


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Offlineveda_sticks
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Re: Preventing Contamination -Nooby question- [Re: mike3000]
    #7500555 - 10/09/07 05:58 AM (16 years, 3 months ago)

Nothing will replace being sterile enough to not let contams in a jar. yes contams would grow slower but they still grow much faster than mycelium, i have tried a few times by moving a jar that showed a contam to a cooler place to end up with a big mess of mould and dying mycelium.

Do not skimp on sterile procedures, it just sets you back several days to weeks in your grow. If ur lucky it will show itself in the first few days, but sometimes it may not show for a week or 2 weeks.

Just be sterile, remember we are growing mushrooms in an environment that not only favours the growth of mushroom mycelium but many other moulds and bacteria.

U need to give your mycelium the best possible chance of growing so that when its time to birth they should be fairly resistant to contams.

Its not that difficult to be sterile, use a still air box in a small room with no drafts etc that has been disinfected, and disinfect your working materials in the glovebox then carrie out your procedures and u should only see a contam every o nce in a while.


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Invisiblemycocurious
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Registered: 02/09/07
Posts: 1,265
Re: Preventing Contamination -Nooby question- [Re: mike3000]
    #7501899 - 10/09/07 03:29 PM (16 years, 3 months ago)

Quote:

mike3000 said:What I was wondering was if you were to incubate mycelium at a lower temperature (I know it would colonize slower) But would it possibly prevent some contams from growing due to the cooler temperatures?




It's a little hard to give a definitive answer because it's technically a trick question.

Will incubating a cooler temperatures prevent contaminations? No.
Will incubating a higher temperatures cause more contaminations? Yes.

---
The reason lies in the differences between _ideal_ temps for incubation of your mycelia versus that of a bacterial or fungal contaminate.  While warmer is better for all parties involved - up to about 80(F) then it starts to actually have diminishing returns for are targeted mycelium - sometimes keeping your jars on the cooler side can help you side-step a contamination.

How?

Because our mycelium colonizes admirably in the low 70(F)'s whereas most bacterial and several other fungal contaminates lay dormant.  And, if you can manage to keep those contaminates dormant until your favored mycelium can consolidate control over the entire substrate as a whole, it will isolate and in some cases even kill the contaminate spores/endospores before they get a chance to germinate at all through it's natural secretion of metabolites**.

So like I said, it's more of a trick question than anything because what you're really asking is the corollary to the question "is it bad to incubate at higher temperatures?"  Which is yes, it is.

(grammar nazis - did I use corollary in right context?) :grin:

** - metabolites are the natural secretion of mycelium and is not specifically related to the presence of any contaminate, however, the metabolites themselves do have antiseptic qualities.


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:justdontknow: Don't mistake my tone for a "matter-of-fact" attitude.  I'm just presenting what I believe to be correct, until I'm corrected...

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figgusfiddus said:
Keep in mind that inoculating or whatever in front of a flow hood won't help your bad substrate, your bad inoculant, your bad sterile procedure, etc. etc. etc. It's not a +3 flowhood of magic, it's just a tool.


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OfflineAgave
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Registered: 08/29/07
Posts: 126
Last seen: 15 years, 9 months
Re: Preventing Contamination -Nooby question- [Re: mycocurious]
    #7504001 - 10/10/07 01:10 AM (16 years, 3 months ago)

I wouldn't put much faith in that keeping your cakes/casing contamination free...


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