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OfflineCyber
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Cloning questions
    #7421668 - 09/17/07 10:10 PM (16 years, 6 months ago)

I have had an issue with one strain. First off, it tends to produce sterile and mutant fruit. Not that I am complaining, the mutation tends to have some neat traits. Such as stalks that are 6" in diameter, 7" long, solid, with a cap that is about 6.5" in diameter. The also are STRONG! They blue with in moments of the slightest touch. So to start, if I clone from the mutant, will all the next batch be mutants, and if I clone from a good looking clump of fruit that produce good sporealation (is that a word?) and I clone from the fruit body, will the fruit from the next batch not have the mutant issue?

I guess I am asking can I select traits via cloning for propagation?

I remember reading some where that continued cloning of the fruit will produce mushrooms that no longer drop spores. Is this the case or is that an old wife's tale?

Is there a right and wrong way to clone?

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OfflineRogerRabbitM
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Re: Cloning questions [Re: Cyber]
    #7422553 - 09/18/07 06:50 AM (16 years, 6 months ago)

Normally, the traits in a fruit will carry over if you clone it. If you clone a mutant, you'll get harvests of mutants, provided the mutation was genetic in nature. If you clone from a cluster of large fruits, your future crops will develop clusters of large fruits.

Senescence is always a problem if you try to keep a fungal cell line going for too long. It isn't necessary though. Instead of constantly cloning the clones, clone the first one and then make a few master culture slants in test tubes to store the mycelium. You can then take it out of the refrigerator and use it for years to come without degradation.
RR


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OfflineCyber
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Re: Cloning questions [Re: RogerRabbit]
    #7440960 - 09/22/07 04:14 PM (16 years, 6 months ago)

Quote:

provided the mutation was genetic in nature.




What environmental factors could or would cause mutations in the fruit?

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OfflineRogerRabbitM
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Re: Cloning questions [Re: Cyber]
    #7441405 - 09/22/07 06:24 PM (16 years, 6 months ago)

Damage as the pins are forming, or for another example, damage from chemicals containing petroleum can be mistaken for mutations. I doubt there's an environmental condition you can easily create that would cause mutations.
RR


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InvisibleCaptain Cubensis
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Registered: 09/18/07
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Re: Cloning questions [Re: RogerRabbit]
    #7485316 - 10/04/07 03:00 PM (16 years, 5 months ago)

I found that using too strong a peroxide mix causes mutations like upside down caps with gills on the top.


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OfflineFunkatron9000
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Re: Cloning questions [Re: Captain Cubensis]
    #7485624 - 10/04/07 04:49 PM (16 years, 5 months ago)

lol, i'd like to see a upside down cap sometime. mabye i'll try a small batch and see if I can produce that sometime


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InvisibleCaptain Cubensis
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Registered: 09/18/07
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Re: Cloning questions [Re: Funkatron9000]
    #7492633 - 10/06/07 08:48 PM (16 years, 5 months ago)

Well, they were more like gills top and bottom, not really upside down, but with no smooth surface, all gills!


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Get 350lbs shipped to your door for only $100!
www.mycosupply.com
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Offlinerodfarva
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Re: Cloning questions [Re: Captain Cubensis]
    #7499031 - 10/08/07 07:15 PM (16 years, 5 months ago)

Cyber, im in the D.. keep that in mind if u ever need to compair notes. (need suplies or trade possibly?)

At any rate im about to do my first attempt at cloning within a day or two. I already have two good LC's that have worked amazaing from a BRF cake biopsy (pixs) Im sure there is something i haven't thought of, still i just want to clone to have an easy reproductive source beyone the PITA of spore growth.

with that said, what i did was pop a needle into a BRF cake and inject the biopsy into my LC media. Is this technically a clone? I dont know. I have a master and a 2nd generation Lc (clone?) however they both are in baby bottles. Is there a reason you recoment test tubes rodger?


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