|
 Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
 RogerRabbit
Bans for Pleasure
 
Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 1 month
|
 Cordyceps in Washington State 
#7490010 - 10/05/07 09:25 PM (16 years, 6 months ago) |
|
|
Mrs Rabbit found this today while we were looking for Chanterelles in an old growth forest in the Cascade mountains of Washington State. It's the first time I've ever seen such a thing around here. I carefully excavated it and sure enough, it was growing from an insect larvae of some kind. I'll be cloning it in the morning.
RR
   
--------------------
Download Let's Grow Mushrooms
semper in excretia sumus solim profundum variat
"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
Thomas Edison
|
Cryogenicz
what?
Registered: 07/01/04
Posts: 2,421
Loc: Oregon
Last seen: 4 years, 10 months
|
Re: Cordyceps in Washington State? [Re: RogerRabbit]
#7490173 - 10/05/07 11:53 PM (16 years, 6 months ago) |
|
|
Mrs's always find the good shit. (grumble)
-Graham
--------------------
www.MycoPath.com
Mushroom Spawn, Cultures, Fungi Bags, Casings, Master Grain Jars, Bags for In-vitro, Laboratory supplies, and much more!
Mushroom Supplies. Fast Turnaround Times. Great Service.
orders@mycopath.com
enter code shroomery for 10% off product.
www.FungiForum.com
|
 CureCat
Strangest
Registered: 04/19/06
Posts: 14,058
Loc: clawing your furniture
|
Re: Cordyceps in Washington State? [Re: RogerRabbit]
#7490217 - 10/06/07 12:21 AM (16 years, 6 months ago) |
|
|
Hmmm... I bet there is a good key to identifying Cordyceps some where on the web... I'll have to find it later.
Cloning sounds like a fantastic idea. You should probably preserve whatever remains of the fruiting body, since the clone will doubtfully fruit in absence of the insect host.
Good shit. 
--------------------
|
Speeker
Registered: 02/11/04
Posts: 882
Loc: 
|
Re: Cordyceps in Washington State? [Re: CureCat]
#7490262 - 10/06/07 01:00 AM (16 years, 6 months ago) |
|
|
Good find... lucky you.. 
C. militaris is very much like that..
|
Dr. uarewotueat
Peyote Farmer
Registered: 09/02/06
Posts: 16,545
Loc: Uk / Philippines
Last seen: 10 years, 8 months
|
Re: Cordyceps in Washington State? [Re: Speeker]
#7490443 - 10/06/07 03:35 AM (16 years, 6 months ago) |
|
|
amazing 
--------------------
View My Gallery
|
Culland
Stranger
Registered: 09/12/07
Posts: 324
Last seen: 3 months, 2 days
|
|
Thats awesome!
Cul
|
YidakiMan
Stranger
Registered: 09/28/02
Posts: 2,023
|
Re: Cordyceps in Washington State? [Re: RogerRabbit]
#7496867 - 10/08/07 10:09 AM (16 years, 6 months ago) |
|
|
Quote:
RogerRabbit said:
Can I nominate for Pic of the Month if its not in the Pictures forum? 
|
@cro
new name
Registered: 12/07/02
Posts: 1,224
Loc: The PNW
|
Re: Cordyceps in Washington State? [Re: YidakiMan]
#7498865 - 10/08/07 06:32 PM (16 years, 6 months ago) |
|
|
Nice find RR. Rare indeed.
|
RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 1 month
|
Re: Cordyceps in Washington State? [Re: @cro]
#7499597 - 10/08/07 09:30 PM (16 years, 6 months ago) |
|
|
Hey bro, nice to see ya.
I've made two transfers away from bacteria so far, and it's looking like I'm going to have a clean culture after a few more.
RR
--------------------
Download Let's Grow Mushrooms
semper in excretia sumus solim profundum variat
"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
Thomas Edison
|
Hotnuts
old hand
Registered: 02/26/05
Posts: 3,436
Loc: Wild Blue Yawnder
Last seen: 6 days, 23 hours
|
Re: Cordyceps in Washington State? [Re: RogerRabbit]
#7501137 - 10/09/07 10:35 AM (16 years, 6 months ago) |
|
|
Neat!
What does the mycelium look like?
|
RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 1 month
|
Re: Cordyceps in Washington State? [Re: Hotnuts]
#7501194 - 10/09/07 10:51 AM (16 years, 6 months ago) |
|
|
Bright white, provided that's cordyceps mycelium and not mold I have. I've been making transfers away from bacteria contamination every 24 to 30 hours. The mycelium is just now getting established. I'm shooting video of the entire cloning and isolation procedure, so will have pictures to show if things work out as expected.
RR
--------------------
Download Let's Grow Mushrooms
semper in excretia sumus solim profundum variat
"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
Thomas Edison
|
2FiNiTe
ConsideratlyKilling Me
Registered: 06/12/06
Posts: 1,635
Loc: New England
Last seen: 7 years, 7 months
|
Re: Cordyceps in Washington State? [Re: RogerRabbit]
#7502164 - 10/09/07 04:53 PM (16 years, 6 months ago) |
|
|
What altitude and temperature zones did you find it in? Just curious, I'm not sure if you remember our conversation a while ago about acquiring Cordycep Sinesis. The little project I was working on(getting samples from pills) I scrapped it and took your advice and my Lions Mane is on the way. Very interesting about the Pf cake method your doing, I'm thinking of giving it a try.
Anyway, thats an amazing find and I hope your cloning goes well.
--------------------
"Ours is a world of nuclear giants and ethical infants. We know more about war that we know about peace, more about killing that we know about living."
General Omar N. Bradley
|
RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 1 month
|
Re: Cordyceps in Washington State [Re: 2FiNiTe]
#7502206 - 10/09/07 05:04 PM (16 years, 6 months ago) |
|
|
It was found about 35 to 40 miles east of Seattle, at an elevation of 2500 feet above sea level. We're going back to the same area tomorrow to look for more. I don't hold out much hope of finding any since this is our regular mushroom hunting area that we've used for at least 20 years. We'll be getting up at 4 AM so we can get there at the start of daylight. Perhaps now that we've seen one up close, we can have our eyes tuned in better.
RR
--------------------
Download Let's Grow Mushrooms
semper in excretia sumus solim profundum variat
"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
Thomas Edison
|
2FiNiTe
ConsideratlyKilling Me
Registered: 06/12/06
Posts: 1,635
Loc: New England
Last seen: 7 years, 7 months
|
Re: Cordyceps in Washington State [Re: RogerRabbit]
#7502768 - 10/09/07 07:29 PM (16 years, 6 months ago) |
|
|
Thats a pretty low altitude for Cordyceps, very interesting, but then again thats the wrong side of the world too .Again awesome find and I wish you luck.
--------------------
"Ours is a world of nuclear giants and ethical infants. We know more about war that we know about peace, more about killing that we know about living."
General Omar N. Bradley
|
casgoodie
weedwright
Registered: 10/31/06
Posts: 770
Loc: terra
Last seen: 10 years, 5 months
|
Re: Cordyceps in Washington State [Re: RogerRabbit]
#7507590 - 10/11/07 12:28 AM (16 years, 6 months ago) |
|
|
very interesting, thanks for sharing
--------------------
TRAPPED IN LINGUISTIC CONCEPTS
|
FreeSporePrints
Registered: 03/06/05
Posts: 1,139
Loc: Rome, Italy 
Last seen: 11 days, 42 seconds
|
Re: Cordyceps in Washington State [Re: RogerRabbit]
#7507898 - 10/11/07 05:03 AM (16 years, 6 months ago) |
|
|
Great find Roger,
always glad to see your pics, and save the culture 
Fabio
ps. i'll go next week to charge my cc to buy the DVDs, sorry for the delay but i'm totally busy.
|
Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,674
Loc: Montana
|
 Re: Cordyceps in Washington State [Re: RogerRabbit]
#7508514 - 10/11/07 11:18 AM (16 years, 6 months ago) |
|
|
very cool!~
You should go up to Rattlesnake and look for Chanterelles, bro...
they are thick up in there.
tc
--------------------
Laterz, Road
Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!
Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.
|
RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 1 month
|
Re: Cordyceps in Washington State [Re: Roadkill]
#7509040 - 10/11/07 01:58 PM (16 years, 6 months ago) |
|
|
Yea, ya can't hardly walk in that area without stepping on them. I'm burned out already, but of course viv can't ever get enough chanties. I like the Boletes much better, but they're just about done for the season.
RR
--------------------
Download Let's Grow Mushrooms
semper in excretia sumus solim profundum variat
"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
Thomas Edison
|
RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 1 month
|
Re: Cordyceps in Washington State [Re: RogerRabbit]
#7515701 - 10/14/07 12:52 PM (16 years, 6 months ago) |
|
|
Here's a few pictures of the cloning process so far. There seems to be very little information online or in print regarding cordyceps cultivation. I know they do have a 'green mold' stage where the mycelium can produce spores without producing a fruit body first. Hopefully, that's some of what I'm seeing here. The color is blue, but microscopically, they're nothing like penicillium spores. The mycelium itself seems to resemble mold as well, with lots of branching.
The first picture is the next morning after the initial cloning to agar, showing each transfer point surrounded by bacteria. A few transfers later, I've been able to leave most of the bacteria behind, thanks to iodine and peroxide on the cultures, and gentmycin sulphate in the agar.
In the third dish, there are no blue spores in the section labeled X-4, even though the mycelium resembles the other two sections of the dish that are sporulating. I took a few transfers from that section this morning.
The next picture has a section X2A, that has different mycelium than the rest, even though blue spores are showing at the points of transfer. The multiple colonies in the center dishes are not satellite colonies, but were put there by transfers to try to get as many cultures as possible onto the dishes, since I was running out of petris.
The last picture is from transfers that were made 24 hours ago. I took sixty cultures yesterday, three on each of 20 dishes. I transferred once again mycelium from each and every culture I had going. They appear to be starting to sporulate on the oldest part of the culture. I'm still having my doubts whether I have Cordyceps mycelium or mold. I'm on my fifth sleeve of petri dishes for these isolations, but the pictures shown are representative of all.
Hopefully, someone will come along with some knowledge of Cordyceps (John?) and let me know if I'm on the right track here. Otherwise, the pictures are up for the next researcher to study. I'll update as I find out more.
RR
    
--------------------
Download Let's Grow Mushrooms
semper in excretia sumus solim profundum variat
"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
Thomas Edison
|
ohmatic
searcher
Registered: 02/28/04
Posts: 6,742
Loc: europe
|
Re: Cordyceps in Washington State [Re: RogerRabbit]
#7515798 - 10/14/07 01:20 PM (16 years, 6 months ago) |
|
|
THIS is how i like it
--------------------
 MONOTUB tek  HEATBOMB tek
RIP #cultivation! ....can't associate? well FUCK U !
|
|
Threaded
Previous
Index
Next
Edit 
Reply 
whole9
gordonoalberto23
1 2 3 4 all )
Suntzu
doc34