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Offlinewutang
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agar questions for the experienced
    #7498600 - 10/08/07 05:35 PM (16 years, 3 months ago)

i made some agar plates, i scraped spores on it, do they need to be wet to germinate?

also will bruised mushroom tissue still grow on the agar?


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InvisibleMankey
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Re: agar questions for the experienced [Re: wutang]
    #7498611 - 10/08/07 05:38 PM (16 years, 3 months ago)

Take a drop of water to your print and swirl it around with your inoculation loop. That water should be enough to get them started. Just as long as your prepared your agar right they should do fine. When cutting a wedge out of a mushroom bruising is almost unavoidable so yes, bruised tissue will do fine.


Edited by Mankey (10/08/07 05:39 PM)


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OfflineRogerRabbitM
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Re: agar questions for the experienced [Re: Mankey]
    #7498628 - 10/08/07 05:42 PM (16 years, 3 months ago)

The moisture in the agar will germinate the spores. Spores should be transferred with an inoculating loop. Don't scrape them into the agar.
RR


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Invisibleorchidfanatic
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Re: agar questions for the experienced [Re: RogerRabbit]
    #7498758 - 10/08/07 06:13 PM (16 years, 3 months ago)

I got some prints and zig zaged them onto agar with a loop,they looked greasy for the first 3-4 days now they are turning white.. is that contams or do prints on agar do that sometimes.. the "greasy" growths are right along the zig zag and no where else.. maybe I can post a pic..


Edited by orchidfanatic (10/08/07 06:22 PM)


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Invisibleorchidfanatic
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Re: agar questions for the experienced [Re: orchidfanatic]
    #7498809 - 10/08/07 06:23 PM (16 years, 3 months ago)

I think my print was contaminated and just now the spores are germinating .. let me get a pic


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Offlinewutang
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Re: agar questions for the experienced *DELETED* [Re: orchidfanatic]
    #7498830 - 10/08/07 06:25 PM (16 years, 3 months ago)

Post deleted by wutang

Reason for deletion: sorry



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Invisibleorchidfanatic
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Re: agar questions for the experienced [Re: wutang]
    #7498838 - 10/08/07 06:27 PM (16 years, 3 months ago)

I thought it was relevant .. sorry wutang


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Offlinewutang
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Re: agar questions for the experienced [Re: orchidfanatic]
    #7498850 - 10/08/07 06:28 PM (16 years, 3 months ago)

it is related
sorry go on


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Invisibleorchidfanatic
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Re: agar questions for the experienced [Re: wutang]
    #7498916 - 10/08/07 06:44 PM (16 years, 3 months ago)

thanks wutang .. let me know if I am out of line anytime .. you the man
so here is the pic. I did the same procedure with oyster spores and they didn't do this greasy thing .. but now mycelium is there.. just would like an opinion ..

sorry for the poor quality I tried to get it clear.. but you can see the greasyness around the newly formed mycelium..
oh and I never had a problem with spores germinating on plain agar . like RR says, it just takes time ..


Edited by orchidfanatic (10/08/07 06:46 PM)


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Offlinexhooliganx
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Re: agar questions for the experienced [Re: orchidfanatic]
    #7498976 - 10/08/07 07:00 PM (16 years, 3 months ago)

you don't need to add any moisture to agar plates


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