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Invisibletony8404
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So close... i am .. so close..
    #7495835 - 10/07/07 09:57 PM (16 years, 3 months ago)

Well, i have let things settle down a bit i tried to keep busy while these jars are colonizing. I checked them saturday and i found a dud. it had a green spot. took it out asap and kept it in another room to see what happens.

Last night i decided to give them a shake, this morning they were coming back cause if i turn the jar upside down the grains didnt budge. kept its shape.. lol.. let them be all day checked my contaminated jar and the white if crazy and the green like not there.. i just kept it to learn off of but could it be possible for the myselium to kill or take over the green? i mean i noticed totally the white out grew from the shake late last night till late this afternoon. the green did not expand or get deeper green at all. now i cannot say for the center due to not wanting to mess with them that much. they seemed to be holding together tight saying no stop rightnow...


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OfflineChi Ro
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Re: So close... i am .. so close.. [Re: tony8404]
    #7495950 - 10/08/07 12:10 AM (16 years, 3 months ago)

There's this thing that some of us to use around here called the English Language. If you use it, you might get more replies! :eek:


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Invisiblemonstermitch
Growing in Bags Doesn't Work


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Re: So close... i am .. so close.. [Re: Chi Ro]
    #7495977 - 10/08/07 12:30 AM (16 years, 3 months ago)

Well buddy, your English isn't very proper either...

To respond to the first post:
Chances are the white that is growing is not mushroom mycelium,
but rather mold mycelium.

Mold isn't always green, it starts out with white mycelium, which
to an untrained eye can appear to be mushroom mycelium.

Throw that jar away, it's not worth the risk no matter what.


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Offlinetoadstooly
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Re: So close... i am .. so close.. [Re: monstermitch]
    #7496426 - 10/08/07 06:11 AM (16 years, 3 months ago)

i say dig a hole and bury it outside.


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Invisibleorchidfanatic
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Re: So close... i am .. so close.. [Re: Chi Ro]
    #7496448 - 10/08/07 06:23 AM (16 years, 3 months ago)

Quote:

Chi Ro said:
There's this thing that some of us to use around here called the English Language. If you use it, you might get more replies! :eek:




LOL  "that some of us to use"  ??    :blazed:


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Offlineveda_sticks
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Re: So close... i am .. so close.. [Re: toadstooly]
    #7496449 - 10/08/07 06:23 AM (16 years, 3 months ago)

the green you see is the mould producing spores, before mould produces spores it can resemble shroom mycelium. While it is possible that myc can fight contams, at this early stage it is highly unlikely and even if it was, its better to just start from fresh rather than risk contaminating future grows. Suppose that the mould is growing where u cannot see it and u open that jar - then u could end up with spores in your growing area.

It sounds like its just 1 out of (how ever many jars u have) got a contam so atleast u have not lost all.


Also (i may not be absolutly right about this but) tyvek filters or micropore tape cant guarentee to stop the spores.
I dont see what u can learn from letting a contaminated jar grow, unless u have an isolated room that u can look at it under a microscope then disinfect eveyrthing and get rid of the stuff.


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Invisibleorchidfanatic
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Re: So close... i am .. so close.. [Re: veda_sticks]
    #7496454 - 10/08/07 06:24 AM (16 years, 3 months ago)

bury it !


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Invisiblemycocurious
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Re: So close... i am .. so close.. [Re: orchidfanatic]
    #7496622 - 10/08/07 08:20 AM (16 years, 3 months ago)

1. Semi useful tip...
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Keep a couple colored sharpies around your jars, when you see something suspect, draw a circle that is only a little bit bigger than what you're trying to track. Also, keeping a label or piece of masking tape on the jar makes for a great place to put notes, such as when the date you noticed the potential contaminate. It'll help you track if it's growing and how fast which helps eliminate any uncertainty.

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The reason I mentioned that is because a little spec of green could be a tiny sporulation of some trichoderma in your jar or it could have just be a little spec of green shit that was mixed in there with the rye berries. Either way, when you notice a contaminate, take it out of the group of non-contaminated jars and set it aside so it's not included in the "shake rotation" of the rest of them.

If it's a contaminate you can watch it grow out slowly until you decide what to do with it - since as long as the tyvek and any micropore type you're using stay in place, it can't cross contaminate so it's fine to just let it sit and watch it. It's a good experience to see how fast it spreads, what it looks like during it's process, etc.

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You have several options of disposing of it, but each has it's own risk. While "throwing out the whole jar" is a great way to ensure that you don't release the contaminate back into your environment by opening the jar it might not be something you want your garbage man happening upon - both for your security and his health, in some cases.

If you're going to dump the jar in your outdoors, keep in mind that everything you're wearing and your entire body will be "contaminate city" the minute you crack that seal on the jar and you should be prepared to launder those clothes and shower immediately afterwards. Positioning yourself upwind, if at all possible also helps, but I'm the paranoid type.

Either route you go, you'll need to sterilize that jar thoroughly by pressure cooking it or boiling it for 15 minutes or so, fully submerged. (remember, put it in when the water's cold, boil it, and don't take it out until the water is cold again - glass shatters from temperature shocks)

Even if your going to throw the whole jar out, it's better to boil it anyways, grains still in the jar because it'll kill off any surviving mycelium (good or bad) because the last thing you'll want is for it to still be growing in the jar in your trash - lol.


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:justdontknow: Don't mistake my tone for a "matter-of-fact" attitude.  I'm just presenting what I believe to be correct, until I'm corrected...

- How Myco-Curious Prepares Coir & Compost Substrates
- How Myco-Curious Builds A Bulk Humidifier
- How Myco-Curious Builds An Automated Greenhouse
------------------------------------
figgusfiddus said:
Keep in mind that inoculating or whatever in front of a flow hood won't help your bad substrate, your bad inoculant, your bad sterile procedure, etc. etc. etc. It's not a +3 flowhood of magic, it's just a tool.


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Invisiblemycocurious
Mike O. Kuerias
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Re: So close... i am .. so close.. [Re: mycocurious]
    #7496688 - 10/08/07 08:58 AM (16 years, 3 months ago)

The other point I was going to make is always assume you're going to have up to 25% of your jars contaminate.  While I rarely have anywhere near that many - and often enough, no contaminates at all - it is still smart to prepare a surplus just in case so you can still maintain the 4::1 ratio of substrate to spawn inoculate (or 20-25% if you think in percentages) when it comes time to spawn.

For example, I know that each one of my tubs needs 1.5 quarts of rye grain spawn for every 6.0 quarts of compost that I use.  Since I'll be doing six tubs at a time, that means I'll need 9 quarts of rye grain when I'm finished.  However, since every jar technically only about 75% full to allow for shaking and additional growth, that means I'll really need 12 jars fully colonized (12 x 0.75 = 9.0).  Since I want to make sure I allow for some to be lost to "failure to thrive" (contamination, stalling, etc.) I'll inoculate between 14-16 jars, just in case.  If there are no contaminates, I can just make my ratio a little rich on the spawn-side but I'm saving myself the hassle and risk of making my ratio too lean instead.

It's the same in all trades really, brick masons always order between 5-15% more bricks then they calculated they would need just because they know that some of them will break during the building process.

So now you have an excuse to use all that units of measurement math that you learned in high school.  :yesnod:  Remember, always do your measurements in volume (cubic inches, quarts, cups, gallons, etc.) and not in weights because they are non-equivalent and won't give you accurate estimations...


--------------------
:justdontknow: Don't mistake my tone for a "matter-of-fact" attitude.  I'm just presenting what I believe to be correct, until I'm corrected...

- How Myco-Curious Prepares Coir & Compost Substrates
- How Myco-Curious Builds A Bulk Humidifier
- How Myco-Curious Builds An Automated Greenhouse
------------------------------------
figgusfiddus said:
Keep in mind that inoculating or whatever in front of a flow hood won't help your bad substrate, your bad inoculant, your bad sterile procedure, etc. etc. etc. It's not a +3 flowhood of magic, it's just a tool.


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