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Offlinepaligap75
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Registered: 10/04/07
Posts: 4
Last seen: 16 years, 3 months
no growth w/agar
    #7493918 - 10/07/07 10:29 AM (16 years, 3 months ago)

Hi, I've been reading The Shroomery for almost two years now on and off. Using the info from Shroomery posts and other links I've been pretty lucky cultivating mushrooms. The one problem I have is cultivating anything on agar. I mean I can't even get them to contaminate. Does anybody have an idea what I'm doing wrong? I follow the basic agar teks.


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OfflineAndrew47
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Registered: 04/06/06
Posts: 432
Last seen: 14 years, 3 months
Re: no growth w/agar [Re: paligap75]
    #7494025 - 10/07/07 11:08 AM (16 years, 3 months ago)

You should have no problem contaminating agar with a little effort :laugh:

The only way I could see not being able to get contams is if you are cooking your agar without water??


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Offlinewaixingren
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Registered: 03/14/05
Posts: 2,644
Loc: SW Florida
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Re: no growth w/agar [Re: paligap75]
    #7494126 - 10/07/07 11:49 AM (16 years, 3 months ago)

what is your recipe?


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Invisiblegreys
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Registered: 07/16/06
Posts: 44,923
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Re: no growth w/agar [Re: Andrew47]
    #7494129 - 10/07/07 11:49 AM (16 years, 3 months ago)

you ARE adding some malt extract or dextrose or SOME kind of nutrient to the agar before you cook it?


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OfflineHotnuts
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Registered: 02/26/05
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Re: no growth w/agar [Re: greys]
    #7494419 - 10/07/07 01:23 PM (16 years, 3 months ago)

Even a plain water agar will grow fungus and bacteria.


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OfflineHotnuts
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Re: no growth w/agar [Re: Hotnuts]
    #7495238 - 10/07/07 04:52 PM (16 years, 3 months ago)

If you're using water that's been treated from salt for your agar mixture, that could be causing this. That's if you're using a correct agar mixture. There's something in that salt they use for hard water treatment that really has a negative effect on mycelia growth. I had the same problem with a couple of jars of agar and then went to distilled water and haven't had the problem anymore. Absolutely nothing would grow. Not even a cubensis isolate. It's still colonizing the agar and it's been a few weeks. None of the spores that I put on it would grow out at all. Just barely if any. Even spores that are easy to work with.


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Offlinepaligap75
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Registered: 10/04/07
Posts: 4
Last seen: 16 years, 3 months
Re: no growth w/agar [Re: Hotnuts]
    #7495442 - 10/07/07 05:43 PM (16 years, 3 months ago)

Thanks for the quick reply.I live in the Northeast U.S. and the soil is very sandy. So sandy that every 6-7 years it corrodes the hot water tank. So I did a ph test and the water went off the scale past 8.0 which was as high as I could read. So I started using rainwater that I collect, filter and sterilize. I had one success. It was just a drop of spore solution into the large jar of MEA that was left over after I inoculated the smaller ones. The smalls were all failures. But I've never been able to duplicate what I did. I could tell you more about temps and stuff like that but I don't know. I do appreciate your writing back. Thanks again.


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OfflineHotnuts
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Re: no growth w/agar [Re: paligap75]
    #7495903 - 10/07/07 10:50 PM (16 years, 3 months ago)

Actually, that cubensis isolate is dated 8-28 and it still isn't even close to colonizing half of the plate. :rolleyes:


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