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OfflineCulland
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Re: Cordyceps in Washington State [Re: ohmatic]
    #7516064 - 10/14/07 02:42 PM (16 years, 3 months ago)

Thanks for posting those RR, it's very interesting!

Cul


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OfflineRogerRabbitM
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Re: Cordyceps in Washington State [Re: Culland]
    #7522710 - 10/16/07 11:25 AM (16 years, 3 months ago)

The section labeled X2A turned out to be trichoderma and has been discarded. The sections X3 and X4 seem to be contaminant-free cordyceps mycelium, one in the conidial stage, and the other not. I'll be sending these within the next few days for DNA testing.
RR


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Offlinebodynotdead
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Re: Cordyceps in Washington State [Re: RogerRabbit]
    #7569246 - 10/28/07 09:39 AM (16 years, 3 months ago)

Got any news on the DNA testing?

How are you going to fruit?
I never seen growing perimeters for Cordyceps.

And I pay good money for a Culture Syringe $$$


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OfflineRogerRabbitM
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Re: Cordyceps in Washington State [Re: bodynotdead]
    #7569274 - 10/28/07 09:46 AM (16 years, 3 months ago)

Still waiting on the DNA results. They've had the culture for 2 weeks now, so it shouldn't be much longer.

The Cordyceps mycelium has the same medicinal benefits as the fruits, so it isn't necessary to fruit them. Most grow on rye grains until the grains have been consumed(over colonized) by the mycelium. You're then left with a jar of nearly pure cordyceps mycelium that can be dried and ground to powder.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Invisiblepoboy
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Re: Cordyceps in Washington State [Re: RogerRabbit]
    #7569861 - 10/28/07 02:42 PM (16 years, 3 months ago)

Quote:

RogerRabbit said:
You're then left with a jar of nearly pure cordyceps mycelium that can be dried and ground to powder.
RR


I have read different.(In many cases, the solid-substrate grown mycelium we tested was greater than 80% residual grain. However, a bonus to this method is that the extra-cellular compounds are harvested along with the substrate and mycelium.) i found this here http://www.pharmaceuticalmushrooms.nwbotanicals.org/lexicon/cordyceps/hybridcordyceps.htmThe only way they were left with a good out come was with this substrate.So from our substrate testing it was determined that the ideal medium for solid substrate growth of Cordyceps is as follows: 1 part white proso millet (husk on) to 4 parts of white milo (husk on), with the addition of 0.8% w/w of ground oyster shell and 1% w/w vegetable oil (peanut oil or soybean oil). Add water to equal 50% total moisture in the sterilized substrate. Precooking the grain mixture for 4-6 hours prior to sterilization tends to trigger a much faster growth response from the Cordyceps. On this medium, Cordyceps can be grown for long periods of time, allowing nearly complete conversion of the substrate to mycelium and the full expression of secondary metabolites from the Cordyceps. The resultant Cordyceps when grown on this substrate is about 3-4% residual grain, or about 96-98% pure mycelium.


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Edited by poboy (10/28/07 02:49 PM)


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InvisibleCheezit
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Re: Cordyceps in Washington State [Re: poboy]
    #7570461 - 10/28/07 05:44 PM (16 years, 3 months ago)

Must be about the small kernel size. Makes sense.


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InvisibleBlimeyGrimey
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Re: Cordyceps in Washington State [Re: RogerRabbit]
    #7611499 - 11/08/07 02:46 PM (16 years, 2 months ago)

That looks like a Cordyceps militaris due to its orangish color. If it was more yellow i'd say it could be Cordyceps washingtonensis.

here's a link to a neat little PNW Club fungus field key.
PNW Club Fungi Field Key
(Seems I beat you to it CureCat)

Here's another site with a description of militaris:
Cordyceps militaris

I like this excerpt:
"Cordyceps militaris is pretty much the coolest mushroom ever, and I will detail its features in a moment--but first I am obliged to discuss CMS, a common problem among mushroom hunters and mycologists. The acronym stands for Cordyceps Moron Syndrome, and symptoms present in two ways. The CMS sufferer 1) plucks any club fungus instantly from its substrate, regardless of what treasures might be found beneath the mushroom, and 2) cannot manage to take a decent photo of a Cordyceps even when the first symptom is bypassed."

Seems RR doesn't have CMS!


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Edited by BlimeyGrimey (11/08/07 03:03 PM)


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OfflineRogerRabbitM
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Re: Cordyceps in Washington State [Re: BlimeyGrimey]
    #7611642 - 11/08/07 03:16 PM (16 years, 2 months ago)

The DNA results were expected yesterday. I've been checking email every hour to see if they're there yet.

I'm also suspecting C militaris, but there are some subtle differences, particularly in the club part. The DNA should tell for sure.

They're also doing an unrelated metals test on a shiitake mushroom I sent them. I had a telephone book substrate colonizing in an aluminum turkey pan, and after colonization, it had holes eaten all the way through by the mycelium metabolites. The picture is below. Many cubensis growers have seen the same phenomenon. They're testing the fruitbody for the presence of aluminium to see if it gets absorbed by the mycelium and transferred to the fruit. That test is due in ten days.
RR


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semper in excretia sumus solim profundum variat

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OfflineCulland
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Re: Cordyceps in Washington State [Re: RogerRabbit]
    #7612225 - 11/08/07 06:03 PM (16 years, 2 months ago)

Out of curiosity how much does it cost for you to get a DNA test done and test for metals? What kind of lab do you send it to?

Cul


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Invisiblegreys
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Re: Cordyceps in Washington State [Re: RogerRabbit]
    #7612273 - 11/08/07 06:19 PM (16 years, 2 months ago)

Quote:

RogerRabbit said:
The DNA results were expected yesterday. I've been checking email every hour to see if they're there yet.





WoW! good luck! the suspense must be KILLING you!


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Offlinefaceofbear
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Re: Cordyceps in Washington State? [Re: Cryogenicz]
    #7614941 - 11/09/07 12:04 PM (16 years, 2 months ago)

Quote:

Cryogenicz said:
Mrs's always find the good shit. (grumble)

-Graham




ain't it the truth?!

awesome find, excellent pictures.
looks like that took some careful excavation...


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OfflineRogerRabbitM
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Re: Cordyceps in Washington State? [Re: faceofbear]
    #7618356 - 11/10/07 11:30 AM (16 years, 2 months ago)

The DNA test results came back, and unfortunately, I had isolated a non-sporulating form of Penicillium spinulosum rather than the Cordyceps I was after. I've kept all petri dishes since the beginning in the refrigerator, so I'll go back to stage one and try to get the correct culture this time around. The lab, for those of you interested in DNA testing of fungi cultures is Accugenix, located in Newark, DE. The tests aren't cheap.

It's my understanding that cordyceps mycelium can withstand freezing, so I'll try first putting the cultures into a hard freeze, then after thawing, I'll try to isolate all growths that emerge. I also still have what's left of the original Cordyceps, including the insect host, that has been dried and preserved. I'll try some dry culture restoration techniques as well. I really don't want to lose this, as it's a once in a lifetime find, especially for this area.

The metals test on the Shittake is due by the end of next week.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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OfflineCulland
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Re: Cordyceps in Washington State? [Re: RogerRabbit]
    #7618852 - 11/10/07 01:35 PM (16 years, 2 months ago)

Sorry to hear that RR, hope you get it in the next attempt!

Cul


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InvisibleBlimeyGrimey
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Re: Cordyceps in Washington State? [Re: RogerRabbit]
    #7669572 - 11/23/07 09:31 AM (16 years, 2 months ago)

Did the metals test ever come back? I'd like to know how that went.


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Looking for wild Panaeolus cinctulus and Panaeolus olivaceus prints.


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OfflineRogerRabbitM
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Re: Cordyceps in Washington State? [Re: BlimeyGrimey]
    #7670016 - 11/23/07 11:21 AM (16 years, 2 months ago)

I'm still waiting on the metals test. It's overdue, so should be any day. I'll post results as soon as I have them.

I also made up a batch of cricket agar to isolate the cordyceps farther. Some promising growth is occurring, and absolutely no bacteria seems to be growing on the cricket agar. I won't know anything until we've sent off each isolate for DNA testing. This might be something that could actually help isolate other species of mycelium away from contaminants. I used 18 small crickets(live until I pushed the button on the blender) and 20 grams of agar-agar in 600 ml water.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Offlineabesh
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Re: Cordyceps in Washington State? [Re: RogerRabbit]
    #7670056 - 11/23/07 11:33 AM (16 years, 2 months ago)

:puke:

Why crickets?


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OfflineRogerRabbitM
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Re: Cordyceps in Washington State? [Re: abesh]
    #7670181 - 11/23/07 12:07 PM (16 years, 2 months ago)

Because they have them at the pet store. It's too late in the season to collect them outdoors.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Invisiblelegallyhomeless
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Re: Cordyceps in Washington State? [Re: RogerRabbit]
    #7675994 - 11/24/07 08:34 PM (16 years, 2 months ago)

Awesome. I wonder if they can be found in PA


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OfflineRogerRabbitM
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Re: Cordyceps in Washington State? [Re: legallyhomeless]
    #7689794 - 11/28/07 10:31 AM (16 years, 2 months ago)

The results of the metals testing came back. It appears that even though the mycelium metabolites disolved the aluminum, it wasn't conducted into the fruit bodies. Here's the tray the project colonized in, and beside it, the metals testing report. I'll post this over in cultivation also, for those who might not see it here.
RR



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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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InvisibleRoadkillM
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Re: Cordyceps in Washington State? [Re: RogerRabbit]
    #7689844 - 11/28/07 10:43 AM (16 years, 2 months ago)

Quote:

RogerRabbit said:

The results of the metals testing came back. 

It appears that even though the mycelium metabolites disolved the aluminum, it wasn't conducted into the fruit bodies. 





That's great news!~

:smile:


Thank you for taking the time and money to have this tested...

I was always concerned with using disposable alum trays for growing back when I used them....

so I stopped using them many years ago because of my concern...

and also because of the acid like reaction making holes in the pan itself, so I switched to using heavy duty plastics instead.


thanks again!~


tc


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