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Offlineveda_sticks
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green/blue mould - due to bad inoculation
    #7487417 - 10/05/07 05:40 AM (16 years, 3 months ago)

On my last batch, i made up 3 pf tek jars, that i sterilised in my nice new 15psi pressure cooker for an hour.

Well this is what happens when ur inoculations are not sterile enough. I forgot to take a shower, put on clean clothes, forgot to alcohol swap the top of my jars before and after inoc and did not alcohol swab the tinfoil cover.

I believe this is what caused my contamination as my first batch's that i made that was my procedure, even though it was in open air (i complelty bleach cleaned my kitchen on both batchs).

this developed in 1 out of the 3 jars.









Don't skim on your sterile procedures!!!

I am now making myself a nice glovebox out of a 30 litre plastic container. which i will carry out any future inoculations, cloning, printing etc.


The first sign of contam was a very prominant blue spot about 5mm in diameter after about 2 days. Within the next few days the green spores developed along with white mould. It has now been about 7 days and something that i did not expect is happening. It appears that mycelium is growing along with the green mould. after 7 days i would have expected the jar to be colinised with green as a previous contamination did.

I cannot be certain that it is infact mycelium growing. I was about to load it into my pressure cooker, but then thought. Im gonna leave it in an isolated cover and see what happens. in another week i will either pressure cook it, or i may take it outside somewhere and plant it in the ground.


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OfflineRogerRabbitM
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Re: green/blue mould - due to bad inoculation [Re: veda_sticks]
    #7490739 - 10/06/07 08:27 AM (16 years, 3 months ago)

It looks like you have both penicillium(blue) and trichoderma(green) molds. Kitchens and bathrooms are the two worst places to do sterile work. Nice move on making a glovebox.
RR


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