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Invisibleohmatic
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condensation on INCUBATED petri lids
    #7479570 - 10/03/07 02:18 AM (16 years, 5 months ago)

i usually do not incubate petri dishes however i thought about giving it a try this time.

now, condensation was never an issue for me till now, the mycelium likes the incubation temperaturs
but wow the lids are literally misted ! and ofcoz i do not like this !

wtf:

any idea what to do about it ? think i wont incubate them so soon again ...


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OfflineSeussA
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Re: condensation on INCUBATED petri lids [Re: ohmatic]
    #7479856 - 10/03/07 06:38 AM (16 years, 5 months ago)

> any idea what to do about it ?

Turn them upside down.


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OfflineRogerRabbitM
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Re: condensation on INCUBATED petri lids [Re: Seuss]
    #7479883 - 10/03/07 07:13 AM (16 years, 5 months ago)

Turning them upside down tends to force the water to drain from the agar by gravity, drying it out and ruining the mix. Colonize petri dishes in a vertical stack at room temperature. It's the temperature differential between inside and outside the dish that leads to condensation. Having them stacked vertically goes a long way to reducing the temperature differential on the lids.
RR


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Invisibleohmatic
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Re: condensation on INCUBATED petri lids [Re: RogerRabbit]
    #7479912 - 10/03/07 07:31 AM (16 years, 5 months ago)

definitely wont incubate any petri dish soon anymore, also i am not for stacking any dishes upside down
for reason rogermentioned.


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OfflineAndrew47
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Re: condensation on INCUBATED petri lids [Re: ohmatic]
    #7479924 - 10/03/07 07:34 AM (16 years, 5 months ago)

funny you mention this, I incubated my petris for the first time last night with your "heatbomb" and woke up to find mine like yours :laugh:


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Invisibleohmatic
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Re: condensation on INCUBATED petri lids [Re: Andrew47]
    #7479929 - 10/03/07 07:39 AM (16 years, 5 months ago)

yeah dont do it man, the incubation seems to fuck things up quite some.

roomtemp is the way to go, hope u like 'da bomb :smile:


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OfflineHotnuts
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Re: condensation on INCUBATED petri lids [Re: ohmatic]
    #7479940 - 10/03/07 07:44 AM (16 years, 5 months ago)

I like to keep mine at around 75 degrees. In order to prevent condensation; the plates need to be stored in a container that's used as an insulator. I stack the plates in a clean towel and then load them in the container stacked vertical.

You can stack the plates on top of one another, then wrapped up in a clean towel well to get rid of that condensation. Usually after 1 day it's gone.

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OfflineHotnuts
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Re: condensation on INCUBATED petri lids [Re: Hotnuts]
    #7480071 - 10/03/07 08:39 AM (16 years, 5 months ago)

By the way, leave them stacked right side up until the condensation's gone.

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OfflineSeussA
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Re: condensation on INCUBATED petri lids [Re: RogerRabbit]
    #7480166 - 10/03/07 09:17 AM (16 years, 5 months ago)

> Turning them upside down tends to force the water to drain from the agar by gravity, drying it out and ruining the mix

Really? I've never noticed any problems, but it has been years since I have played...


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Re: condensation on INCUBATED petri lids [Re: Hotnuts]
    #7480412 - 10/03/07 10:49 AM (16 years, 5 months ago)

Quote:

Hotnuts said:
By the way, leave them stacked right side up until the condensation's gone.




thatll just force all the water on the lid to run down on the agar's surface and create quite a pool there ..
dont think thats such a good idea isit ?


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OfflineHotnuts
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Re: condensation on INCUBATED petri lids [Re: ohmatic]
    #7480558 - 10/03/07 11:26 AM (16 years, 5 months ago)

Not if you leave them right side up. Just like they are pictured above. Don't store them vertically until the condensation's gone. If you stack them on top of one another in a towel and then wrap the towel around the stack, the condensation will subside in a day or 2.

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OfflineRogerRabbitM
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Re: condensation on INCUBATED petri lids [Re: ohmatic]
    #7481418 - 10/03/07 03:07 PM (16 years, 5 months ago)

Quote:

ohmatic said:
Quote:

Hotnuts said:
By the way, leave them stacked right side up until the condensation's gone.




thatll just force all the water on the lid to run down on the agar's surface and create quite a pool there ..
dont think thats such a good idea isit ?




That isn't what will happen. The condensation was caused by moisture that was forced out of the agar, so by leaving the dish right side up, the moisture will re-absorb back into it.
RR


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InvisibleArp
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Re: condensation on INCUBATED petri lids [Re: ohmatic]
    #7481712 - 10/03/07 04:37 PM (16 years, 5 months ago)

i use glass dishes and i don't see so much condensation. i keep mine in temperatures around 28-29c. don't know if that would solve your problem, but might be worth giving a shot.

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InvisibleArp
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Re: condensation on INCUBATED petri lids [Re: Arp]
    #7481731 - 10/03/07 04:43 PM (16 years, 5 months ago)

found this:
http://www.springerlink.com/content/dxm8p4625t8l280q/
"Placement of polymer discs on the top of Petri dishes is a relatively simple method that can be used to buffer the lid from small temperature changes and minimize condensation problems."

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