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Invisibletony8404
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Registered: 06/14/05
Posts: 1,323
Are my jars done for?
    #7481566 - 10/03/07 03:54 PM (16 years, 3 months ago)

Well, this past sunday inoculated some rye jars with some LC i had sitting for about a month. the lc sat in the fridge for about 3 weeks. I have my jars sitting in a rubbermaid with a tub under that with a fish tank heater, the temp is 82-86 degrees. i have had the jars sitting there since sunday around 3 is when i finished. today should really be the third day of incubation.. but i have not seen any signs of life as of yet...should i start to worry bout the jars???

i am starting to think the lc culture i had going had died out or cause it was in the fridge being cold may have something to do with the growth starting up again.. i should have let the lc sit out till room temp maybe.. anythoughts??? i was using cambodian strain.


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Offlinepazzy
Always Hardcore


Registered: 08/24/07
Posts: 1,360
Last seen: 13 years, 2 months
Re: Are my jars done for? [Re: tony8404]
    #7481620 - 10/03/07 04:13 PM (16 years, 3 months ago)

I have never refrigerated an LC ever. Give the jars a whole week then start to worry.


--------------------
Are you happy for a miracle?

:yinyang2:

Absolutely no source checks/discussions.


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Offlinefallingaway24
catatonic selfmedicator

Registered: 12/25/04
Posts: 419
Last seen: 9 years, 6 days
Re: Are my jars done for? [Re: pazzy]
    #7481634 - 10/03/07 04:17 PM (16 years, 3 months ago)

3 days isn't alot of time, sometimes you can see myc in 2-3 days but its not uncommon to take a week or so....stay busy and check them in a couple more days....


--------------------
"Most people don't know how they're gonna feel from one moment to the next. But a dope fiend has a pretty good idea. All you gotta do is look at the labels on the bottles." ~drugstore cowboy~


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InvisibleStriplessRED

Registered: 09/05/07
Posts: 562
Loc: Helena
Re: Are my jars done for? [Re: fallingaway24]
    #7481665 - 10/03/07 04:25 PM (16 years, 3 months ago)

You don't need to put the LC's in a fridge, in fact it would be better if you didn't.
Also after 10 days if you see no signs of life, you should start to worry. Your jars are probably fine though.


Good luck!


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OfflinehardwoodAl
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Registered: 08/23/07
Posts: 65
Last seen: 16 years, 3 months
Re: Are my jars done for? [Re: StriplessRED]
    #7481700 - 10/03/07 04:33 PM (16 years, 3 months ago)

I think he meant a fridge as a grow area, unplugged, not cold. his temps in the 80's.
You need to be patient. three days is way to anxious to post "if theyer alright." it can take two weeks to see obvious myc growth.invisable fuzz might start first depending strain.


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Offlinebtb103
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Registered: 02/17/07
Posts: 229
Last seen: 10 years, 8 months
Re: Are my jars done for? [Re: hardwoodAl]
    #7481778 - 10/03/07 05:00 PM (16 years, 3 months ago)

82-86 is a little high. You want to keep temps as close to 80 as possible. Lower than 80 is better than higher than 80. The higher you have it, the more welcome contams are to your myco party. Mycelium produces a good amount of heat on its own. Don't be dismayed at three days, some of my jars have taken over a month to fully colonize (without sings for a week). Patience is key. :smile:


--------------------
In bewilderment, I realized I am the endless sea.


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Invisibletony8404
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Posts: 1,323
Re: Are my jars done for? [Re: btb103]
    #7481991 - 10/03/07 06:12 PM (16 years, 3 months ago)

thanks for the input guys.. i just got home and checked and all 14 of my rye jars have started up with mycelium .. not a lot but its there .. wooo hooo..


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Invisiblemycocurious
Mike O. Kuerias
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Registered: 02/09/07
Posts: 1,265
Re: Are my jars done for? [Re: tony8404]
    #7482024 - 10/03/07 06:20 PM (16 years, 3 months ago)

Next time, IMHO, skip the liquid culture and the incubators, both serve a specific purpose but neither of them have anything to do with speeding up colonization.

And while refridgeration is a great way to provide semi-long term storage for liquid cultures, a "household" refridgerator is a very hostile environment with the internal temperatures swinging from 20(F) - 65(F) depending on it's usage.

---
A trip to the grocery store can cause the temps to rise as it works like mad to chill all the newly loaded goodies...

Conversely, placing something in the fridge from the freezer will cause the temperatures of everything nearby to drop rapidly until that frozen bird/etc. equalizes to the same temp as everything else in the unit.

:soapbox:


--------------------
:justdontknow: Don't mistake my tone for a "matter-of-fact" attitude.  I'm just presenting what I believe to be correct, until I'm corrected...

- How Myco-Curious Prepares Coir & Compost Substrates
- How Myco-Curious Builds A Bulk Humidifier
- How Myco-Curious Builds An Automated Greenhouse
------------------------------------
figgusfiddus said:
Keep in mind that inoculating or whatever in front of a flow hood won't help your bad substrate, your bad inoculant, your bad sterile procedure, etc. etc. etc. It's not a +3 flowhood of magic, it's just a tool.


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Offlinefuturewolf42o
Le Jooses!
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Registered: 03/30/06
Posts: 470
Last seen: 15 years, 8 months
Re: Are my jars done for? [Re: mycocurious]
    #7482047 - 10/03/07 06:25 PM (16 years, 3 months ago)

Quote:

mycocurious said:
Next time, IMHO, skip the liquid culture and the incubators, both serve a specific purpose but neither of them have anything to do with speeding up colonization.

And while refridgeration is a great way to provide semi-long term storage for liquid cultures, a "household" refridgerator is a very hostile environment with the internal temperatures swinging from 20(F) - 65(F) depending on it's usage.

---
A trip to the grocery store can cause the temps to rise as it works like mad to chill all the newly loaded goodies...

Conversely, placing something in the fridge from the freezer will cause the temperatures of everything nearby to drop rapidly until that frozen bird/etc. equalizes to the same temp as everything else in the unit.

:soapbox:





i was under the impression that lc inoculation sped up colonizing time by alot.


--------------------
cheese-it!


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Offlinepazzy
Always Hardcore


Registered: 08/24/07
Posts: 1,360
Last seen: 13 years, 2 months
Re: Are my jars done for? [Re: futurewolf42o]
    #7482076 - 10/03/07 06:30 PM (16 years, 3 months ago)

LC does indeed speed up colonization by a large amount. The spores germinate in the LC and can start growing when they are used to inoculate. When you inoculate with spores you have to wait an additional 5-ish days for the spores to germinate.

If you are planning on only doing one grow though an LC is rather pointless. Since the spores will germinate in 5 days, and then get transferred which takes more or about the same amount of time as inoculating with spores.


--------------------
Are you happy for a miracle?

:yinyang2:

Absolutely no source checks/discussions.


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Invisiblemycocurious
Mike O. Kuerias
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Registered: 02/09/07
Posts: 1,265
Re: Are my jars done for? [Re: futurewolf42o]
    #7482107 - 10/03/07 06:40 PM (16 years, 3 months ago)

Quote:

futurewolf42o said:i was under the impression that lc inoculation sped up colonizing time by alot.




Liquid cultures are a great way to grow out a larger liquid inoculation medium to expand supply. Liquid cultures are also great for temporary long-term storage of a potential isolate for future use.

However, using a liquid culture to cut colonization times is a very delicate balance of the moisture of the grains with the large amounts of sugary medium used to grow your liquid culture. And the uncolonized portions of your liquid culture make for a great culture for whatever other contaminates may have survived pressure cooking. It's risky...


--------------------
:justdontknow: Don't mistake my tone for a "matter-of-fact" attitude.  I'm just presenting what I believe to be correct, until I'm corrected...

- How Myco-Curious Prepares Coir & Compost Substrates
- How Myco-Curious Builds A Bulk Humidifier
- How Myco-Curious Builds An Automated Greenhouse
------------------------------------
figgusfiddus said:
Keep in mind that inoculating or whatever in front of a flow hood won't help your bad substrate, your bad inoculant, your bad sterile procedure, etc. etc. etc. It's not a +3 flowhood of magic, it's just a tool.


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