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OfflineMFKDGAF
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Grain-to-Grain
    #7480906 - 10/03/07 12:37 PM (16 years, 3 months ago)

Ok I PCed 9 grain jars and inoculated all of them with about 3cc. Only 2 of them colonized, so I want to see if it was just some dead spores in the other 7 or if they are just bad jars. And instead of using up all my spores I figured I'd try grain-to-grain but my main question with this is
  • How much of the colonized grain should I put in the jar I am trying to colonize? The jar size is quart.
  • If the jars do not colonize can I pick out the colonized grain or is it just a loss?


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InvisibleCaptain Cubensis
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Registered: 09/18/07
Posts: 648
Re: Grain-to-Grain [Re: MFKDGAF]
    #7480921 - 10/03/07 12:40 PM (16 years, 3 months ago)

If there are no contams, then the jars are good, and the spores were bad, or slow, some jars take longer.

Use about a heaping tbsp for G2G transfers.

And yes, you can transfer only the colonized grains, but it is better to wait for 100% colonization before opening the jars.


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OfflineCaptainLinger
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Re: Grain-to-Grain [Re: Captain Cubensis]
    #7482807 - 10/03/07 09:22 PM (16 years, 3 months ago)

No, you won't be able to pick out the colonized grains, and I have absolutely no idea why CC would tell you you could. I'd definitely recommend G2Ging those bad boys, though honestly I'd just make new grain jars. 50 cents worth of material for a lot less effort.

Picking through the jar won't work. If mycelium isn't growing, there's either a very strong contamination, or the mycelium is dead. Former means you'll transfer the contamination with it, latter means...well, it's dead.


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OfflineCryogenicz
what?
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Re: Grain-to-Grain [Re: CaptainLinger]
    #7482982 - 10/03/07 10:05 PM (16 years, 3 months ago)

only G2G healthy fully colonized spawn.

like Captianinger said... make new jars.

-Graham


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InvisibleCaptain Cubensis
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Registered: 09/18/07
Posts: 648
Re: Grain-to-Grain [Re: CaptainLinger]
    #7489290 - 10/05/07 05:13 PM (16 years, 3 months ago)

What? I said do a grain to grain, never anything about picking out individual grains, where did that come from?

He meant if 1/2 the jar colonizes, can I use that half for G2G. Why couldn't you do that?

I said wait for 100% colonization before using it for G2G, but you can use only what is colonized, although it is not advised, but if your tek is sterile, what difference would exposed grains in the first jar make when you are dumping the G2G on exposed grain anyway?


--------------------
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Get 350lbs shipped to your door for only $100!
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www.hydroponics.net/i/200002
The Cyclestat 4P, a great repeat cycle timer made in the USA.


Edited by Captain Cubensis (10/05/07 05:16 PM)


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OfflineFunkatron9000
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Re: Grain-to-Grain [Re: Captain Cubensis]
    #7489336 - 10/05/07 05:29 PM (16 years, 3 months ago)

I have tried G2G with jars not 100% done and have had mixed results.
Some do great while others don't.


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OfflineVisionsToReality
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Registered: 09/22/07
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Last seen: 16 years, 1 month
Re: Grain-to-Grain [Re: Funkatron9000]
    #7489376 - 10/05/07 05:52 PM (16 years, 3 months ago)

I found this pretty helpful for grain to grain. There's a section on it here:

http://www.shroomery.org/forums/showflat.php?Cat=0&Board=2&Number=7393204&page=0&fpart=1


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