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DigitalNomad
Neophyte


Registered: 08/03/07
Posts: 158
Last seen: 12 years, 9 months
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Moving up to bulk, last minute questions!
#7477226 - 10/02/07 12:02 PM (16 years, 3 months ago) |
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Okay, so I've decided to try my hand at bulk, and everything is prepared, I just have a few questions.
1. I'm going to be using a sheep manure/coir mix. should I mix it all together and pasteurize it? Or keep them separate and do it? Should I even bother with the coir, even though I've heard it's good for 'fluffing' up the substrate.
2. How sterile does this process have to be? The same methods for inoculation, or since I'm dealing with fully colonized myc, will just standard cleanliness be acceptable.
3. I have just shy of 3 quarts of rye spawn to play with. Using a 19x13 inch tub, what would be the most ideal depth for production, without compromising my chances of successful colonization. I think i did the math to about 4 inches, but can someone with more experience double check it?
Thanks guys!
-------------------- Here's to the first step...
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mycocurious
Mike O. Kuerias



Registered: 02/09/07
Posts: 1,265
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Re: Moving up to bulk, last minute questions!- [Re: DigitalNomad]
#7478033 - 10/02/07 04:30 PM (16 years, 3 months ago) |
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1. I'm a big fan of having a diverse food-source for the growing mycelium, so yea, coir would be a great addition to any substrate (i even add it to my homemade compost)
2. hydrate each, separately, to field capacity and then mix together in whatever ratio you'd like and pasteurize together.
3. depth of spawn....
If you go with the standard 4(substrate):1(spawn) ratio then you're looking at 12 quarts total to work with...
12 quarts = 693 cubic inches 19" x 13" x 1" = 247 cubic inches
--- so if stick with the standard ratio, you're looking at a total depth of only 2.75" for your substrate (9 quarts) + grains (3 quarts)... which is a little on the thin side so if you had a smaller container it might be a little better but I think you'd be fine. Just make sure to only make the casing layer be 3/4-1.0" or less...
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Don't mistake my tone for a "matter-of-fact" attitude. I'm just presenting what I believe to be correct, until I'm corrected... - How Myco-Curious Prepares Coir & Compost Substrates - How Myco-Curious Builds A Bulk Humidifier - How Myco-Curious Builds An Automated Greenhouse ------------------------------------ figgusfiddus said: Keep in mind that inoculating or whatever in front of a flow hood won't help your bad substrate, your bad inoculant, your bad sterile procedure, etc. etc. etc. It's not a +3 flowhood of magic, it's just a tool.
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DigitalNomad
Neophyte


Registered: 08/03/07
Posts: 158
Last seen: 12 years, 9 months
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Re: Moving up to bulk, last minute questions!- [Re: mycocurious]
#7478057 - 10/02/07 04:36 PM (16 years, 3 months ago) |
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Thanks for the advice, looks like I was doing it right then.
As for the depth, maybe if I just sectioned off a smaller portion of the tub? So, instead of 13x19, I made a barrier so it would only be 13x17? and possibly took a little more of a risk and went with a 1:5 mixture of spawn:substrate?
I just don't feel like not using a perfectly good tub I purchased.
-------------------- Here's to the first step...
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dr_asterisk
Simplton



Registered: 06/22/07
Posts: 155
Last seen: 1 year, 1 month
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Re: Moving up to bulk, last minute questions!- [Re: DigitalNomad]
#7478091 - 10/02/07 04:48 PM (16 years, 3 months ago) |
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I'm still very new to bulk, on my 4 generation, So I feel I'm still very sensitive to issues that the more experienced grows have very little trouble with. That being said, let me caution you on using a high spawn to sub ratio. The high my ratio the higher my contamination percentage. At 1:3 I have yet to have any contamination issues, the spawn cuts through the substrate and seems to be much more forgiving with saturation levels and sterility of preparation.
Just my 2 cents
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VisionsToReality
RIBBONS


Registered: 09/22/07
Posts: 1,083
Last seen: 16 years, 1 month
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Re: Moving up to bulk, last minute questions!- [Re: dr_asterisk]
#7478546 - 10/02/07 07:34 PM (16 years, 3 months ago) |
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Don't you mean a low spawn to sub ratio, rather than high? As in, less spawn per sub = more possibility of contamination?
-------------------- Life is one big road with lots of signs, So when you're ridin' through the ruts, Don't you complicate your mind. Flee from hate, mischief and jealousy Don't bury your thoughts, Put your vision to reality, yeah!
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DigitalNomad
Neophyte


Registered: 08/03/07
Posts: 158
Last seen: 12 years, 9 months
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Re: Moving up to bulk, last minute questions!- [Re: VisionsToReality]
#7479023 - 10/02/07 10:06 PM (16 years, 3 months ago) |
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Final question, as I'll just adjust my tub size accordingly.
How do you keep it sterile after the pastuerization? I'm using the pillowcase method and I have to let it drain overnight, but at the same time, isn't that pretty much just undoing everything I just did?
-------------------- Here's to the first step...
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dr_asterisk
Simplton



Registered: 06/22/07
Posts: 155
Last seen: 1 year, 1 month
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Re: Moving up to bulk, last minute questions!- [Re: VisionsToReality]
#7481163 - 10/03/07 01:50 PM (16 years, 3 months ago) |
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Quote:
VisionsToReality said: Don't you mean a low spawn to sub ratio, rather than high? As in, less spawn per sub = more possibility of contamination?
yes, the less spawn to bulk as in 1:5 or 1:10 the higher my contamination percentage, But I would consider that a high ratio, 1:1 ratio being the lowest. Any way that being said, I have done a Pillow-Case similar method, difference being I used paint strainer bags. I had no reason to leave them out over night, 30 mins to drain, squeeze out as much moisture as I possibly could, drain for 10 mins, cool for 2 hours. I have also considered after squeezing out all the moisture I could, placing it in a bag to allow it to cool. Personally I prefer the Reynolds oven bags to the Pillow Case, You just have to get your water content right prior to pasteurization.
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Pr0_X
CultivationLifer



Registered: 01/18/06
Posts: 617
Loc: Igloo's and polar bears
Last seen: 16 years, 1 day
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Re: Moving up to bulk, last minute questions!- [Re: dr_asterisk]
#7481270 - 10/03/07 02:23 PM (16 years, 3 months ago) |
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Coir retains moisture alot better then straw does so the idea of useing it with your bulk mixture is ideal, especially when you want to flush the living hell out of the sub.
A perfect mixture would be 1cup of hpoo to 2 cups of straw aswell as a half a cup of coir to every two cups of straw...
I tryed rewriting that to be atleast confusing as possible but it still sounds awary. Anwyho, that's what I use but for you im not really sure with sheep poo seeing as ive never used it. The sterile technique does not matter much if at all, bring a pot of water to a boil for 5 minutes take it off, submerge your mixture for a hour and a half, take it out, squeeze the living hell out of it until all excess moisture is gone, wait to cool and spawn.
From my experience the tighter you pack your bulk the bigger and more prolific the flushes so i'd recommended you try it out for yourself.
3qt's of rye should be able to be streched to accomplish a full run of your substrate.
-------------------- It's okay to hurt my feelings cause you know, they're so numb anyway. but I guess it's what I get for being to fuckin stupid to stay away - Jake - Support the FSR at www.fsrcanada.com and www.fsre.nl
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dr_asterisk
Simplton



Registered: 06/22/07
Posts: 155
Last seen: 1 year, 1 month
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Re: Moving up to bulk, last minute questions!- [Re: Pr0_X]
#7481510 - 10/03/07 03:36 PM (16 years, 3 months ago) |
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So are you saying 2:4:1 hpoo:straw:coir?
That sounds interesting.
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