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Offlineaspore
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Easy Clone v.1.0
    #7474529 - 10/01/07 03:52 PM (16 years, 4 months ago)

My cheap attempt at cloning:

1. Fill a syringe with spring water and wrap in aluminum foil. PC for 15 mins. Let cool.
2. Remove the cap from choice fruit.
3. Being sterile (as always!) insert the syringe longways down the stem of the fruit, go as far as you can.
4. Inject a little water, then suck it back up. Repeat untill you see mycelum specs floating around in the syringe. (once might be enough)
5. inoculate LC and boom, clone.



My rough pictorial of the process. Assuming the inside of a mushroom is contaminant free, this should be a good way to clone. I'm trying it with pictures once I get some damned batteries.

!aspore


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Invisiblejeetered
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Re: Easy Clone v.1.0 [Re: aspore]
    #7474535 - 10/01/07 03:55 PM (16 years, 4 months ago)

u can get core sample tools at hobbylobby, core sample clones work great like this also, essentially that is what you're doing anyhow :smile:


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OfflineMFGFA37
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Re: Easy Clone v.1.0 [Re: jeetered]
    #7474659 - 10/01/07 04:44 PM (16 years, 4 months ago)

Interesting... seems safer than splitting the stipe and getting a chunk w/exacto or needle. Would like to see those pictures too!


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There is no try, only do. -Yoda-

Take no thought for tomorrow, tomorrow shall take thought for itself. Sufficient for one day are the thoughts/worries for that day. Any thought past this is useless. -Ole Jesus-


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InvisibleMagash
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Re: Easy Clone v.1.0 [Re: aspore]
    #7474704 - 10/01/07 05:03 PM (16 years, 4 months ago)

Shit you know how lazy I was back in the day. I have this in the faq someplace but anyway take a syringe and pc it and the tip must be facing up or the water boils out. Put the sterile syringes in a glove box with some cakes that are colonized and put chunks of myc in the syringes and shake. :wink:

Laugh your ass off but it worked :tongue:  Same thing should work fine with shrooms.


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All creatures tremble when faced with violence. All creatures fear death, all love life. If we can only see ourselves in others, then how could we possibly hurt another creature?


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InvisibleThePyschonaut52
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Re: Easy Clone v.1.0 [Re: Magash]
    #7474973 - 10/01/07 06:26 PM (16 years, 4 months ago)

shit magash, thats what i just did:lol:


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"In god we trust..."


-I guess we're screwed.


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Offlineaspore
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Re: Easy Clone v.1.0 [Re: ThePyschonaut52]
    #7475540 - 10/01/07 09:14 PM (16 years, 4 months ago)

No glovebox needed here. It helps, but like shooting up jars, is not neccessary.


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Offlinexhooliganx
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Re: Easy Clone v.1.0 [Re: aspore]
    #7475631 - 10/01/07 09:38 PM (16 years, 4 months ago)

yeah core biopsy has been done before. I personaly prefer cutting a section from the inside, but I have scalples. just proffed a clone lc probably going to throw it out and use one of the jars for grain lc.

I still don't understand why people don't bother usng a glove box. you can make one for under 10 bucks.


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Invisiblejeetered
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Re: Easy Clone v.1.0 [Re: xhooliganx]
    #7475881 - 10/01/07 11:05 PM (16 years, 4 months ago)

yeah glove boxes are like easiest thing to build ever, but not really needed unless fooling with something like this. Which at some point bcomes very necessary if you're going to commit to a lifetime of cultivating any species.


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Offlineveda_sticks
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Re: Easy Clone v.1.0 [Re: jeetered]
    #7476377 - 10/02/07 04:43 AM (16 years, 4 months ago)

THere is already a tek similar to this one, clean stem with alcohol then stab syringe thru stem, there should now be a peice of tissue in the syringe, then inoc a LC.

I agree that u really should use a glovebox for all sterile procedures unless u have an incredibly clean and draft free room.

I have learned this after a batch of 4 pf teks i made, 3 became contamed, then my last batch of 3 1 became contamed. I thought it was because i was steam sterilising, so bought a pressure cooker and i still managed to get a contamed jar.


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PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


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Offlineaspore
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Re: Easy Clone v.1.0 [Re: veda_sticks]
    #7476440 - 10/02/07 05:44 AM (16 years, 4 months ago)

Yeah, I agree with the glovebox, and I used one with this Tek last night, but to each his own.

Have you guys tried to stab a stem and get a piece of it inside the syringe? That shit is harder than it looks. Also, I believe that TEK was done free of a glovebox, just like mine.

This is a little different than anything I have seen yet, or I wouldn't have even posted this.

I did this last night, I cut the cap off of a freshly picked fruit, then quickly inserted my needle from the cut point down the length into the base area. (Now thinking about it you don't even have to cut the cap, just insert it from the bottom up into the base a bit). Then I injected about .5 cc's, then sucked it back up quick. This held just a few specs of mycelium. I have noticed the inside of fruits are fuzzy, and possibly easier to work loose than trying to cut a piece off or stab a piece or whatever. I then shot this into an LC. I'm not saying this is the best, or even good at all, but It might work for some.

I still advise to build a glovebox for anyone who wishes to be dedicated to this hobby like Jeetered said, but once again, it's not necessary for this Tek. This helps out the new growers in getting a good first LC going, with little more supplies than what they already have from doing PF.

!asp@re


Edited by aspore (10/02/07 05:46 AM)


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Offlineaspore
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Re: Easy Clone v.1.0 [Re: aspore]
    #7476453 - 10/02/07 05:52 AM (16 years, 4 months ago)

Thinking about it you might be able to just stab from the very center point of the cap, down into the hollow of the stem. The stem itself is like a tube (for most varieties), and inside it is sterile. So the cap would act as a sort of inoculation port, which reduces the need for a glovebox even more, you don't even have to pick the fruit to do it through the cap, it's held in place for you then!

!asp0re


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