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Offlinewhiterussia
Stranger


Registered: 07/12/07
Posts: 117
Last seen: 15 years, 8 months
all 4 of my jars contamed...wtf
    #7473678 - 10/01/07 11:57 AM (16 years, 3 months ago)

So I'm getting frustrated and losing faith I can do this. I soaked my WBS for 24 hours then rinsed it simmerd it then let it dry out for a day then loaded it into my quart jars 3/4 and put it in the PC for 1 hour 30 min. I think what I may have done wrong was take the jars out while they where still hot, should I let them cool in the PC? also my strain TC I made into Apple juice LC.


also what does it mean if there growth in the jar of myc and then X days later you see contams does this mean that my LC is ok?(it looks ok as far as I can tell in the bottle) or does this mean that it was the Tek I used.


thank you for your time, very disappointing AHHh!


--------------------
When you make your peace with authority, you become authority. - Jim Morrison

The most loving parents and relatives commit murder with smiles on their faces. They force us to destroy the person we really are: a subtle kind of murder. - Jim Morrison


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Offlinekrypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 3 months
Re: all 4 of my jars contamed...wtf [Re: whiterussia]
    #7473806 - 10/01/07 12:24 PM (16 years, 3 months ago)

I would say as far as the LC goes, it could go either way but I wouldn't be worried, more than likely I would think your LC is fine.

Why did you let them dry out for a day? You're only supposed to do it for 20-30 min. or so from what I have read. Also did you rinse it again after simmering? Another note, did you remove all the sunflower seeds and floaters (from what I've read these may not be bad at all, and in one tech it says the help, but I've always removed them and had no problems. How are you sealing your jars? Everything else that you did seems fine, but we'd need more info on your setup to find possible causes of contaminates.

edit: and what is your procedure for inoculating? I always wipe the jars with an alcohol pad, flame the needle, wipe needle with alcohol to cool it down, then inject, pull out whipe again with alcohol, inject more etc. At various points I'll flame again, every 2-3 jars just in case. Afterwards I apply some Pore tape or w/e it's called I forget. 3M Gentle Paper tape from Nexcare, I get it at walmart in the medical isles.

And wipe all surfaces you'll be working with down with alcohol and spray lysol before and during.


Edited by krypto2000 (10/01/07 12:27 PM)


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OfflineVegan
using the searchbutton
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Registered: 08/16/07
Posts: 352
Last seen: 14 years, 6 months
Re: all 4 of my jars contamed...wtf [Re: whiterussia]
    #7473857 - 10/01/07 12:37 PM (16 years, 3 months ago)

Quote:

whiterussia said:
So I'm getting frustrated and losing faith I can do this. I soaked my WBS for 24 hours then rinsed it simmerd it then let it dry out for a day then loaded it into my quart jars 3/4 and put it in the PC for 1 hour 30 min. I think what I may have done wrong was take the jars out while they where still hot, should I let them cool in the PC? also my strain TC I made into Apple juice LC.


also what does it mean if there growth in the jar of myc and then X days later you see contams does this mean that my LC is ok?(it looks ok as far as I can tell in the bottle) or does this mean that it was the Tek I used.


thank you for your time, very disappointing AHHh!




I too am having trouble with the WBS and contams..

I know that I lost a few cause I did not tighten the lids ..I was so use to leaving them on the loose side from doing brf jars..
if you think you did every thing right(looks like you did)
it has to be in the inoculation..same syringe??
same LC ..?/

Did you get any growth of myc or did they contam immediatly with mold..Ive had some start colonizing and go green on me..

how the poly fill hole?? is it stuffed tight??

just some thoughts..


--------------------
I came, I saw , I came back


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Invisiblemycocurious
Mike O. Kuerias
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Registered: 02/09/07
Posts: 1,265
Re: all 4 of my jars contamed...wtf [Re: Vegan]
    #7473937 - 10/01/07 01:03 PM (16 years, 3 months ago)

as far as i know...

you cannot differentiate contaminate mycelium from your desired strain's mycelium. (nearly) all mycelium is white-ish and the only way to know if a liquid culture is contaminated is...to grow it out.

...that, primarily is the reason I think I've never tried one yet.

But, as always, YMMV.


--------------------
:justdontknow: Don't mistake my tone for a "matter-of-fact" attitude.  I'm just presenting what I believe to be correct, until I'm corrected...

- How Myco-Curious Prepares Coir & Compost Substrates
- How Myco-Curious Builds A Bulk Humidifier
- How Myco-Curious Builds An Automated Greenhouse
------------------------------------
figgusfiddus said:
Keep in mind that inoculating or whatever in front of a flow hood won't help your bad substrate, your bad inoculant, your bad sterile procedure, etc. etc. etc. It's not a +3 flowhood of magic, it's just a tool.


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Invisibledysphoria
lost soul

Folding@home Statistics
Registered: 06/04/03
Posts: 1,651
Re: all 4 of my jars contamed...wtf [Re: mycocurious]
    #7474058 - 10/01/07 01:38 PM (16 years, 3 months ago)

as an ardent LC grower for many years, i recently switched back to my roots of doing multispore, for the reasons mentioned above. it all seems like the sky is the limit and thousands of grain jars could be colonized by this miraculous jar of mycelium...then you realize theres a bacterial contamination thats been in there all along, several batches later.

and to be honest, ive never had a multispore bulk run that "didnt fruit" due to "random genetic mix of non-fruiting strains".

in addition, the experience gained in making sterile prints and spore syringes hundreds of times will be invaluable if you ever decide to move on to agar.

sorry for the rant =(


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Offlinewhiterussia
Stranger


Registered: 07/12/07
Posts: 117
Last seen: 15 years, 8 months
Re: all 4 of my jars contamed...wtf [Re: dysphoria]
    #7474131 - 10/01/07 01:55 PM (16 years, 3 months ago)

my Inoculation tek is as you mentioned above with a still air box. I just have no clue why they contam,I though you where supposed to let your grains dry at least a few hours, wont too much moisture in the jar lead to contams? I see growth of myc its just after a week its turned to contams.


--------------------
When you make your peace with authority, you become authority. - Jim Morrison

The most loving parents and relatives commit murder with smiles on their faces. They force us to destroy the person we really are: a subtle kind of murder. - Jim Morrison


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Invisibleorchidfanatic
retiree
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Registered: 08/12/07
Posts: 832
Loc: where the wild things are
Re: all 4 of my jars contamed...wtf [Re: whiterussia]
    #7474157 - 10/01/07 02:03 PM (16 years, 3 months ago)

the nice thing about agar is you can see contamination before you add the
mycelium coated wedge of agar to your grain and it takes 1 cc of a syringe to make many many plates of agar ..its better than LC IMO


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Offlinekrypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 3 months
Re: all 4 of my jars contamed...wtf [Re: whiterussia]
    #7474180 - 10/01/07 02:08 PM (16 years, 3 months ago)

Quote:

whiterussia said:
I though you where supposed to let your grains dry at least a few hours, wont too much moisture in the jar lead to contams?




Where did you read this at?

I'm going by various tek here on the site, particularly Doc's WBS Tek:

http://www.shroomery.org/21/Grain-Spawn-Substrate


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Invisibleshroomerite
Apprentice


Registered: 06/09/06
Posts: 513
Re: all 4 of my jars contamed...wtf *DELETED* [Re: krypto2000]
    #7474656 - 10/01/07 04:43 PM (16 years, 3 months ago)

Post deleted by shroomerite

Reason for deletion: grrrr



--------------------
  "For best results, learn to work with nature rather than against it. Mycelium has an amazing ability to cope with less than optimal conditions, and will often fruit when a grower does everything wrong. However, do everything right and watch your performance go through the roof." RR



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Offlinekrypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 3 months
Re: all 4 of my jars contamed...wtf [Re: shroomerite]
    #7474942 - 10/01/07 06:16 PM (16 years, 3 months ago)

Any thoughts on which is better and why? Long dry vs. short dry that is?


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