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juris1
Esquire



Registered: 08/08/07
Posts: 288
Loc: The South
Last seen: 10 years, 23 days
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Stalled Jars....
#7473140 - 10/01/07 08:40 AM (16 years, 3 months ago) |
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I've got five jars of P.Envy Uncut that have been colonizing for about four weeks now. Three of them are 100% and have been for about a week. The other two have stalled on me. One of them has an area about the size of a half dollar still left to colonize on the very bottom and the other one has most of the bottom and just a little bit up the side. I don't use any kind of incubator. I just put them in my closet where the temps have been staying 78-80F. I do have a layer of verm on the cakes and micropore tape over the holes.
My question is, if I plan on crumbling them and casing them, could I go ahead abd birth those two and just cut off the uncolonized section? I'm gonna go ahead and birth the other three tonight. But if it's ok, i'll go ahead and do the other two as well.
I don't really think it's my setup because I've just had 12 brf/verm B+ cakes to colonize fully. Three of the five P.Envy have colonized. Not sure why these last two have stalled. I drew a line around the uncolonized section last night with a sharpie so I can see if I get any more growth by tonight. I always waited overnight after pc'ing to make sure they were cool enough before incoulating. I followed RR's pf tek method. If anybody has any ideas, suggestions, or advice, I'd greatly appreciate it. Thanks guys!!!
-------------------- "In Vino Veritas" Mycology (from the Greek μύκης, meaning "fungus") is the study of fungi, their genetic and biochemical properties, their taxonomy, and their use to humans as a source for tinder, medicinals (e.g., penicillin), food (e.g., beer, wine, cheese, edible mushrooms) and entheogens, as well as their dangers, such as poisoning or infection.
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impeachme2
AmateurMycologist



Registered: 05/10/07
Posts: 646
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Re: Stalled Jars.... [Re: juris1]
#7473151 - 10/01/07 08:47 AM (16 years, 3 months ago) |
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Ensure you don't have a bacterial contam that is stalling colonization, because if you do, you don't wan't to mix it with the rest of your spawn. If you are positive there is no contam, birth them, removed the uncolonized part, and then use it. Your temps are a little low, and the myc may be simply colonizing inwards instead of downwards right now. IME, they don't always colonize spherically outwards till there is nothing else to colonize, rather they sometimes colonize unidirectionally.
-------------------- tradelist
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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 25 days
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Re: Stalled Jars.... [Re: impeachme2]
#7473217 - 10/01/07 09:25 AM (16 years, 3 months ago) |
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Yup u can simply cut out the uncolinised part and birth ur cakes, or crumble and case (which would produce better results)
I have to disagree that the temps are too low, i have had no problems incubating between 78-82. 84F is an ideal tempurature, 78 i wouldnt think wouldnt be low enough to stop growth, some people here have grown at temps lower.
check that there is no contaminates, other factors for stalled growth is co2 build up, moisture content.
U could try turning ur jar upside down, wheather on not this helps not sure, some say it does and others say it doesnt.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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just me
Friend



Registered: 07/01/07
Posts: 9,745
Loc: IL/MO/FL/TX/HI/OR
Last seen: 4 years, 7 months
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about a week ago, i birthed a cake that appeared to be 100%, but it wasnt, so i went ahead and dunked it, cumbled it, and cased it.
i left the part that wasnt colonized, and broke it up with the rest of the cake. when i put it into the tuperware, the myc spread like wild fire. anything that was uncolonized was colonized the next day. after breaking up the cakes, they are mostly brown in the bag, but the next day it was all white as snow!
i dont know if crumbling the un colonized stuff is wrong, but it definately worked out for me....so far
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  -------------------------------------------------- -pEaCeLoVeGoDbLeSs- "The Downfall of Mankind; is Believing He Has Limitations."
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juris1
Esquire



Registered: 08/08/07
Posts: 288
Loc: The South
Last seen: 10 years, 23 days
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Veda....Thanks for the repy. I agree, I don't think the temps are too low. I've had 12 other 1/2pt jars and 12qt sized jars of wbs all colonize nicely at those temps. I have turned the jars upside down. Actually, they are right now. I will check again tonight and see if there's any new growth. If not, I will just go ahead and birth, and then crumble and case. Also, I don't think there are any contams. I don't see any green trich, or anything else. However, I will isolate them in a separate FC, just in case something is wrong so they won't spread any contams. I don't believe it's moisture because three of the five jars fully colonized. CO2 could be a possibility like you said. I have the verm layer as well as tape over the holes. I'll let ya know how it goes. Thanks again for the help!
-------------------- "In Vino Veritas" Mycology (from the Greek μύκης, meaning "fungus") is the study of fungi, their genetic and biochemical properties, their taxonomy, and their use to humans as a source for tinder, medicinals (e.g., penicillin), food (e.g., beer, wine, cheese, edible mushrooms) and entheogens, as well as their dangers, such as poisoning or infection.
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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 25 days
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Re: Stalled Jars.... [Re: juris1]
#7473488 - 10/01/07 11:09 AM (16 years, 3 months ago) |
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they should smell really strong earthy mushroomy theres no mistaking it. sweet sour or other smell is usually a contam.
good luck with it. I'm sure they will be fine.
take spore prints and get into cloningandlc cultures.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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juris1
Esquire



Registered: 08/08/07
Posts: 288
Loc: The South
Last seen: 10 years, 23 days
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so far, on my first grow, out of 12 brf/verm jars, 12 wbs qt jars, and three B+LC's, I haven't had any contams. The only problem i've had so far is from the five PEnvy jars of which two have stalled. So, things have been good so far i'd say. Especially for a newb. You are right about getting into cloning. I'm ordering some supplies today so I can get into doing that! I just ordered several more strains from Ralphsters today so I can get my collection built up. This is what I have or will have in the next week to be working with...
B+ P.Envy Uncut Brazillian Cambodian F+ Keepers Creeper South African Transki Orissa India Syzygy Dixie Land
I'll be making prints of all these and want to start trading with folks. I already helped out one guy with a B+ print. Shoot me a pm if you'd be interested in anything down the road.
-------------------- "In Vino Veritas" Mycology (from the Greek μύκης, meaning "fungus") is the study of fungi, their genetic and biochemical properties, their taxonomy, and their use to humans as a source for tinder, medicinals (e.g., penicillin), food (e.g., beer, wine, cheese, edible mushrooms) and entheogens, as well as their dangers, such as poisoning or infection.
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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 25 days
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Re: Stalled Jars.... [Re: juris1]
#7474123 - 10/01/07 01:51 PM (16 years, 3 months ago) |
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Thats a nice list. Am waiting on my first 4 brf cakes pinning and hope to get some prints from them.
so far i have
Ecuador Lipa Yai (Thai) Koh Sami Mexican
I still have yet to use the lipa yai and mexican. There all syringes. Think i may start up an LC of each next week.
I unfortunatly had 3 contamed jars out of 4 on the last batch, but i put this down to steam sterilising, now have a PC.
My latest batch all seem fine.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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juris1
Esquire



Registered: 08/08/07
Posts: 288
Loc: The South
Last seen: 10 years, 23 days
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That sounds good. I also went ahead and purchased a samll PC before I got started. After reading so many posts, I decided that's the best way to go. I didn't want to take any chances w/ steam sterilizing and lose a batch after putting so much work in. Probably the best thing i've bought since starting this hobby. However, mine is only a 6qt PC from WalMart. It will hold 5 1/2pt jars and only 2 qt jars. Will be getting a 16qt or larger soon, I hope.
I'd like to try the Mexican and Koh Sami (sp?) at some point. Let me know how you like them or how they perform for you. I'm probably gonna make up some LC's with the syringes listed above. I'm only gonna have one each of the last eight except for Syzygy and Mazatapec, of which i'll have two each of those. I think i'm gonna try the Turbinado LC by Agar. That looks pretty easy. The last LC's I did were the Baby Bottle LC tek. I used Karo and they worked ok, just didn't get a real big ball of mycelium. They were a little larger than the size of a ping pong ball, maybe 1 1/2 times that size. But, enough to make a couple of syringes from each baby bottle. I've got some self sealing rubber stoppers and some of the filters so I can make my own Self-sealing incoulation lids for my lc jars.
ps - I hit ya with a +5 earlier, did ya see it?
-------------------- "In Vino Veritas" Mycology (from the Greek μύκης, meaning "fungus") is the study of fungi, their genetic and biochemical properties, their taxonomy, and their use to humans as a source for tinder, medicinals (e.g., penicillin), food (e.g., beer, wine, cheese, edible mushrooms) and entheogens, as well as their dangers, such as poisoning or infection.
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Vegan
using the searchbutton



Registered: 08/16/07
Posts: 352
Last seen: 14 years, 6 months
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Re: Stalled Jars.... [Re: juris1]
#7474506 - 10/01/07 03:42 PM (16 years, 3 months ago) |
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try the honey for LC you wont regret it
 ive used about 20cc out of this one batch already
-------------------- I came, I saw , I came back
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