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OfflineMakaveli_Clean
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Registered: 07/29/07
Posts: 34
Last seen: 11 years, 6 months
Correctly Innoculating
    #7465213 - 09/28/07 06:28 PM (16 years, 4 months ago)

Ok...

I tried using mycobags a few years back with ZERO growth. I think my main issue was I never allowed any air into the bag (like a dumbass.) After further reading I notice that a lot of people tend to inoculate on the outer part of the substrate.

On my second attempt with mycobags I allowed air to enter the bags so I dont tihnk that will be a problem. However, when I inoculated, I often went deep into the substrate, but was very liberal with the spore solution. I used up to 5ccs per bag. in some bags.

Is there any cause for concern that the bags may stall again because I didn't inoculate on the outer parts of the substrate????

Thanks


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InvisibleCaptainKirk


Registered: 07/15/05
Posts: 2,478
Loc: gone
Re: Correctly Innoculating [Re: Makaveli_Clean]
    #7465225 - 09/28/07 06:32 PM (16 years, 4 months ago)

i may be wrong on this but im pretty sure you need an LC to knock up bulk substrate in a myco bag..somebody that can say for sure feel free to chime in,............not to mention you should use like 60 mls of LC in each bag


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OfflineMakaveli_Clean
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Registered: 07/29/07
Posts: 34
Last seen: 11 years, 6 months
Re: Correctly Innoculating [Re: CaptainKirk]
    #7465255 - 09/28/07 06:43 PM (16 years, 4 months ago)

LC = ?

It says 1-3ccs per bag on sporeworks website.


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InvisibleCaptainKirk


Registered: 07/15/05
Posts: 2,478
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Re: Correctly Innoculating [Re: Makaveli_Clean]
    #7465274 - 09/28/07 06:53 PM (16 years, 4 months ago)

ohhh im guessing its not poo in your bags..you maybe alright in that case,if its just grains in there spore should work fine, i thought you had bulk substrate (poo )


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Invisiblejeetered
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Registered: 07/07/06
Posts: 3,055
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Re: Correctly Innoculating [Re: CaptainKirk]
    #7465285 - 09/28/07 06:57 PM (16 years, 4 months ago)

inoculating rye bags, w/o LC is difficult, takes a long time, and is just unordinary, any bulk spawn medium, needs to be inoculated with a liquid culture for speed of colonization.

During colonization you want co2, not o2.


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