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Invisiblebudmanman
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Registered: 02/07/07
Posts: 17,975
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Atempting open air clone to lc possible?
    #7462198 - 09/27/07 09:15 PM (16 years, 4 months ago)

So I was thinking of pcing a syringe with some water in it and then shoving it through the best mush I got. Before shoving it through I would wipe both sides well with alcohol soaked tow let.

Then Inoculate my LC with it.

I have always done work in open air and thought maybe this could be done maybe no?


--------------------
Everything I have ever said is total bogus bs I am full of crud therefore everything I say should never be taken literal.

And I am mentally unstable.


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Offlinepazzy
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Registered: 08/24/07
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Re: Atempting open air clone to lc possible? [Re: budmanman]
    #7462224 - 09/27/07 09:21 PM (16 years, 4 months ago)

It could work. Iodine would be better then the alchohol. Just work quickly in a clean room and you have a pretty good chance.


--------------------
Are you happy for a miracle?

:yinyang2:

Absolutely no source checks/discussions.


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OfflineeVenom
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Re: Atempting open air clone to lc possible? [Re: pazzy]
    #7462409 - 09/27/07 10:20 PM (16 years, 4 months ago)

IT works but I recommend more sterile methods:Here!!

and iodine is better that alcohol but H2O2 is really good and even if it shocks the outside mycelium the inside will be unharmed!!

try it! and good luck!


--------------------
eVenom

"Some of us like a nice buzz, while others want to climb a screaming volcano naked to meet god."
RR


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OfflineRogerRabbitM
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Re: Atempting open air clone to lc possible? [Re: eVenom]
    #7463213 - 09/28/07 06:22 AM (16 years, 4 months ago)

Possible, but not probable success. Use a glovebox.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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Offlinerizingfire
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Re: Atempting open air clone to lc possible? [Re: RogerRabbit]
    #7463258 - 09/28/07 06:45 AM (16 years, 4 months ago)

So bored, basically if you waste 20 cakes you will trip, how many shrooms and GOOD trips could you have had? This thread is just stupid. Psilocin is water soluable so how is it sinking to the bottom? Full of shit! That is all you are. There isn't enough psilocin in my whole FC doing it this way. And you said chuck the cakes cuz the psilocin is gone from the cake? The ammount of psilocin in a developed mushroom is far more than the cake ever had...so next time you and your stoner friends sit around making up shit, don't post it as fact...you are a tool and I can only imagine how many will try this and get sick from contamns...and bongwater is full of tar and bacteria...there is some thc but not enough to be worth it. I would evaporate it before I would ever drink it...personally it gets tossed


--------------------
aka NHMI


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Offlinehosedragger
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Re: Atempting open air clone to lc possible? [Re: rizingfire]
    #7463288 - 09/28/07 07:00 AM (16 years, 4 months ago)

Where on earth did that last post come from???


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OfflineMycodood
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Re: Atempting open air clone to lc possible? [Re: hosedragger]
    #7463366 - 09/28/07 07:57 AM (16 years, 4 months ago)

I tried this Biopsy method last night and it did not work.
No tissue entered the needle. So I went for an open air
LC inoc. I have done open air and failed before but I really
didn't have a choice since all the materials were out
on my counter and I wanted to move fast. I had a PE specimen that
was the size of a babies arm so I had to give it a shot.

What I did was swab the outside of the shroom with Alcohol,
I sliced it open. Cust a piece of the tissue from the
inside and opened the jar top just enough to drop in the specimen.
I will know in a bout 2 or 3 days whether iot is screwed or not.


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OfflineMFGFA37
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Re: Atempting open air clone to lc possible? [Re: Mycodood]
    #7463579 - 09/28/07 09:46 AM (16 years, 4 months ago)

Babies arm eh? I would love to see a picture of that! Keep us updated, I hope you have success!


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There is no try, only do. -Yoda-

Take no thought for tomorrow, tomorrow shall take thought for itself. Sufficient for one day are the thoughts/worries for that day. Any thought past this is useless. -Ole Jesus-


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OfflineanarchOi
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Re: Atempting open air clone to lc possible? [Re: MFGFA37]
    #7463616 - 09/28/07 09:59 AM (16 years, 4 months ago)

i couldn't get the stick the syringe through the stem thing to work

i was going to try this same thing in open air, but i ended up not being able to get any stem pieces into the needle, it seemed to just make a hole around the needle, and i use a pretty big needle

i ended up cutting a string out of the center with a flamed knife


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OfflineMycodood
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Re: Atempting open air clone to lc possible? [Re: budmanman]
    #7463620 - 09/28/07 10:00 AM (16 years, 4 months ago)

Ha Ha, Yeah I was lazy so I didn't snap any but I have
3 more that are going to be ready to pick tomorrow so I will
try and get one snapped. Unreal.
I was getting some huge fruits with an 80/20 coir/coffee mix and
this time around I did an 80/10/10 coir/coffee/cpoo and they are
so much bigger.


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Invisiblebudmanman
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Registered: 02/07/07
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Re: Atempting open air clone to lc possible? [Re: Mycodood]
    #7465202 - 09/28/07 06:20 PM (16 years, 4 months ago)

I stuck my needle through it and then stuck it into the lc and when i tryed to squirt it I felt the peace shoot out, however I can not find it in the LC i did feel it and know its in there, maybe this is your guyses problem?


--------------------
Everything I have ever said is total bogus bs I am full of crud therefore everything I say should never be taken literal.

And I am mentally unstable.


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Invisibledeucedbi9
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Registered: 10/24/06
Posts: 4,594
Loc: UK
Re: Atempting open air clone to lc possible? [Re: anarchOi]
    #7466373 - 09/29/07 04:29 AM (16 years, 4 months ago)

Quote:

anarchOi said:
i couldn't get the stick the syringe through the stem thing to work

i was going to try this same thing in open air, but i ended up not being able to get any stem pieces into the needle, it seemed to just make a hole around the needle, and i use a pretty big needle

i ended up cutting a string out of the center with a flamed knife




anarch0i
"... i ended up not being able to get any stem pieces into the needle"

if you hold an old needle with pliers and hack-saw the the tip of at an angle you should be left with a sharp burr on the inside that will help to snag a piece of mateial.
i take a sample by breaking the stem in half and pushing the needle up the stem 2-3 times with a twisting motion,hopefully snagging a piece of mushroom each time.
you may find you need to "pump" the syringe with the liquid medium a few times to dislodge the pieces.
i had two out of three clone attempts work this way, in open air.

btw budman.one time i could see 3 distinct pieces in the lc,after a week or so there were 4 separate growths,one much smaller than the rest.i'm guessing the 4th growth was initially microscopic.


--------------------
whether low pressure sucks or high pressure blows...
it's a bugger to cycle in.

even though I'm feeling good
Something tells me I'd better activate my prayer capsule


Edited by deucedbi9 (09/29/07 04:35 AM)


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Offlinethedoc8
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Re: Atempting open air clone to lc possible? [Re: Mycodood]
    #7466471 - 09/29/07 06:41 AM (16 years, 4 months ago)

Quote:

Mycodood said:
I tried this Biopsy method last night and it did not work.
No tissue entered the needle. So I went for an open air
LC inoc. I have done open air and failed before but I really
didn't have a choice since all the materials were out
on my counter and I wanted to move fast. I had a PE specimen that
was the size of a babies arm so I had to give it a shot.

What I did was swab the outside of the shroom with Alcohol,
I sliced it open. Cust a piece of the tissue from the
inside and opened the jar top just enough to drop in the specimen.
I will know in a bout 2 or 3 days whether iot is screwed or not.




I could not get a piece in the syringe, so I open air cleanly cut a small piece and put in the jar under a flo hood. Not the best but I have got good lc 3 out of 3 times.


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Invisiblebudmanman
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Registered: 02/07/07
Posts: 17,975
Loc: PNW
Re: Atempting open air clone to lc possible? [Re: thedoc8]
    #7470317 - 09/30/07 12:37 PM (16 years, 4 months ago)

Well its been a few days and I see no growth, but using a lamp inside I can see a peace of shroom in there.

Will peace start to grow?


--------------------
Everything I have ever said is total bogus bs I am full of crud therefore everything I say should never be taken literal.

And I am mentally unstable.


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OfflineMycodood
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Registered: 05/22/07
Posts: 277
Loc: USA
Last seen: 2 years, 11 months
Re: Atempting open air clone to lc possible? [Re: budmanman]
    #7470461 - 09/30/07 01:23 PM (16 years, 4 months ago)

Yeah Mine didn't work. Contammed within 3 days. I need a glove box..


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OfflineanarchOi
Ellenalien is fat.
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Registered: 08/06/07
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Re: Atempting open air clone to lc possible? [Re: Mycodood]
    #7473083 - 10/01/07 07:55 AM (16 years, 3 months ago)

eh well
i pulled off slicing a string from the stem with a flamed and OPENING (yes insane i know) the jar to put it into it

the LC is slow and i expect a failure
but the agar is going nicely


--------------------


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OfflineMycodood
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Registered: 05/22/07
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Re: Atempting open air clone to lc possible? [Re: deucedbi9]
    #7473172 - 10/01/07 08:55 AM (16 years, 3 months ago)

Yeah I didn't feel a little piece of anything but IDK....
maybe it was there but I ended up fucking it up.
I am going to try this method again though.


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