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budmanman
OTD Masterbater



Registered: 02/07/07
Posts: 17,975
Loc: PNW
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Atempting open air clone to lc possible?
#7462198 - 09/27/07 09:15 PM (16 years, 4 months ago) |
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So I was thinking of pcing a syringe with some water in it and then shoving it through the best mush I got. Before shoving it through I would wipe both sides well with alcohol soaked tow let.
Then Inoculate my LC with it.
I have always done work in open air and thought maybe this could be done maybe no?
-------------------- Everything I have ever said is total bogus bs I am full of crud therefore everything I say should never be taken literal. And I am mentally unstable.
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pazzy
Always Hardcore


Registered: 08/24/07
Posts: 1,360
Last seen: 13 years, 2 months
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Re: Atempting open air clone to lc possible? [Re: budmanman]
#7462224 - 09/27/07 09:21 PM (16 years, 4 months ago) |
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It could work. Iodine would be better then the alchohol. Just work quickly in a clean room and you have a pretty good chance.
-------------------- Are you happy for a miracle?
Absolutely no source checks/discussions.
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eVenom
DRIFT on toBATTLE



Registered: 08/25/07
Posts: 869
Loc: Florida
Last seen: 4 months, 13 days
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Re: Atempting open air clone to lc possible? [Re: pazzy]
#7462409 - 09/27/07 10:20 PM (16 years, 4 months ago) |
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IT works but I recommend more sterile methods:Here!!
and iodine is better that alcohol but H2O2 is really good and even if it shocks the outside mycelium the inside will be unharmed!!
try it! and good luck!
-------------------- eVenom "Some of us like a nice buzz, while others want to climb a screaming volcano naked to meet god." RR
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: Atempting open air clone to lc possible? [Re: eVenom]
#7463213 - 09/28/07 06:22 AM (16 years, 4 months ago) |
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Possible, but not probable success. Use a glovebox. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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rizingfire
Mycoticus psychoticus




Registered: 09/10/07
Posts: 875
Loc: North-east USm'f'nA
Last seen: 4 months, 11 days
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Re: Atempting open air clone to lc possible? [Re: RogerRabbit]
#7463258 - 09/28/07 06:45 AM (16 years, 4 months ago) |
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So bored, basically if you waste 20 cakes you will trip, how many shrooms and GOOD trips could you have had? This thread is just stupid. Psilocin is water soluable so how is it sinking to the bottom? Full of shit! That is all you are. There isn't enough psilocin in my whole FC doing it this way. And you said chuck the cakes cuz the psilocin is gone from the cake? The ammount of psilocin in a developed mushroom is far more than the cake ever had...so next time you and your stoner friends sit around making up shit, don't post it as fact...you are a tool and I can only imagine how many will try this and get sick from contamns...and bongwater is full of tar and bacteria...there is some thc but not enough to be worth it. I would evaporate it before I would ever drink it...personally it gets tossed
-------------------- aka NHMI
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hosedragger
Stranger


Registered: 06/13/07
Posts: 50
Last seen: 16 years, 1 month
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Re: Atempting open air clone to lc possible? [Re: rizingfire]
#7463288 - 09/28/07 07:00 AM (16 years, 4 months ago) |
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Where on earth did that last post come from???
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Mycodood



Registered: 05/22/07
Posts: 277
Loc: USA
Last seen: 2 years, 11 months
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Re: Atempting open air clone to lc possible? [Re: hosedragger]
#7463366 - 09/28/07 07:57 AM (16 years, 4 months ago) |
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I tried this Biopsy method last night and it did not work. No tissue entered the needle. So I went for an open air LC inoc. I have done open air and failed before but I really didn't have a choice since all the materials were out on my counter and I wanted to move fast. I had a PE specimen that was the size of a babies arm so I had to give it a shot.
What I did was swab the outside of the shroom with Alcohol, I sliced it open. Cust a piece of the tissue from the inside and opened the jar top just enough to drop in the specimen. I will know in a bout 2 or 3 days whether iot is screwed or not.
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MFGFA37
Me For God ForAll



Registered: 09/08/07
Posts: 114
Loc: Cheese and Beer
Last seen: 12 years, 1 month
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Re: Atempting open air clone to lc possible? [Re: Mycodood]
#7463579 - 09/28/07 09:46 AM (16 years, 4 months ago) |
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Babies arm eh? I would love to see a picture of that! Keep us updated, I hope you have success!
-------------------- There is no try, only do. -Yoda- Take no thought for tomorrow, tomorrow shall take thought for itself. Sufficient for one day are the thoughts/worries for that day. Any thought past this is useless. -Ole Jesus-
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anarchOi
Ellenalien is fat.



Registered: 08/06/07
Posts: 2,293
Loc: ASE
Last seen: 12 years, 11 months
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Re: Atempting open air clone to lc possible? [Re: MFGFA37]
#7463616 - 09/28/07 09:59 AM (16 years, 4 months ago) |
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i couldn't get the stick the syringe through the stem thing to work
i was going to try this same thing in open air, but i ended up not being able to get any stem pieces into the needle, it seemed to just make a hole around the needle, and i use a pretty big needle
i ended up cutting a string out of the center with a flamed knife
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Mycodood



Registered: 05/22/07
Posts: 277
Loc: USA
Last seen: 2 years, 11 months
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Re: Atempting open air clone to lc possible? [Re: budmanman]
#7463620 - 09/28/07 10:00 AM (16 years, 4 months ago) |
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Ha Ha, Yeah I was lazy so I didn't snap any but I have 3 more that are going to be ready to pick tomorrow so I will try and get one snapped. Unreal. I was getting some huge fruits with an 80/20 coir/coffee mix and this time around I did an 80/10/10 coir/coffee/cpoo and they are so much bigger.
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budmanman
OTD Masterbater



Registered: 02/07/07
Posts: 17,975
Loc: PNW
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Re: Atempting open air clone to lc possible? [Re: Mycodood]
#7465202 - 09/28/07 06:20 PM (16 years, 4 months ago) |
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I stuck my needle through it and then stuck it into the lc and when i tryed to squirt it I felt the peace shoot out, however I can not find it in the LC i did feel it and know its in there, maybe this is your guyses problem?
-------------------- Everything I have ever said is total bogus bs I am full of crud therefore everything I say should never be taken literal. And I am mentally unstable.
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deucedbi9
Stranger

Registered: 10/24/06
Posts: 4,594
Loc: UK
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Re: Atempting open air clone to lc possible? [Re: anarchOi]
#7466373 - 09/29/07 04:29 AM (16 years, 4 months ago) |
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Quote:
anarchOi said: i couldn't get the stick the syringe through the stem thing to work
i was going to try this same thing in open air, but i ended up not being able to get any stem pieces into the needle, it seemed to just make a hole around the needle, and i use a pretty big needle
i ended up cutting a string out of the center with a flamed knife
anarch0i "... i ended up not being able to get any stem pieces into the needle"
if you hold an old needle with pliers and hack-saw the the tip of at an angle you should be left with a sharp burr on the inside that will help to snag a piece of mateial. i take a sample by breaking the stem in half and pushing the needle up the stem 2-3 times with a twisting motion,hopefully snagging a piece of mushroom each time. you may find you need to "pump" the syringe with the liquid medium a few times to dislodge the pieces. i had two out of three clone attempts work this way, in open air.
btw budman.one time i could see 3 distinct pieces in the lc,after a week or so there were 4 separate growths,one much smaller than the rest.i'm guessing the 4th growth was initially microscopic.
-------------------- whether low pressure sucks or high pressure blows... it's a bugger to cycle in. even though I'm feeling good Something tells me I'd better activate my prayer capsule
Edited by deucedbi9 (09/29/07 04:35 AM)
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thedoc8
TooMuch


Registered: 05/18/07
Posts: 442
Last seen: 11 years, 3 months
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Re: Atempting open air clone to lc possible? [Re: Mycodood]
#7466471 - 09/29/07 06:41 AM (16 years, 4 months ago) |
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Quote:
Mycodood said: I tried this Biopsy method last night and it did not work. No tissue entered the needle. So I went for an open air LC inoc. I have done open air and failed before but I really didn't have a choice since all the materials were out on my counter and I wanted to move fast. I had a PE specimen that was the size of a babies arm so I had to give it a shot.
What I did was swab the outside of the shroom with Alcohol, I sliced it open. Cust a piece of the tissue from the inside and opened the jar top just enough to drop in the specimen. I will know in a bout 2 or 3 days whether iot is screwed or not.
I could not get a piece in the syringe, so I open air cleanly cut a small piece and put in the jar under a flo hood. Not the best but I have got good lc 3 out of 3 times.
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budmanman
OTD Masterbater



Registered: 02/07/07
Posts: 17,975
Loc: PNW
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Re: Atempting open air clone to lc possible? [Re: thedoc8]
#7470317 - 09/30/07 12:37 PM (16 years, 4 months ago) |
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Well its been a few days and I see no growth, but using a lamp inside I can see a peace of shroom in there.
Will peace start to grow?
-------------------- Everything I have ever said is total bogus bs I am full of crud therefore everything I say should never be taken literal. And I am mentally unstable.
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Mycodood



Registered: 05/22/07
Posts: 277
Loc: USA
Last seen: 2 years, 11 months
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Re: Atempting open air clone to lc possible? [Re: budmanman]
#7470461 - 09/30/07 01:23 PM (16 years, 4 months ago) |
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Yeah Mine didn't work. Contammed within 3 days. I need a glove box..
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anarchOi
Ellenalien is fat.



Registered: 08/06/07
Posts: 2,293
Loc: ASE
Last seen: 12 years, 11 months
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Re: Atempting open air clone to lc possible? [Re: Mycodood]
#7473083 - 10/01/07 07:55 AM (16 years, 3 months ago) |
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eh well i pulled off slicing a string from the stem with a flamed and OPENING (yes insane i know) the jar to put it into it
the LC is slow and i expect a failure but the agar is going nicely
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Mycodood



Registered: 05/22/07
Posts: 277
Loc: USA
Last seen: 2 years, 11 months
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Re: Atempting open air clone to lc possible? [Re: deucedbi9]
#7473172 - 10/01/07 08:55 AM (16 years, 3 months ago) |
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Yeah I didn't feel a little piece of anything but IDK.... maybe it was there but I ended up fucking it up. I am going to try this method again though.
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