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Shop: Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures, Substrate Bags

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Offlinekatfish
Nooblit
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Registered: 07/22/06
Posts: 592
Loc: Louisiana
Last seen: 15 years, 11 months
liquid culture prob
    #7460624 - 09/27/07 02:36 PM (16 years, 4 months ago)

Ok so i have a liquid culture that I made with some karo and water solution, I actually just ordered the premade stuff from a myco dealer. I injected some multispores and walla, i have lots of myclieum floating in the jar. Now I have a self healing injector site on the top of the lid so I take a sterile syringe and tilt the jar until the needle reaches the water and extract.

Heres the problem,

I have taken from it twice, to try to inoculate 4 mycobags that I tried with some spores but they didn't take.
Now when I try to get some of the liquid culture out the jar the neddle doesn't reach the water,


could i take some clean water and add to the liquid culture? or do I have to get a big needle or move the LC into a smaller bottle( which I am assuming will take a glovebox.)

I just don't want to contaminate it or if it is possible, water the LC down too much.


--------------------
08/2/07 Inoculated mycobag with Brazilian, 3cc
08/08/07 First mycelium show
09/03/07 Brazilian still colonizing, Three Mycobags inoculated with Koi Somui
10/11/07 2 Pint PESA 100% colonized. 1 pint Amazon 90% colonized
10/12/07 Storage bin perite FC constructed
10/28/07 Havested my First ever flush, of PESA!


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OfflineFraggin
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Registered: 01/05/05
Posts: 8,707
Last seen: 8 years, 3 days
Re: liquid culture prob [Re: katfish]
    #7460640 - 09/27/07 02:39 PM (16 years, 4 months ago)

Why not tip the jar to the side?


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Offlinekatfish
Nooblit
Male User Gallery


Registered: 07/22/06
Posts: 592
Loc: Louisiana
Last seen: 15 years, 11 months
Re: liquid culture prob [Re: Fraggin]
    #7460664 - 09/27/07 02:44 PM (16 years, 4 months ago)

it is too low and I am afriad of getting the filter patch on the top wet.

check em out

http://cgi.ebay.com/ws/eBayISAPI.dll?ViewItem&rd=1&item=270160342139&ssPageName=STRK:MEWA:IT&ih=017


they work awesome and are sterile.


--------------------
08/2/07 Inoculated mycobag with Brazilian, 3cc
08/08/07 First mycelium show
09/03/07 Brazilian still colonizing, Three Mycobags inoculated with Koi Somui
10/11/07 2 Pint PESA 100% colonized. 1 pint Amazon 90% colonized
10/12/07 Storage bin perite FC constructed
10/28/07 Havested my First ever flush, of PESA!


Extras: Filter Print Post Top
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Shop: Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures, Substrate Bags


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