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OfflineShroomDoom
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Registered: 06/08/04
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why use LC?
    #7454918 - 09/26/07 10:41 AM (14 years, 28 days ago)

i see a lot of the new grow logs and it seems like LC is so popular right now. why? what ever happened to just good ol fashion spores dripping onto the substrate? is it because you can directly inject LC to a bulk substrate?


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InvisibleCaptainKirk


Registered: 07/15/05
Posts: 2,478
Loc: gone
Re: why use LC? [Re: ShroomDoom]
    #7454928 - 09/26/07 10:44 AM (14 years, 28 days ago)

lc is much quicker as the myc is already growing ,no need to wait on the spores to do their thing, and for like you said its the only way to knock up bulk subs


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OfflineWronguy

Registered: 03/05/05
Posts: 4,450
Last seen: 10 years, 3 months
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Re: why use LC? [Re: CaptainKirk]
    #7455020 - 09/26/07 11:17 AM (14 years, 28 days ago)

Correct. Besides using spawn for bulk substrate inoculations, liquid cultures are great. The amount of time can also be drastically reduced when using an LC. It all depends on the amount used though.

Good to see you around Captain!


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Offlinewutang
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Registered: 06/29/07
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Re: why use LC? [Re: Wronguy]
    #7455141 - 09/26/07 12:18 PM (14 years, 28 days ago)

yeah lc speeds up the growth


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OfflineTheDudeAbides
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Registered: 10/23/01
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Re: why use LC? [Re: wutang]
    #7455148 - 09/26/07 12:19 PM (14 years, 28 days ago)

LC?

Ridiculous question, I know...


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outputrotation said:
x-com and unsolved mysteries are the only things that have ever made me truly scared


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OfflineanarchOi
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Registered: 08/06/07
Posts: 2,293
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Re: why use LC? [Re: TheDudeAbides]
    #7455208 - 09/26/07 12:38 PM (14 years, 28 days ago)

live culture, or liquid culture
whichever you prefer

you can even make cakes with cloned LC >.>


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Offlinegmuralid
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Registered: 08/05/07
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Loc: India
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Re: why use LC? [Re: anarchOi]
    #7455224 - 09/26/07 12:41 PM (14 years, 28 days ago)

Holy shit. You have Ganesh as your avatar! The ganpati festival is just getting over here, I dunno if you know what that is.


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Wilderness. It defines me.


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Offlinegmuralid
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Re: why use LC? [Re: gmuralid]
    #7455238 - 09/26/07 12:44 PM (14 years, 28 days ago)

And yeah I just started using LC and the honey tek has been awesome! It takes me 2 days to see anything, but after that the myc is just explosive. And the jars I inoculated showed myc growth the next day!


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OfflineVegan
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Registered: 08/16/07
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Re: why use LC? [Re: TheDudeAbides]
    #7455246 - 09/26/07 12:45 PM (14 years, 28 days ago)

I started using LC (liquid culture) for the speed thing but now I'm looking at it for the cloning properties..

heres a pic of 2 jars one with spores
the other with LC
same innoc day



also in my experiments with WBS the longer it takes to colonize a jar the more chances of contams setting in,..
cause of this I wont do WBS with spores again,, brf cakes yes but no more wbs..I lose about half with spores ..perhaps its my tech but either way..


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Offlineaspore
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Registered: 07/10/07
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Re: why use LC? [Re: Vegan]
    #7455263 - 09/26/07 12:51 PM (14 years, 28 days ago)

Yes, liquid cultures are just mycelium suspeneded in a nutritive solution. Growing in it. It speeds up your colinization times a lot. Also, it's easy to generate a larger portion of LC's than spore solutions.

Also, you can make a clone mushroom into LC, giving you the genetics of that mushroom.

!aspore


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Offlinemutex
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Registered: 08/20/07
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Re: why use LC? [Re: aspore]
    #7455912 - 09/26/07 04:15 PM (14 years, 28 days ago)

I use LC as insurance. I'm just starting out so I'm trying different things to see what works for me and what doesn't. Cakes, different grains, LC's with diff ingredients, etc. Ive fucked up a few things (ok probably more than a few) here and there but if the LC gets low you just go make more. Without LC I think I would of spent alot of money on spore syringes just this month!

One thing I don't like about LC is you really don't know if its good, esp if your just starting out and your not sure what its supposed to look like. I'm trying mycelium syringes (water from colonized grains) now. I think it makes more sense for what I'm using it for.


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Offlinewutang
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Registered: 06/29/07
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Re: why use LC? [Re: Vegan]
    #7455934 - 09/26/07 04:24 PM (14 years, 28 days ago)

Quote:

Vegan said:
I started using LC (liquid culture) for the speed thing but now I'm looking at it for the cloning properties..

heres a pic of 2 jars one with spores
the other with LC
same innoc day



also in my experiments with WBS the longer it takes to colonize a jar the more chances of contams setting in,..
cause of this I wont do WBS with spores again,, brf cakes yes but no more wbs..I lose about half with spores ..perhaps its my tech but either way..




????????????
what day was it shot and what date was the pic taken?


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OfflineDigital Reality
Strangers in the dark
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Registered: 05/01/07
Posts: 187
Last seen: 5 years, 9 months
Re: why use LC? [Re: ShroomDoom]
    #7456047 - 09/26/07 05:02 PM (14 years, 28 days ago)

The biggest benefit with LC is not speed. It’s a great thing to have but I consider an insurance of good genetics as stated before an entire factor I can’t over look. And for the people that don’t understand what genetics are good for in a mushroom, look at it like this.

Multi spore inoculations are nothing more than a role of the dice. You are correct in saying that all of your spores are the same strain. But not all of them are equal. You have weak spores, strong, spore, and piece of shit spores that do nothing. Basicly what im saying is not every spore in you syringe will germinate, or grow mycelieum, or even help produce a mushroom. That’s one reason some people have great pin sets and picture perfect flushes and others don’t. The ones that don’t could be contributed to the fact that one side of you cake had kick ass motivated spores while the other side is full of a bunch of lazy bastards that might make a mushroom or two.

Now to avoid this and help ensure a better chance and gnarly ass shrooms on your next grow is starting a LC from a clone. There was a tech I saw a while back for cloning to LC and was just stupid simple.

You simply take your best shroom out of your grow. Put it in you glove box. Wipe it down with alcohol and immediately take a sterile syring and pierce through the shroom so you have a chunk in the needle. Then you simply inject that into you LC mix and BAM. In a week or two you have LC with genetics of workers. So your chances of a nice pin set, with shrooms that have the same characteristics you cloned from.

This is the tech for cloning that im referring to.
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/5874305/page/0/fpart/1/vc/1/nt/4

And this is my preferred way to make LC. Its free if you get your sugar at star bucks and turns out damn near clear so you can see what’s going on inside the jar.
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/6378527/page/0/fpart/1/vc/1

Just my two cents based on the reading ive done.
  :penguinmonkey:


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