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Offlinevm711
Stranger
Registered: 09/16/07
Posts: 49
Last seen: 16 years, 1 month
Re: Can I make more spores from spores? [Re: Too Vanilla]
    #7463354 - 09/28/07 07:48 AM (16 years, 4 months ago)

Creamcorn's LC cloning tek seems to be the simplest for me to follow, I was asking if I could use the spores instead of cloning tissue for this particular guide.


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OfflineToo Vanilla
The Un-Negro
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Registered: 09/19/07
Posts: 79
Loc: Hickory, NC
Last seen: 16 years, 3 months
Re: Can I make more spores from spores? [Re: vm711]
    #7463368 - 09/28/07 07:57 AM (16 years, 4 months ago)

I don't think so, because it appears this cloning produces mycelium rather than spores (which I thought is what you wanted to do).

If I'm way off, let me know. I'm a n--b and I'm learning.


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Offlinevm711
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Registered: 09/16/07
Posts: 49
Last seen: 16 years, 1 month
Re: Can I make more spores from spores? [Re: Too Vanilla]
    #7463370 - 09/28/07 07:59 AM (16 years, 4 months ago)

The thread title is a bit misleading, I didn't realize there wasn't actually a way to make spores from spores, and producing a mycelium culture will actually make the process faster. My only question is if I can use this tek and instead of using the tissue from a shroom, use a couple ml of spore solution.


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OfflineToo Vanilla
The Un-Negro
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Registered: 09/19/07
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Loc: Hickory, NC
Last seen: 16 years, 3 months
Re: Can I make more spores from spores? [Re: vm711]
    #7463379 - 09/28/07 08:03 AM (16 years, 4 months ago)

Def.

Its just a little harder to keep things sanitary when you transfer from the honey/karo jar to the substrate jar (IMO).


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Offlinevm711
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Registered: 09/16/07
Posts: 49
Last seen: 16 years, 1 month
Re: Can I make more spores from spores? [Re: Too Vanilla]
    #7463392 - 09/28/07 08:10 AM (16 years, 4 months ago)

So in this case I need to get a half pint jar and use karo and distilled water, drill a hole in the lid and put micropore tape over it, sterilize the whole deal, let it cool, inject the jar with my spore syringe a couple ml, then stick another peice of micropore tape over it the hole I just made. This sound right?

When it comes time to inoculate my jars with the new LC, I draw culture with a sterile syringe and inject just as much as I would from a spore syringe into each jar? And it's ok to be innoculating jars with this karo solution, or must I somehow seperate the nutrients and mycelium?

sorry about all the questions, and I greatly appreciate your guidance


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Invisiblesimplemachine
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Registered: 09/14/03
Posts: 1,981
Re: Can I make more spores from spores? [Re: vm711]
    #7463413 - 09/28/07 08:20 AM (16 years, 4 months ago)

Quote:

vm711 said:
Great! Can I follow this tek and use the spores to inoculate the LC jar? I only have spores in a syringe, not mushrooms to clone.




Yep, it'll work with spores as well.


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