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Offlinevm711
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Registered: 09/16/07
Posts: 49
Last seen: 16 years, 1 month
Can I make more spores from spores?
    #7455832 - 09/26/07 01:47 PM (16 years, 4 months ago)

I have a dillema - I have 24 half pint jars and only one 10ml syringe of B+ to inoculate them with. I've read that I can make a spore print and whatnot but I have no mushrooms to work with, only spores from the syringe. Can I make more spores, enough to inoculate all the jars easily?
thanks


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Invisibleblood4blood
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Registered: 04/25/07
Posts: 6,029
Loc: The Valley
Re: Can I make more spores from spores? [Re: vm711]
    #7455842 - 09/26/07 01:50 PM (16 years, 4 months ago)

you could make an lc...


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Offlineveda_sticks
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Re: Can I make more spores from spores? [Re: blood4blood]
    #7455850 - 09/26/07 01:55 PM (16 years, 4 months ago)

1 spore syringe usually makes 10 jars if u r sparse with ur solution, ie 1cc per jar.

If u make up an lc, then u could make up several syringes worth. Ur spore syringe will make lots of lc culture, and i mean alot, which would then go on to making a ton of jars.

Im not exactly sure on the numbers, but lets say a half pint culture, i could imagine that would make a good 10 syringes. out of 1 syringe u would get at least 10 lc cultures.

ofcoarse u would have an extra week or so to grow ur cultures, but once they are grown, inoced jars will grow pretty fast. my first lc fully colinised in about a week (as there the spores had already germinated)

once u have ur mshrooms fruited, then spore print away.

look into cloning aswell.

If u research well and understand everything, then once u get goin u should never haver to buy spores again, only if u wwant aneother strain


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


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OfflineDigital Reality
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Re: Can I make more spores from spores? [Re: vm711]
    #7455851 - 09/26/07 01:55 PM (16 years, 4 months ago)

Nope, the only way to get more spores it from a shroom. You could make a Liquid Culture with your spores. You just use a few cc's of spore solution and knock up a quart jar of LC and you have a shit ton of Solution to knock up your jars with. Try this tech. It works effin great and the sugar is free at star bucks. Doesn't get much easier than that.


http://www.shroomery.org/forums/showflat.php/Cat/0/Number/6378527/page/0/fpart/1/vc/1


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Offlinehighc
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Re: Can I make more spores from spores? [Re: blood4blood]
    #7455853 - 09/26/07 01:55 PM (16 years, 4 months ago)

Quote:

blood4blood said:
you could make an lc...




:smile:
this would be your best bet or buy another syringe. or what i would do in your position to late now but you could have prepared 3 rye jars and used them to inoculate your pints later.


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Offlinejuris1
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Re: Can I make more spores from spores? [Re: vm711]
    #7455862 - 09/26/07 01:57 PM (16 years, 4 months ago)

Liquid Culture The Easy Way
https://www.shroomery.org/forums/showflat.php/Number/4141612#Post4141612

This is Agar's baby bottle tek. It's very easy to use. I did three this way when I started my first grow about give weeks ago and they all did great. If you do this, you should have more than enough LC to noc up your jars or whatever else you feel like doing! Give it a try. It may take you up to two weeks to get a good amount of myc in your bottle. I suppose you coud go ahead and suck some of the myc out early, but I'd wait for it to finish growing and then draw it out with a syringe. GL


--------------------
"In Vino Veritas"

Mycology (from the Greek μύκης, meaning "fungus") is the study of fungi, their genetic and biochemical properties, their taxonomy, and their use to humans as a source for tinder, medicinals (e.g., penicillin), food (e.g., beer, wine, cheese, edible mushrooms) and entheogens, as well as their dangers, such as poisoning or infection.



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Offlinejuris1
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Re: Can I make more spores from spores? [Re: juris1]
    #7455886 - 09/26/07 02:05 PM (16 years, 4 months ago)

I didn't see the other posts before me when I put my up. Guess they were all being done at the same time. I looked at the link above and it looks great. I'd deff recommend giving that a try first before doing the baby bottle tek. Looks easier! I'm gonna try it tonight. Good luck


--------------------
"In Vino Veritas"

Mycology (from the Greek μύκης, meaning "fungus") is the study of fungi, their genetic and biochemical properties, their taxonomy, and their use to humans as a source for tinder, medicinals (e.g., penicillin), food (e.g., beer, wine, cheese, edible mushrooms) and entheogens, as well as their dangers, such as poisoning or infection.



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Offlinetonyperez420
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Re: Can I make more spores from spores? [Re: juris1]
    #7455896 - 09/26/07 02:09 PM (16 years, 4 months ago)

doesnt the LC need air exchange

how is it exchanged in agar's baby bottle tek?


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Invisibleblood4blood
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Registered: 04/25/07
Posts: 6,029
Loc: The Valley
Re: Can I make more spores from spores? [Re: tonyperez420]
    #7455908 - 09/26/07 02:14 PM (16 years, 4 months ago)

theres enough room in the bottle. or you could put a filter syringe in it..


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OfflineDigital Reality
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Registered: 05/01/07
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Re: Can I make more spores from spores? [Re: blood4blood]
    #7455989 - 09/26/07 02:44 PM (16 years, 4 months ago)

Or every few days just take a syringe and hold the point in the flame until its red hot and then slowly suck in air while keeping it in the flame. Then just inject that into you bottle and you have new air.

BTW blood,
how are your apple juices doing. did you ever see if they contaminated or did it worked out for you.


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OfflineChi Ro
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Re: Can I make more spores from spores? [Re: Digital Reality]
    #7456372 - 09/26/07 04:24 PM (16 years, 4 months ago)

6cc's of Spore Syringe Inoculant can easily be turned into 60 Syringes using agar's or BlutJager's Baby Bottle Teks.

It takes about a week for the baby bottles to fully colonize, so you'd be putting your project off for a week, but you'd have more than enough Liquid Culture Syringes to knock up 24 half-pint PF Style jars.


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Offlinevm711
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Registered: 09/16/07
Posts: 49
Last seen: 16 years, 1 month
Re: Can I make more spores from spores? [Re: Chi Ro]
    #7457422 - 09/26/07 08:19 PM (16 years, 4 months ago)

Thanks for all the responses.
I'm a patient guy, and certainly don't mind delaying the project to make a LC. My only concern is the pre sterilized jars I ordered from mushbox. How long will they be safe to use? I just got them in yesterday, can they wait another week before inoculation so i can make the liquid mycelium culture? The jars are all shrink wrapped if that makes a difference, though it probably doesnt...


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OfflineChi Ro
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Registered: 09/22/07
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Re: Can I make more spores from spores? [Re: vm711]
    #7457431 - 09/26/07 08:23 PM (16 years, 4 months ago)

They've already been sterilized so there is nothing inside the jars besides sterilized substrate. They'll be fine waiting the week it takes for you to make your Liquid Culture.


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Offlinevm711
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Last seen: 16 years, 1 month
Re: Can I make more spores from spores? [Re: Chi Ro]
    #7459041 - 09/27/07 07:16 AM (16 years, 4 months ago)

Thanks. Not that I doubt your word, but can anyone else confirm this? It's just that I once heard that they're only good after being sterilized for a week or so.


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Offlineveda_sticks
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Re: Can I make more spores from spores? [Re: vm711]
    #7459058 - 09/27/07 07:24 AM (16 years, 4 months ago)

the general census is that its better to inoc your substrate as soon as it is ready (once it has cooled enough) to give myc the best chance of getting hold of the substrate, the longer the jars sit, the more chance of a contaminate invading.

If u need to wait a week, as long as your jars have been pressure cooked correctly and the inoc holes are covered then it should be fine.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


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Invisiblejeetered
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Registered: 07/07/06
Posts: 3,055
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Re: Can I make more spores from spores? [Re: veda_sticks]
    #7459186 - 09/27/07 08:14 AM (16 years, 4 months ago)

It takes less then half of a cc to inoculate a full quart of Liquid Culture, you could concievably make about 15 quarts of LC from ONE syringe. Even multispore LC's are very usable, and much faster to colonize then spores alone.

When I FIRST started growing, I bought 5 of the same strain (syringes), that was damn near 100 bux, I could have bought ONE syringe, and made gallons of LC, which is what i do now, maybe u can skip learning the hard way, and listen to everyone here, make an LC, a quart jar LC.


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Offlinevm711
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Posts: 49
Last seen: 16 years, 1 month
Re: Can I make more spores from spores? [Re: jeetered]
    #7459313 - 09/27/07 08:48 AM (16 years, 4 months ago)

I would make a LC but I don't have a pressure cooker, is there another way to sterilize it? So basically I use water, hydrogen peroxide and honey in a jar, a couple ml of spore solution, drill a hole for access with an empty syringe and sterilize? Can I do this in a pan of boiling water for a little longer instead of using a PC?


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Invisiblesimplemachine
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Re: Can I make more spores from spores? [Re: vm711]
    #7459624 - 09/27/07 10:09 AM (16 years, 4 months ago)

You got it. Just make sure you use the right amount of media for the amount of water you're using. Skip the peroxide, and sterilize in boiling water, like so:


This is from Creamcorn's LC cloning tek

Quote:

Steam sterilization:
Place a cloth at the bottom of a pot with a tight fitting lid. Use an inch or two of water. Place your jar and syringe inside. Bring to a slow rolling boil. Once a boil has been achieved, start your timer for 30 minutes. At the end of 30 minutes, turn off the heat, walk away and don't even think about it for a few hours. Let it cool.




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Offlinevm711
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Re: Can I make more spores from spores? [Re: simplemachine]
    #7463283 - 09/28/07 06:58 AM (16 years, 4 months ago)

Great! Can I follow this tek and use the spores to inoculate the LC jar? I only have spores in a syringe, not mushrooms to clone.


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OfflineToo Vanilla
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Re: Can I make more spores from spores? [Re: vm711]
    #7463316 - 09/28/07 07:24 AM (16 years, 4 months ago)

ONe of the techs mentioned allows you to do just that.


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