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North Spore Shop: Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

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OfflineCyber
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Agar to grain question
    #7448530 - 09/24/07 05:57 PM (16 years, 4 months ago)

Ok, What is the best way to go from agar to grain?

I recently did a agar into a bag and had a couple contaminate. So Is it best to do agar to LC, or agar to pint jar then colonized pint jar to grain bag or maybe there is a better way that I am not aware of?

Thanks


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OfflineRogerRabbitM
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Re: Agar to grain question [Re: Cyber]
    #7448540 - 09/24/07 06:00 PM (16 years, 4 months ago)

You simply lift the lid of the grain jar and drop the agar wedge in. Prior to that, wipe the surfaces of the petri dish and grain jar with alcohol, flame sterilize the scalpel, wear surgical gloves that have been washed with alcohol, and work as fast as possible, leaving the agar culture exposed for only a few seconds, and the grain jar lid open no more than 2 seconds. It may be stating the obvious, but the use of a glovebox or flowhood should be considered mandatory.
RR


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OfflineCyber
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Re: Agar to grain question [Re: RogerRabbit]
    #7448560 - 09/24/07 06:05 PM (16 years, 4 months ago)

I am using a flow hood, I just sliced a 60mm dish of colonized agar in half and put half in each bag. So that would be about 5lb of grain to half of a 60mm dish.

The question was if it would be better to use an LC method or to work with pint or quart jars first then do a grain to grain.


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OfflineRogerRabbitM
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Re: Agar to grain question [Re: Cyber]
    #7448595 - 09/24/07 06:13 PM (16 years, 4 months ago)

No, you're fine like that. Let the mycelium recover from the wedge into the grains for a few days before you shake.
RR


--------------------
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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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North Spore Shop: Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies


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