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TomJoad
Goddamn Red


Registered: 07/13/03
Posts: 205
Loc: Oregon
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Strain selection w/ agar
#7448146 - 09/24/07 03:41 PM (16 years, 4 months ago) |
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Hey all you agar culturors out there- I was a-wonderin'. Once you've got your spores germinated and your dikaryons grown out, I hear that it is the rhizomorphic strains that you want. Now, it is my understanding (from reading the mushroom cultivator), that one in 3 substrains on average will be a fruiting strain. It is also my understanding that rhizomorphic mycelia is more likely to be a fruiting strain. Now, in your experience, can you comfortably say that a strain isolated from a nice, rhizomorphic sector will always be a fruiting strain? Could you say that a tomentose sector will never bear fruit? Are there any other traits we can look for in strain selection? And while we're at it, what does it mean if we have one sector that is a very faint blue while all the others are white? Thank you, o shroomery genii.
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somebody041
bud tester


Registered: 06/04/07
Posts: 476
Loc: California
Last seen: 9 years, 9 months
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Re: Strain selection w/ agar [Re: TomJoad]
#7448248 - 09/24/07 04:21 PM (16 years, 4 months ago) |
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I, too, have been wondering the answers to these questions...
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Workman
1999 Spore War Veteran



Registered: 03/01/01
Posts: 3,598
Loc: Oregon, USA
Last seen: 3 hours, 42 minutes
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Re: Strain selection w/ agar [Re: TomJoad]
#7448259 - 09/24/07 04:26 PM (16 years, 4 months ago) |
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I don't usually select much after spore germination. I just make sure the culture is clean and drop agar wedges with many strains into jars of sterile grain. I then clone the first fruit that appears and its usually a pretty good isolate. This also gives you the opportunity to select for other traits like color and shape from a single tray that are impossible to predict from a petri dish.
For cubensis, rhizomorphic mycelium is best and it always has fruited for me.
-------------------- Research funded by the patrons of The Spore Works Exotic Spore Supply My Instagram Reinvesting 25% of Sales Towards Basic Research and Species Identification 
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TomJoad
Goddamn Red


Registered: 07/13/03
Posts: 205
Loc: Oregon
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Re: Strain selection w/ agar [Re: Workman]
#7451692 - 09/25/07 12:37 PM (16 years, 4 months ago) |
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thanks Workman. Do the cultures need light and fae to fruit, or will they just spontaneously do it when ready?
any other thoughts, anyone?
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Workman
1999 Spore War Veteran



Registered: 03/01/01
Posts: 3,598
Loc: Oregon, USA
Last seen: 3 hours, 42 minutes
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Re: Strain selection w/ agar [Re: TomJoad]
#7451702 - 09/25/07 12:40 PM (16 years, 4 months ago) |
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I don't usually intend to fruit in the petri dish itself but that does happen. It does require light and only minimal air exchange.
-------------------- Research funded by the patrons of The Spore Works Exotic Spore Supply My Instagram Reinvesting 25% of Sales Towards Basic Research and Species Identification 
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dysphoria
lost soul


Registered: 06/04/03
Posts: 1,651
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Re: Strain selection w/ agar [Re: Workman]
#7451730 - 09/25/07 12:51 PM (16 years, 4 months ago) |
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fruiting a petri dish (in other words, letting one sit until spontaneous fruiting) is a good way of selecting the best fruiting strain, though very laissez-faire way to go about it.
they'll fruit when they're ready and my experience agrees with workman, it requires very little light or fae (if any) to trigger pinning.
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TomJoad
Goddamn Red


Registered: 07/13/03
Posts: 205
Loc: Oregon
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Re: Strain selection w/ agar [Re: dysphoria]
#7451833 - 09/25/07 01:14 PM (16 years, 4 months ago) |
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Hmm... and I have been hearing that tomentose substrains have been known to fruit well, though this goes against my previous understanding... just wondering how set in stone these "rules" are about selection. If, out of say 10 healthy substrains from one print, only one is obviously rhizomorphic, is it worth it to try to isolate some others as well?
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