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InvisibleMrKite1
Cosmo

Registered: 03/02/04
Posts: 1,384
Loc: AK
Hormodendrum cladosporioides
    #7446930 - 09/24/07 08:46 AM (16 years, 4 months ago)

I know this thread is dangerously close to being a sourcing topic which would then fall under the "No Off Topic" rule in this forum but I hope the obscurity of this topic here will make it one of the possible exceptions mentioned in that same rule.

Anyhow, I promised a friend of mine I would ask about this here. He is working on a project which will require a living culture of Hormodendrum cladosporioides, NRRL 8132.
I have been looking for a source for this since but without luck. So my primary question would be if anyone is aware of any programs which sample either living cultures of fungi or viable spores.

I believe he has done more research into the second issue than I have but if anyone had some resources regarding established cultivation techniques in the lab for this particular fungi it would be immensely helpful. I have begun a cursory search into this but have not turned much concrete information up. Time allowing I'm going to pour through some databases later today at school.

His intent is detailed in the quote below gleaned from an article he sent me.
Quote:


A solution of 0.025 part of 8-chloro-10,11-dihydrodibenz[b,f][1,4]oxazepine in 0.79 part acetone is added and incubation continued for an additional 48 hours. The culture broth is extracted three times with portions of methylene chloride. The methylene chloride extracts are combined, dried over anhydrous sodium sulfate, and stripped to dryness in vacuo.

The residue is dissolved in ethyl acetate and chromatographed on silica gel using a 3:97 ethyl acetate-methylene chloride mixture as eluant. Recrystallization of the crude product affords 8-chlorodibenz[b,f][1,4]oxazepine melting at 73 - 73.5.degree. C.




This article detailed the preparation of 8-chlorodibenz[b,f][1,4]oxazepine as a precursor for an antipsychotic. This is not his goal so his purification will not need to be as exacting.

Anyhow, I know I'm coming to the advanced forum with more questions than anything else but it seemed like the most suitable home for this thread. Any help will be greatly appreciated.


--------------------
When the going gets weird, the weird turn pro.


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Invisiblefastfred
Old Hand
Male User Gallery

Folding@home Statistics
Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
Re: Hormodendrum cladosporioides [Re: MrKite1]
    #7448599 - 09/24/07 06:14 PM (16 years, 4 months ago)

Well you have the NRRL number. NRRL is the ARS (NRRL) Culture Collection. So just go to their website and order it...

ht(.gov links munged)tp://nrrl.ncaur.usda.gov/

That's the only way to get the NRRL 8132 strain. Otherwise you could contact researchers that have published on this strain, but they might question why you are asking them and not just ordering it from the collection.

You might also try ATTC, as they are one of the largest collections around.

Otherwise, just go out into the field and get a sample. You can also just culture it from almost any forest soil.

BTW what the hell are you trying to do with it anyways?


-FF


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InvisibleMrKite1
Cosmo

Registered: 03/02/04
Posts: 1,384
Loc: AK
Re: Hormodendrum cladosporioides [Re: fastfred]
    #7448951 - 09/24/07 07:44 PM (16 years, 4 months ago)

Excellent, that answers my question entirely. I've found everything else I need at this point, so thank you fastfred. :smile:

Anyhow, I'm not doing anything with the stuff, this is a friend who
lives in a remote area further west.
His intent is to synthesize dibenzoxazepine. It's not a particular hobby project I would want to set out on but he has the necessary facilities and safety measures to do so.

He is all for personal protection without bullets but doesn't seem to think over the counter lachrymatory agents are effective enough in all situations, like animal or crack-head encounters.
Dibenzoxazepine is significantly more effective than other agents of the sort. It assures incapacitation and can easily be formulated for loading into blank shells.
So that is his intent as I understand it. :shrug:


--------------------
When the going gets weird, the weird turn pro.


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Invisiblefastfred
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Folding@home Statistics
Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
Re: Hormodendrum cladosporioides [Re: MrKite1]
    #7449041 - 09/24/07 08:07 PM (16 years, 4 months ago)

Hmmm.... Interesting.

Well good luck to him. Making dangerous substances isn't my idea of fun, but each to their own.


-FF


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