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OfflineAberael
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Registered: 07/01/07
Posts: 26
Last seen: 15 years, 8 days
cloning question
    #7446898 - 09/24/07 08:33 AM (16 years, 4 months ago)

I've been looking through the agar section in the teks and searched the forum, and haven't found an answer I need.

What do I do after the cloning has been successful and is growing on the agar in the petri dish? do I scrape it into a jar of substrate or similar?


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Offlinestrengthener
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Registered: 02/28/06
Posts: 119
Last seen: 15 years, 23 days
Re: cloning question [Re: Aberael]
    #7446922 - 09/24/07 08:42 AM (16 years, 4 months ago)

I would do a grain LC and then honey LC.


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OfflineAberael
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Registered: 07/01/07
Posts: 26
Last seen: 15 years, 8 days
Re: cloning question [Re: strengthener]
    #7446929 - 09/24/07 08:45 AM (16 years, 4 months ago)

I am a true newbie at this, so please excuse, but what does LC stand for?


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Offlinemike3000
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Registered: 09/05/07
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Last seen: 3 years, 7 months
Re: cloning question [Re: Aberael]
    #7446936 - 09/24/07 08:48 AM (16 years, 4 months ago)

Liquid Culture I think


--------------------
If we are here to figure out the meaning of life,
then isn't the meaning of life to find out why we are here?
Causing our existence to be pointless!


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Offlinestrengthener
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Re: cloning question [Re: mike3000]
    #7446948 - 09/24/07 08:51 AM (16 years, 4 months ago)

Just transfer a piece of agar to rye jar. when its fully colonized do a grain LC

Grain LC ---> http://www.shroomery.org/forums/showflat.php?Cat=0&Board=30&Number=6817701&PHPSESSID=&fpart=


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Offlinemike3000
Petski
Male


Registered: 09/05/07
Posts: 106
Last seen: 3 years, 7 months
Re: cloning question [Re: mike3000]
    #7446949 - 09/24/07 08:51 AM (16 years, 4 months ago)

Yet I am also a Newb so it could stand for living culture. Sorry for the added confusion!

I am used to LC standing for Liquid chromatography because of the course at college I'm taking.


--------------------
If we are here to figure out the meaning of life,
then isn't the meaning of life to find out why we are here?
Causing our existence to be pointless!


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Offlinestrengthener
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Male


Registered: 02/28/06
Posts: 119
Last seen: 15 years, 23 days
Re: cloning question [Re: mike3000]
    #7446950 - 09/24/07 08:52 AM (16 years, 4 months ago)

its Liquid culture :smile:


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OfflineAberael
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Registered: 07/01/07
Posts: 26
Last seen: 15 years, 8 days
Re: cloning question [Re: strengthener]
    #7446959 - 09/24/07 08:56 AM (16 years, 4 months ago)

woo! my heroes!

anything else I should know?


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Offlinestrengthener
Stranger
Male


Registered: 02/28/06
Posts: 119
Last seen: 15 years, 23 days
Re: cloning question [Re: Aberael]
    #7446985 - 09/24/07 09:04 AM (16 years, 4 months ago)

try reading as much as posible


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