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Offlinefirefairy323
Love is the law,love under will.
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Registered: 06/27/06
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Last seen: 8 years, 9 days
my lc wont form
    #7444007 - 09/23/07 12:48 PM (16 years, 4 months ago)

i inoculated 11 brf substrate jars with with one syringe on one day and then used the remaining 1 1/2 cc to inoculate a jar of honey water maybe 7 hours later the same day. all the brf jars are colonizing and some are pretty much finished. but the lc isn't doing much. and it's been almost three weeks. there is a tiny bit of white stringy stuff at the bottom of the lc and a few specks floating around. i did however leave the lc at room temp (which can get below 50*F in my house) a few days before placing it in the incubator. but now the lc has been in the incubator for 2 weeks. any advice?


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The tall flowers in my dreams are
  Big as the First State Bank,
    & they eat all the people
Except the ones I love.
                 
-Yusef Komunyakaa


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OfflineSkeeblix
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Registered: 01/28/07
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Re: my lc wont form [Re: firefairy323]
    #7444086 - 09/23/07 01:18 PM (16 years, 4 months ago)

LCs are really a hit and miss kind of thing. there's no real reliable method to tell if it's contaminated or not unless you shoot something up and see what grows off of it. If done right, they cut colonization times drastically, but personally, I hear more about failed LCs than anything else on this site.

Just out of curiosity, you did pasteurize the honey water before you went and shot it up, right?


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This post approved by:


Premedman1 said:
:lol: I just shat my pants.


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Offlinefirefairy323
Love is the law,love under will.
Female User Gallery


Registered: 06/27/06
Posts: 87
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Last seen: 8 years, 9 days
Re: my lc wont form [Re: Skeeblix]
    #7444113 - 09/23/07 01:26 PM (16 years, 4 months ago)

pasturize? i boiled the honey water in its jar for 30 mins and then let it cool for 7 hours before inoc. this is the tek i followed... http://www.shroomery.org/forums/showflat.php/Cat/0/Number/5238137/page/0/fpart/1/vc/1


--------------------
The tall flowers in my dreams are
  Big as the First State Bank,
    & they eat all the people
Except the ones I love.
                 
-Yusef Komunyakaa


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OfflineChi Ro
Jive Ass Turkey
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Registered: 09/22/07
Posts: 211
Loc: Right hurr!
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Re: my lc wont form [Re: firefairy323]
    #7444172 - 09/23/07 01:43 PM (16 years, 4 months ago)

How much honey did you add to your jar? Lack of honey could have something to do with it.


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Offlinefirefairy323
Love is the law,love under will.
Female User Gallery


Registered: 06/27/06
Posts: 87
Loc: everywhere
Last seen: 8 years, 9 days
Re: my lc wont form [Re: Chi Ro]
    #7444811 - 09/23/07 05:11 PM (16 years, 4 months ago)

i was immediately worried i put too much...2 fat teaspoons.


--------------------
The tall flowers in my dreams are
  Big as the First State Bank,
    & they eat all the people
Except the ones I love.
                 
-Yusef Komunyakaa


Extras: Filter Print Post Top
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