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Offlinehighc
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splicing spores
    #7443474 - 09/23/07 09:45 AM (16 years, 4 months ago)

what steps would need to be followed to combine two spores into one and grow them for qualities...such i want to mix brazilian and pink buffalo.


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OfflineAndrew47
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Re: splicing spores [Re: highc]
    #7443735 - 09/23/07 10:59 AM (16 years, 4 months ago)

Well, your end product is possible but your means aren't.

There's a very large topic on this where Workman had some great success mating monokaryotic cultures of two different cubensis strains in this very forum, search for it


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Offlinehighc
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Re: splicing spores [Re: Andrew47]
    #7443801 - 09/23/07 11:22 AM (16 years, 4 months ago)

indeed i will...thank you


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OfflineWorkmanV
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Re: splicing spores [Re: highc]
    #7444055 - 09/23/07 01:09 PM (16 years, 4 months ago)

Maybe I should contract out for this sort of thing. 

Workman's Cubensis Hybridization Service. 

Send two prints of different strains and get back around 20 F1 hybridized prints.  It would be up to the customer to work with the F1 spores to select out the desired traits through several generations.

Too bad I don't have the time to do this :wink:

What traits are you looking to combine?


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Offlinehighc
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Re: splicing spores [Re: Workman]
    #7444089 - 09/23/07 01:19 PM (16 years, 4 months ago)

the quick mycelium growth from brazilian potentcy from pink buffalo. looks of pink buffalo


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Invisibledysphoria
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Re: splicing spores [Re: highc]
    #7444531 - 09/23/07 03:46 PM (16 years, 4 months ago)

kind of a mix of phenotypes and genotypes you're wanting to select from there..
would be a bitch to isolate and separate them out

more along a long term project than any casual "hey lets make a hybrid"


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OfflineWorkmanV
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Re: splicing spores [Re: highc]
    #7444581 - 09/23/07 03:57 PM (16 years, 4 months ago)

Its hard to select potency without some way to do quantitative measurements of psilocybin and psilocin. But a faster growing pink buffalo strain seems reasonably simple to select for.


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OfflineCyber
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Re: splicing spores [Re: Workman]
    #7445220 - 09/23/07 06:50 PM (16 years, 4 months ago)

Quote:

Workman said:
Its hard to select potency without some way to do quantitative measurements of psilocybin and psilocin.




As soon as I get a GCMS Ill me in touch and we can make the most potent mushrooms out there. Then we can TAKE OVER THE WORLD! :lol:


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Offlinedanzick
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Re: splicing spores [Re: Workman]
    #7445295 - 09/23/07 07:12 PM (16 years, 4 months ago)

Quote:

Workman said:
Its hard to select potency without some way to do quantitative measurements of psilocybin and psilocin.





No problem, you just need a specific indicator in the agar that produces a quantitative colorometric change in the presence of the desired compound(s).  Piece of cake. :biggrin:


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OfflineWorkmanV
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Re: splicing spores [Re: danzick]
    #7445808 - 09/23/07 09:30 PM (16 years, 4 months ago)

Even if there was such an indicator, it wouldn't tell you much about the potency of the actual mushrooms, just of the mycelium on the agar. And even then, only if the substances were outside of the cells and not bound up within the mycelium.


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InvisiblePsychoslut
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Re: splicing spores [Re: Workman]
    #7446713 - 09/24/07 06:23 AM (16 years, 4 months ago)

these kids need to quit worrying about how potent the individual mushrooms are.
it is allot easier to grow a pound of dry mushrooms and extract psilocybin from them than it is to do all kinds of fancy agar crap and isolate potency and all that garbage.

JUST GROW MORE SHROOMS, ITS AS SIMPLE AS THAT.


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


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Offlinehighc
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Re: splicing spores [Re: Psychoslut]
    #7446757 - 09/24/07 07:16 AM (16 years, 4 months ago)

cyber please do get back to me on that ..........and psychoslut its not so much about the ease of growing lots of shroomz its more upon the level of difficult and the appreciation one would get out of delveloping a new strain of shroom that combines two seperate strains and maybe even having it mass produced. tell me you wouldnt buy a syringe of spores that produced up to 15 dry grams on a single cake with enough potentcy to eat one gram and hit a level four with the most visual experience one could have.....goin even further if we could get as precise to develop a way in which we could even change the color of a mushroom to our own specifications. not saying its gonna happen in a couple days but what if..............


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InvisiblePsychoslut
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Re: splicing spores [Re: highc]
    #7446795 - 09/24/07 07:49 AM (16 years, 4 months ago)

nothin but a pipe dream son, just give it up, unless you can get bill gates or warren buffet to sponsor your project you aint goin nowhere with it.


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


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Offlinehighc
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Re: splicing spores [Re: Psychoslut]
    #7446857 - 09/24/07 08:13 AM (16 years, 4 months ago)

bill gates???????? what would i need money for first things first you must completely understand the complex makeup of the properties in which i would be alternating to come up with final product so step 1 no money as for further down the road tasks i will worry bout that when the time comes...plus i make $750-$1500 a day so whatever money will be no problem.


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Offlinedanzick
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Re: splicing spores [Re: Workman]
    #7447364 - 09/24/07 11:26 AM (16 years, 4 months ago)

Quote:

Workman said:
Even if there was such an indicator, it wouldn't tell you much about the potency of the actual mushrooms, just of the mycelium on the agar.  And even then, only if the substances were outside of the cells and not bound up within the mycelium.




I know, Workman, and you're absolutely right.  I was just having some fun.  :biggrin:

Besides, as others have pointed out, obsession with potency doesn't make a lot of sense.


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Offlinehighc
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Re: splicing spores [Re: danzick]
    #7447424 - 09/24/07 11:44 AM (16 years, 4 months ago)

its not only potentcy i could just plant Psilocybe A. if i wanted stronger im really focusing in more of generating a completley new strain


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OfflineWorkmanV
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Re: splicing spores [Re: highc]
    #7447439 - 09/24/07 11:49 AM (16 years, 4 months ago)

If time is not an issue I would consider doing the initial cross if provided with the selected germplasm.


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Offlinehighc
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Re: splicing spores [Re: Workman]
    #7447742 - 09/24/07 01:39 PM (16 years, 4 months ago)

i have all the time in the world im actually going to create a slideshow on Power Point to provide other serious growers a tek. tis this will be a tedious task i shall set out and return with all but failure if given the right circumstances.


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Invisiblefastfred
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Re: splicing spores [Re: danzick]
    #7448576 - 09/24/07 06:08 PM (16 years, 4 months ago)

Quote:

danzick said:
No problem, you just need a specific indicator in the agar that produces a quantitative colorometric change in the presence of the desired compound(s).




pDMAB would probably be your best bet. Otherwise I've compiled a list of indicators that I thought might have a good chance of working.

You could also use a bunch of small scale extractions then TLC and visualize them with one of these reagents. You'd get a semi-quantitative result, but that should be plenty fine for your needs.

Metol (photographic chemical, Kodak's Elon)
Keller's reagent (glacial acetic acid, ferrous chloride, and concentrated sulfuric acid)
Ehrlich's reagent (4-dimethyl-aminobenzaldehyde in 50 ml of 90% ethanol and 50 ml of 38% hydrochloric acid)
Marquiss Test [1 volume formaldehyde solution (formalin) with 9 volumes of sulphuric acid]
pDMAB (p-dimethylaminobenzaldehyde)
Van Urk’s reagent (10% p-dimethylaminobenzaldehyde in conc. HCl)
para-dimethylaminocinnamaldehyde
potassium permanganate
pine tree shavings in HCl
One drop of 37% formaldehyde to 15 drops of concentrated sulfuric acid.
p-toluenesulfonic acid in methanol


-FF


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OfflineSheep
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Re: splicing spores [Re: highc]
    #7454633 - 09/26/07 07:01 AM (16 years, 4 months ago)

see the 40 pound shroom? take its size and the pink buffs potency.. One of them things could make the whole of Africa trip!


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