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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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anarchOi
Ellenalien is fat.



Registered: 08/06/07
Posts: 2,293
Loc: ASE
Last seen: 12 years, 11 months
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Best way to clone?
#7439882 - 09/22/07 09:41 AM (16 years, 4 months ago) |
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So i've been doing my best to go through old shroomery posts about cloning, and i've almost decided on the cardboard tek, but i saw alot of other interesting ways to clone, and none of them sound much alike
i'm curious as to what methods you all are using feel free to share any hybrid teks you use
i just want to get a strong cloned culture going so that i can take that step up to bulk
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thenewguy05
The Mushroom Man



Registered: 02/11/05
Posts: 2,123
Loc: My Underground Layer
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Re: Best way to clone? [Re: anarchOi]
#7439903 - 09/22/07 09:51 AM (16 years, 4 months ago) |
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in a glovebox: use a clean razor blade and slice the stem or cap in half and cu out a triangle wedge ad drop it into a Liquid culture jar (honey - 4 Grams & water - 96 Grams)
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xhooliganx
Munky


Registered: 03/09/06
Posts: 900
Loc: reno, nevada
Last seen: 2 years, 8 months
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ok first you're never supposed to slice something open to clone because you can push contaminates into the clean tissue from the surface. you tear it open grab the tip of the step and make it split in the middle and pull apart then cut a chunk out of the middle and put it into the lc.
but to answer you question the best way to make a clone is to take tissue from inside a stem and transfer it to agar. grow it out and make any transfers necessary to ensure a clean culture. then transfer to a slant for you master culture library, a half full jar of grain to use for making grain lc and a few jars to use as masters for g2g. that would be the optimal route if you really want to step up your game.
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anarchOi
Ellenalien is fat.



Registered: 08/06/07
Posts: 2,293
Loc: ASE
Last seen: 12 years, 11 months
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Re: Best way to clone? [Re: xhooliganx]
#7440294 - 09/22/07 12:01 PM (16 years, 4 months ago) |
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Does no one use the carboard tek?
would it be alright to use the agar substitute tek (BRF) with the live tissue clone? supposing i used a glove box and sterile tools
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xhooliganx
Munky


Registered: 03/09/06
Posts: 900
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Last seen: 2 years, 8 months
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Re: Best way to clone? [Re: anarchOi]
#7442425 - 09/23/07 12:49 AM (16 years, 4 months ago) |
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I don't think I have ever seen a single person use cardboard other that in that tek. you have a link to that agar substitute tek? try a tissue to grain transfer and let it grow out then make grain lc.
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ohmatic
searcher



Registered: 02/28/04
Posts: 6,742
Loc: europe
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Re: Best way to clone? [Re: xhooliganx]
#7442652 - 09/23/07 02:30 AM (16 years, 4 months ago) |
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simply usea agar. i use agar all the time, its perfect for these purposes.
mex-a agar plates:
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MONOTUB tek HEATBOMB tek RIP #cultivation! ....can't associate? well FUCK U !
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anarchOi
Ellenalien is fat.



Registered: 08/06/07
Posts: 2,293
Loc: ASE
Last seen: 12 years, 11 months
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Re: Best way to clone? [Re: ohmatic]
#7443182 - 09/23/07 08:15 AM (16 years, 4 months ago) |
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http://www.shroomery.org/8514/Agar-substitute
is it better to cut one big slice? or is it better to cut a bunch and break it up for multi-innoc points?
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xhooliganx
Munky


Registered: 03/09/06
Posts: 900
Loc: reno, nevada
Last seen: 2 years, 8 months
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Re: Best way to clone? [Re: anarchOi]
#7443775 - 09/23/07 11:11 AM (16 years, 4 months ago) |
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yeah that tek is weak. you can't do any kind of transfers for isolation. if you aren't going to use agar just transfer tissue to grain or lc. the other methods that you're looking at are pretty outdated.
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anarchOi
Ellenalien is fat.



Registered: 08/06/07
Posts: 2,293
Loc: ASE
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Re: Best way to clone? [Re: xhooliganx]
#7445931 - 09/23/07 10:02 PM (16 years, 4 months ago) |
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well hooligan, how bout if i did these in a special made mason jar that's only about an inch and a half tall then i could do isolation eh?
if i were to do it in a larger jar, i could just cut up the mycelia in the bottom, add some sterilized substrate and shake it up
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Cryogenicz
what?


Registered: 07/01/04
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Re: Best way to clone? [Re: ohmatic]
#7446153 - 09/23/07 11:06 PM (16 years, 4 months ago) |
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Ohm,
Those are pretty big wedges don't you think?
-Graham
-------------------- www.MycoPath.com Mushroom Spawn, Cultures, Fungi Bags, Casings, Master Grain Jars, Bags for In-vitro, Laboratory supplies, and much more! Mushroom Supplies. Fast Turnaround Times. Great Service. orders@mycopath.com enter code shroomery for 10% off product. www.FungiForum.com
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The shroomy 1
Luminous beings surround me




Registered: 03/27/07
Posts: 5,543
Loc: The Aether
Last seen: 5 months, 5 days
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Re: Best way to clone? [Re: Cryogenicz]
#7446390 - 09/24/07 12:56 AM (16 years, 4 months ago) |
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Quote:
Cryogenicz said: Ohm,
Those are pretty big wedges don't you think?
-Graham
LOL! I thought the same thing a first, but I've come to realize that size is relative! (take it from there!). LOL!
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AMU Q&A thread.
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jeanpignon
frenshchampignon

Registered: 08/07/07
Posts: 115
Loc: my mind,
Last seen: 5 years, 10 months
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i do this: -i take a nice shroom -i take a sample of clean tissue and dip it into 10% of 3% peroxide solution. -with the oven rack tek i transfer it direct on sterilized grain like g2g. -it work perfect to me because i have a perfect clone and i save time with the agar step -after once colonized you can use the spawn to make more spawn. -this work perfect for panaeolus because they colonize very fast. i hope this clear enough.
-------------------- bonjour les shroom!!!
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jeanpignon
frenshchampignon

Registered: 08/07/07
Posts: 115
Loc: my mind,
Last seen: 5 years, 10 months
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Re: Best way to clone? [Re: jeanpignon]
#7446509 - 09/24/07 02:28 AM (16 years, 4 months ago) |
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i was using the cardboard tek a lot whit great succes. but the question was: -why not direct on grain??? -can this work at all??
-------------------- bonjour les shroom!!!
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wutang
fungi



Registered: 06/28/07
Posts: 1,903
Last seen: 16 years, 2 months
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Re: Best way to clone? [Re: jeanpignon]
#7446677 - 09/24/07 05:50 AM (16 years, 4 months ago) |
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the agar substitute looks legit and great since i cant find that shit anywhere
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ohmatic
searcher



Registered: 02/28/04
Posts: 6,742
Loc: europe
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Re: Best way to clone? [Re: Cryogenicz]
#7446746 - 09/24/07 06:59 AM (16 years, 4 months ago) |
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Quote:
Cryogenicz said: Ohm,
Those are pretty big wedges don't you think?
-Graham
yes, however that's the way i like to do it
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MONOTUB tek HEATBOMB tek RIP #cultivation! ....can't associate? well FUCK U !
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Androctonus
Stranger



Registered: 09/17/05
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Loc: L.I.S.
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Re: Best way to clone? [Re: anarchOi]
#7446786 - 09/24/07 07:42 AM (16 years, 4 months ago) |
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-------------------- The red grass, up to my knees An aura comes towards me Someone's changed the formula Un-chaining another law The structures, the colors Uniform chaos is alternating The nature of things
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anarchOi
Ellenalien is fat.



Registered: 08/06/07
Posts: 2,293
Loc: ASE
Last seen: 12 years, 11 months
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well ive decided to make 2 clone cultures, one LC and one BRF Agar
modification to the honey LC being a tyvek filter and modification to the BRF Agar will be using the biopsy method stated in the honey LC tek
going to clone that car-crashed mushrom which is now like 7 inches and looking uber healthy, veil just broke 20 mins ago and my LC and Agar are PC'n as i type this
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jeanpignon
frenshchampignon

Registered: 08/07/07
Posts: 115
Loc: my mind,
Last seen: 5 years, 10 months
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Re: Best way to clone? [Re: anarchOi]
#7447450 - 09/24/07 11:52 AM (16 years, 4 months ago) |
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good boy!! well done jimy!
-------------------- bonjour les shroom!!!
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xhooliganx
Munky


Registered: 03/09/06
Posts: 900
Loc: reno, nevada
Last seen: 2 years, 8 months
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Re: Best way to clone? [Re: jeanpignon]
#7450993 - 09/25/07 09:17 AM (16 years, 4 months ago) |
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wutang I still have no clue why you can't find something as redily available as agar, but you can buy it super cheap online. you an get 2 lbs of the stuff for about 40$s shipped. that's enough to last 10 shroomerites a lifetime.
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xhooliganx
Munky


Registered: 03/09/06
Posts: 900
Loc: reno, nevada
Last seen: 2 years, 8 months
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Re: Best way to clone? [Re: jeanpignon]
#7451042 - 09/25/07 09:34 AM (16 years, 4 months ago) |
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wutang I still have no clue why you can't find something as redily available as agar, but you can buy it super cheap online. you an get 2 lbs of the stuff for about 40$s shipped. that's enough to last 10 shroomerites a lifetime.
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xhooliganx
Munky


Registered: 03/09/06
Posts: 900
Loc: reno, nevada
Last seen: 2 years, 8 months
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Re: Best way to clone? [Re: anarchOi]
#7451105 - 09/25/07 09:47 AM (16 years, 4 months ago) |
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Quote:
anarchOi said: well hooligan, how bout if i did these in a special made mason jar that's only about an inch and a half tall then i could do isolation eh?
if i were to do it in a larger jar, i could just cut up the mycelia in the bottom, add some sterilized substrate and shake it up
ok the thing about agar is that all of the growth happens on the surface. thus you get a perfectly flat plane of growth that allows you to remove selected sections that you want (rhizo sections, uncontaminated sections, sectors in general for isolation.) to transfer to a new dish.
now with brf you don't really have that option. you can only see the surface and you can have contaminates that are below the surface. its close to impossible to do a strain isolation from multispore. since you're cloning and don't have to worry about strain isolation basicly all you will be doing with the brf is colonizing it to see if it's contamed and transfering it another jar. so if you're doing that you would get better results transfering clone tissue dirrectly to grain, lc would be second choice to me the reason being is you won't know if you lc is dirty untill it colonizes and then you inject jars. the grain you'll know is clean if it colonized and has no contams it's just more time efficient and grain lc wil last a lot longer than sugar based lc.
oh and dontever trust the oven tek. it's total bullshit look up agars opinions on it and there's pleanty of science explaining why its actualy less clean than open air inoculation.
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Edited by xhooliganx (09/25/07 09:52 AM)
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drippingcandywet
Stranger



Registered: 04/28/14
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Quote:
jeanpignon said: i do this: -i take a nice shroom -i take a sample of clean tissue and dip it into 10% of 3% peroxide solution. -with the oven rack tek i transfer it direct on sterilized grain like g2g. -it work perfect to me because i have a perfect clone and i save time with the agar step -after once colonized you can use the spawn to make more spawn. -this work perfect for panaeolus because they colonize very fast. i hope this clear enough.
damn right
-------------------- just dripping around
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
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This thread is 6 years old, dipping the tissue in peroxide is fail, so is the oven tek.....
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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