Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Bulk Substrate, Injection Grain Bag   PhytoExtractum Kratom Powder for Sale   Original Sensible Seeds High THC Strains

Jump to first unread post Pages: < Back | 1 | 2  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinexhooliganx
Munky
Male User Gallery

Registered: 03/09/06
Posts: 900
Loc: reno, nevada
Last seen: 2 years, 8 months
Re: Best way to clone? [Re: anarchOi]
    #7451105 - 09/25/07 09:47 AM (16 years, 4 months ago)

Quote:

anarchOi said:
well hooligan,
how bout if i did these in a special made mason jar that's only about an inch and a half tall
then i could do isolation eh?

if i were to do it in a larger jar, i could just cut up the mycelia in the bottom, add some sterilized substrate and shake it up




ok the thing about agar is that all of the growth happens on the surface. thus you get a perfectly flat plane of growth that allows you to remove selected sections that you want (rhizo sections, uncontaminated sections, sectors in general for isolation.) to transfer to a new dish.

now with brf you don't really have that option. you can only see the surface and you can have contaminates that are below the surface. its close to impossible to do a strain isolation from multispore. since you're cloning and don't have to worry about strain isolation basicly all you will be doing with the brf is colonizing it to see if it's contamed and transfering it another jar. so if you're doing that you would get better results transfering clone tissue dirrectly to grain, lc would be second choice to me the reason being is you won't know if you lc is dirty untill it colonizes and then you inject jars. the grain you'll know is clean if it colonized and has no contams it's just more time efficient and grain lc wil last a lot longer than sugar based lc.

oh and dontever trust the oven tek. it's total bullshit look up agars opinions on it and there's pleanty of science explaining why its actualy less clean than open air inoculation.


--------------------


Edited by xhooliganx (09/25/07 09:52 AM)


Extras: Filter Print Post Top
Offlinedrippingcandywet
Stranger
Female User Gallery


Registered: 04/28/14
Posts: 12
Loc: ga
Last seen: 9 years, 5 months
Re: Best way to clone? [Re: jeanpignon]
    #20310462 - 07/22/14 10:35 AM (9 years, 6 months ago)

Quote:

jeanpignon said:
i do this:
-i take a nice shroom
-i take a sample of clean tissue and dip it into 10% of 3%
peroxide solution.
-with the oven rack tek i transfer it direct on sterilized grain
like g2g.
-it work perfect to me because i have a perfect clone and i save
time with the agar step
-after once colonized you can use the spawn to make more spawn.
-this work perfect for panaeolus because they colonize very fast.
i hope this clear enough.




damn right


--------------------
just dripping around


Extras: Filter Print Post Top
OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 4 hours
Trusted Cultivator
Re: Best way to clone? [Re: drippingcandywet]
    #20310471 - 07/22/14 10:40 AM (9 years, 6 months ago)

This thread is 6 years old, dipping the tissue in peroxide is fail, so is the oven tek.....


Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2  [ show all ]

Shop: North Spore Bulk Substrate, Injection Grain Bag   PhytoExtractum Kratom Powder for Sale   Original Sensible Seeds High THC Strains


Similar ThreadsPosterViewsRepliesLast post
* don't throw out your cardboard boxes and packing peanuts! TheLightIsOn 1,599 9 09/27/06 06:10 PM
by odium33
* multispore-clone-print/multispore-clone on and on like that etc.etc. question? Shnezbit 1,062 4 01/10/07 10:09 PM
by RogerRabbit
* Question on if I'm going about cloning the right way and if my expected outcome is right. ymhrswrider 689 7 11/27/04 04:55 AM
by MadHatR
* cloning an isolate to rejuvinate ?
( 1 2 all )
just me 2,333 21 07/21/09 03:38 PM
by just me
* Cloning wild fruits and beating contamination Stealthgrower 1,672 16 07/13/09 09:11 PM
by hyphae
* P. Subaeruginosa cardboard tek project sonicnature 10,209 10 11/26/10 03:58 PM
by UK Explorer
* help with cardboard grod31 1,179 8 01/17/08 08:31 PM
by grod31
* Cardboard cloning tek IGnosticAbhorI 6,873 11 11/03/07 01:59 AM
by This guy

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
46,482 topic views. 14 members, 120 guests and 33 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.021 seconds spending 0.01 seconds on 14 queries.