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Nature Boy
Stranger than most


Registered: 07/09/07
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What the heck is THIS (with photo)
#7435558 - 09/21/07 07:30 AM (16 years, 4 months ago) |
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On 9/17/07 (96 hours ago, exactly) I spore printed a massive PF shroom cap onto aluminum foil and the following day made up a (sterile) 10cc syringe utilizing the ENTIRE spore print. The syringe looked like an ink cartridge, it was so loaded with spores. I inoculated a set of six 1/2 pint BRF jars made in the standard way which had been PC'd the prior day at 15 psi for 120 minutes. When I inoculated the jars (with great attention to sterile technique) the purple spores were readily apparent as a thin blue-purple linear streak down the sides of the jars, but nothing like the wide area of charcoal color seen here. EVERY innoc point on each jar (24 separate points) look exactly the same as depicted.
As part of my standard post-inoculation technique, I examine each "remnant" in the syringe under 1000x using a Nikon Labophot, and take a microphotograph. Saw >>NO<< contaminants anywhere on the slide at 1000x, so it is hard to believe this represents contaminant, especially since there is a central focus of what apparently is expanding, healthy mycelium.
Is this simply "spread-out" spore stain? WHAT the heck is this...and should I allow it to continue, either as an accademic experiment to solve the riddle or immediately discard - without opening, obviously. Opinions? Thanks in advance. N.B.
-------------------- All submitted posts under this user name are works of pure fiction or outright lies. Any information, statement, or assertion contained therein should be considered pure unadulterated bullshit. Note well: Sorry, but I do not answer PM's unless you are a long-time trusted friend. If you have a question, ask it in the appropriate thread.
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dumbfounded1600
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Registered: 07/29/07
Posts: 2,624
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Re: What the heck is THIS (with photo) [Re: Nature Boy]
#7435850 - 09/21/07 09:37 AM (16 years, 4 months ago) |
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Get it..wear a respiratory mask because that BLACK Spores are highly Fatal if inhaled...Its worse then Ammonia and Bleach Mixed together...throw that jar and the substrate together as far away from your house as possible....
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Hotnuts
old hand


Registered: 02/26/05
Posts: 3,436
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They are not fatal. If that were the case, I would've been dead 3 times over.
Although spores from mold can cause rather harsh like flu symptoms in some cases, the chances of dying are almost none with adults. Infants are more prone to get very ill.
Edited by Hotnuts (09/21/07 01:21 PM)
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Nature Boy
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Posts: 8,241
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Re: What the heck is THIS (with photo) [Re: Hotnuts]
#7436760 - 09/21/07 02:03 PM (16 years, 4 months ago) |
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Hmmm...Stachybotrys is 10 x 20 microns, and would not have been missed on microscopy. Aspergillus and Penicillium are only ~ 1 micron, but E. Coli is an easy read at 2 microns, so this is a bit of a mystery.
Clearly, the entire syringe full of spores was contaminated. 24/24 inoculation sites and distribution immediately along the path of the inoculum can only mean the >>contents<< of the syringe contained the contaminant, if thats what this is...
Discarding the jars en toto is no hardship at all; they are presently WELL CONTAINED in a securely isolated environment. From now on I'll not be using any method for spore printing other than the essentially sterile one I developed (and have now refined a bit further) which has never contaminated one syringe. No more direct surface contact spore printing on aluminum foil for me.
Thanks to those who responded. Since all is secure, I'll let the jars mature one additional day just to see what ensues. 100% sure I'll discard, but I'm curious to see what develops in the interim. Might be instructive. N.B.
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Hotnuts
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Re: What the heck is THIS (with photo) [Re: Nature Boy]
#7436805 - 09/21/07 02:13 PM (16 years, 4 months ago) |
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Sheesh, I hope you didn't purchase that from a vendor?
Anyways. If you want to salvage the jars; simply pressure cook them all for 30 minutes prior to dumping out the contents. That will kill off the mycelium and spores associated with the mold.
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Nature Boy
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Registered: 07/09/07
Posts: 8,241
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Re: What the heck is THIS (with photo) [Re: Hotnuts]
#7437989 - 09/21/07 07:23 PM (16 years, 4 months ago) |
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Nah...no need to salvage the jars, they're cheap enough. They are headed for the incinerator (many miles away) tomorrow morning. N.B.
-------------------- All submitted posts under this user name are works of pure fiction or outright lies. Any information, statement, or assertion contained therein should be considered pure unadulterated bullshit. Note well: Sorry, but I do not answer PM's unless you are a long-time trusted friend. If you have a question, ask it in the appropriate thread.
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Androctonus
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Re: What the heck is THIS (with photo) [Re: Nature Boy]
#7438022 - 09/21/07 07:37 PM (16 years, 4 months ago) |
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"The syringe looked like an ink cartridge, it was so loaded with spores."
Sound familiar?
They are not contaminated, which should be obvious in the fact that you have mycelial growth within the staining.
That's right that's staining from that highly concentrated spore solution you used. You know your bugs and aseptic technique have some confidence in your skills.
-------------------- The red grass, up to my knees An aura comes towards me Someone's changed the formula Un-chaining another law The structures, the colors Uniform chaos is alternating The nature of things
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Nature Boy
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Re: What the heck is THIS (with photo) [Re: Androctonus]
#7439328 - 09/22/07 05:24 AM (16 years, 4 months ago) |
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Androctonus: Well, you might be right. I performed a re-review of the slides Here are a couple of representative images from the inoculum at 1000X. Only found >>ONE<< potential contaminant amongst hundreds of thousands of spores. The charcoal color has no associated mycelia, so how could it be spores? The bottom line is that there is no need to take a chance, and if I see this phenomena again with an utterly sterile inoculum, I'll then assume it is stain...but not today.


Regards, N.B.
-------------------- All submitted posts under this user name are works of pure fiction or outright lies. Any information, statement, or assertion contained therein should be considered pure unadulterated bullshit. Note well: Sorry, but I do not answer PM's unless you are a long-time trusted friend. If you have a question, ask it in the appropriate thread.
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CureCat
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Re: What the heck is THIS (with photo) [Re: Nature Boy]
#7442465 - 09/23/07 01:05 AM (16 years, 4 months ago) |
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If the black area grew larger or continues to cover surface area, it would certainly be mold. If not, then i suppose it could be spores, though it seems a bit far fetched.
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CaptainLinger
A Fungus Amongus


Registered: 05/25/07
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Re: What the heck is THIS (with photo) [Re: CureCat]
#7464312 - 09/28/07 01:12 PM (16 years, 4 months ago) |
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Spores seem absurdly far-fetched. That's *dark* black, with bright white mycelium in the middle. To simply assume it's not mold, because you had thick spores is bordering on the absurd; curecat is right.
Why did his "spores" form a distinct ring on the side of the jar, rather than moving with the water?
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