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Offlinedread
Power Ranger

Registered: 09/03/07
Posts: 229
Last seen: 9 years, 3 months
wait time
    #7434401 - 09/20/07 09:29 PM (16 years, 4 months ago)

So I know that the average time to see mycelium growth is normally 3-5 days sometimes up to a week, but it's been nearly two weeks and out of ten jars only one has about a quarter inch of mycelium. Should I just wait? Do you think the jars are still viable? Could there have been a problem with the spores?


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Offlinepazzy
Always Hardcore


Registered: 08/24/07
Posts: 1,360
Last seen: 13 years, 2 months
Re: wait time [Re: dread]
    #7434443 - 09/20/07 09:42 PM (16 years, 4 months ago)

2 weeks and not even a hint of mycellium? They might have a bacterial infection, or you could have inoculated while the jars were to hot killing the spores.


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Are you happy for a miracle?

:yinyang2:

Absolutely no source checks/discussions.


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InvisibleFeanor
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Registered: 05/07/06
Posts: 1,546
Re: wait time [Re: dread]
    #7434495 - 09/20/07 09:59 PM (16 years, 4 months ago)

Sometimes weird shit just happens!

How did you aquire the spores? Did you get them from a trustworthy company (shroomery sponsored)?

Two weeks is quite a long time. I don't really think there's any chance.

As has been said earlier, heat could have killed the spores if you inoculated the jars too early after pc-ing. If there's no sign of contamination, then, well, obviously, they aren't contaminated, so there just must be something wrong with the spores.


--------------------

May Terence McKenna Live Long

The DMT Chronicles


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Offlinedread
Power Ranger

Registered: 09/03/07
Posts: 229
Last seen: 9 years, 3 months
Re: wait time [Re: Feanor]
    #7434839 - 09/20/07 11:41 PM (16 years, 4 months ago)

I got the spores from Hawks Eye. The first batch I inoculated about 5 hours after sterilization and the second was well after that. Both times the jars and pot and water inside the pot were cold to the touch. There's no signs of contams. ugh..


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Offlinesoulsizzle
nobody f**kswith The Jesus


Registered: 05/17/05
Posts: 632
Last seen: 12 years, 5 months
Re: wait time [Re: dread]
    #7434957 - 09/21/07 12:23 AM (16 years, 4 months ago)

If there is growth in one or two but not very prolific growth, I would not blame the spores. I would check into ensuring you have the proper moisture content. What type of substrate/spawn are you using?

At this point, even if they all spontaneously busted into growth, they will have used a good portion of the substrates nutrients after all that time. I doubt they make it to 100% colonization before contamination sets in. However, there is no reason to throw them away just yet. It doesn't hurt to ride it out and hope for the best.


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OfflineMycoThrill
MycoFreak!
Male


Registered: 04/13/07
Posts: 243
Last seen: 13 years, 6 months
Re: wait time [Re: dread]
    #7434959 - 09/21/07 12:23 AM (16 years, 4 months ago)

5 hours sounds way too close to be cutting it. I suggest leaving it out overnight for 8-12 hours, but I'm not sure if after 5 hours the INSIDE of the cake would be cool enough. Remember, even if the outside is cool, the inside builds up eat (Earth's core effect?)


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"The world is a book, and those who do not travel read only one page." ---St. Augustine


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Offlinedread
Power Ranger

Registered: 09/03/07
Posts: 229
Last seen: 9 years, 3 months
Re: wait time [Re: MycoThrill]
    #7435051 - 09/21/07 12:53 AM (16 years, 4 months ago)

Thanks for all the replies guys. So how would i go about checking moisture content?


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Offlinetight
Stranger
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Registered: 02/26/07
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Re: wait time [Re: dread]
    #7435096 - 09/21/07 01:16 AM (16 years, 4 months ago)

Basically I think the goal is to have enough water in your medium so that it is saturated but there isn't water visible against the glass or pooling or anything like that. Don't quote me on it though!


--------------------
SD prop 215 and occasional fungus cultivator


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Offlinedread
Power Ranger


Registered: 09/03/07
Posts: 229
Last seen: 9 years, 3 months
Re: wait time [Re: tight]
    #7435176 - 09/21/07 02:49 AM (16 years, 4 months ago)

Hmm well I thought I had plenty of water but at this point I would imagine that some of it may be gone. Is it advisable to add more water at this point? Also has anyone else had a similar situation?


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Offlinemushroommarren
fungi fanatic
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Registered: 09/21/07
Posts: 49
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Re: wait time *DELETED* [Re: dread]
    #7435252 - 09/21/07 03:42 AM (16 years, 4 months ago)

Post deleted by mushroommarren

Reason for deletion: j



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Offlinejuris1
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Registered: 08/08/07
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Re: wait time [Re: mushroommarren]
    #7436157 - 09/21/07 11:18 AM (16 years, 4 months ago)

If you're only four days just give it some time. I am towards the end of my first grow and I also incubated my jars in my closet in a shoe box with a blanket/towel on top of them. My temps were 78-80F, and they all colonized fine. some took two weeks to fully colonize while some others took almost three weeks. You should be good!


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"In Vino Veritas"

Mycology (from the Greek μύκης, meaning "fungus") is the study of fungi, their genetic and biochemical properties, their taxonomy, and their use to humans as a source for tinder, medicinals (e.g., penicillin), food (e.g., beer, wine, cheese, edible mushrooms) and entheogens, as well as their dangers, such as poisoning or infection.



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Invisibledumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
Re: wait time [Re: juris1]
    #7436176 - 09/21/07 11:22 AM (16 years, 4 months ago)

I would just add like 10cc...2 cc of Distilled or if you have good tap water, too each jar...thats just me though

yeah 5 hours seem way too early...should have waited until the PC and jars have cooled


Edited by dumbfounded1600 (09/21/07 11:35 AM)


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Offlineskattman1982
Stranger
Male

Registered: 09/18/07
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Last seen: 7 years, 11 months
Re: wait time [Re: dumbfounded1600]
    #7436200 - 09/21/07 11:32 AM (16 years, 4 months ago)

definitely think 5 hours after pc was too close. you could try injecting a cc or so of sterilized water and loosening your lids a touch but after two weeks i might start another batch.


--------------------
They lie about marijuana. Tell you pot-smoking makes you unmotivated. Lie! When you're high, you can do everything you normally do, just as well. You just realize that it's not worth the fucking effort. There is a difference.-Bill Hicks-

by the way, everything i write is for entertainment purposes and is purely fictional. do not construe these written words with the author having any knowledge of actual persons, places, events, or actions.


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Offlinesoulsizzle
nobody f**kswith The Jesus


Registered: 05/17/05
Posts: 632
Last seen: 12 years, 5 months
Re: wait time [Re: skattman1982]
    #7438525 - 09/21/07 10:06 PM (16 years, 4 months ago)

You shouldn't really ever introduce water to jars after the fact. It's all about getting the moisture content right in the first place. What kind of substrate do you have in these jars?


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Offlinedread
Power Ranger


Registered: 09/03/07
Posts: 229
Last seen: 9 years, 3 months
Re: wait time [Re: soulsizzle]
    #7439161 - 09/22/07 02:20 AM (16 years, 4 months ago)

brf and verm


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Offlinedread
Power Ranger


Registered: 09/03/07
Posts: 229
Last seen: 9 years, 3 months
Re: wait time [Re: dread]
    #7442269 - 09/22/07 11:39 PM (16 years, 4 months ago)

well the the mycelium on the one jar has doubled in size.


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Offlinedread
Power Ranger


Registered: 09/03/07
Posts: 229
Last seen: 9 years, 3 months
Re: wait time [Re: dread]
    #7465222 - 09/28/07 06:32 PM (16 years, 4 months ago)

Another update: The first jar with mycelium is about halfway colonized now and another jar has started. So it has been a very rough start but I might yet get something out of this.


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