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OfflineHankHill
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Registered: 04/07/06
Posts: 73
Last seen: 16 years, 1 month
Please help with possible contaminations
    #7432904 - 09/20/07 03:21 PM (16 years, 4 months ago)



this is the LC jar, the yellow/(green?) is hard to see in the picture unfortunately,
but as i said it is not what most of the jar is, most of it is white


this is a new lc i made, there are small round clunky things in it ? what are these?


this is my only spore jar i have some doubts on, it just in general looks funky


then note what i have circled in red, there is this small
little circular thing that seems to have a slight
yellow / (green?) tinge but, not really, but, sort of .


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Offlineveda_sticks
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Re: Please help with possible contaminations [Re: HankHill]
    #7433115 - 09/20/07 04:07 PM (16 years, 4 months ago)

its really hard to tell with those pictures.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


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OfflineHankHill
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Registered: 04/07/06
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Last seen: 16 years, 1 month
Re: Please help with possible contaminations [Re: veda_sticks]
    #7433196 - 09/20/07 04:18 PM (16 years, 4 months ago)

i know :blush:

if the jar winds up so that the green is eclipsed by white, is it usable?

do contaminated jars get completely covered with mold or is it just in spots?

i suppose it definitely is green mold though hummm

what is with the chunky things in the liquid culture? this perplexes me.



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Invisibledumbfounded1600
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Registered: 07/29/07
Posts: 2,624
Re: Please help with possible contaminations [Re: HankHill]
    #7433226 - 09/20/07 04:23 PM (16 years, 4 months ago)

The LCS Contammed if its yellow and Green


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OfflineHankHill
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Registered: 04/07/06
Posts: 73
Last seen: 16 years, 1 month
Re: Please help with possible contaminations [Re: dumbfounded1600]
    #7433835 - 09/20/07 07:17 PM (16 years, 4 months ago)

am i guaranteed that the other 4 jars will contam ?

i will have to boil and recycle this one and destroy the lc,

i suppose eventually i ought to be able to make a good lc, and they only take a few days, but shooting jars takes much longer

HUM!:evil:


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OfflineHankHill
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Registered: 04/07/06
Posts: 73
Last seen: 16 years, 1 month
Re: Please help with possible contaminations [Re: HankHill]
    #7434217 - 09/20/07 08:46 PM (16 years, 4 months ago)

i'm trying to figure out why the LC's contam

when i sterilize the syringe for them i have simply put the syringe in a pot of water and steam cleaned it, but have never sucked in boiling water and spit it out to clean

is this faulty ? i just now read about the boiling water bit and that sounds much more intelligent, quick, and helpful for sterilizing a syringe than this.

when i make it i put the honey and water in a jar then boil them, then put it all in the glove box and shoot it up after flame sterilizing the syringe needle

it ought not be a problem

the tek i read says to boil the water inside the jar, THEN add honey, then boil them briefly,
why, is this necessary?


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OfflineMadox_01
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Re: Please help with possible contaminations [Re: HankHill]
    #7434339 - 09/20/07 09:09 PM (16 years, 4 months ago)

Wrap you syringes in Tinfoil then PC for 15 minutes at 15 PSI. Generally I PC my LC mixture at the same time as a few empty syringes - 15 min.

Also- what kind of filtration do you have on your LC jars?


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OfflineHankHill
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Registered: 04/07/06
Posts: 73
Last seen: 16 years, 1 month
Re: Please help with possible contaminations [Re: Madox_01]
    #7435895 - 09/21/07 09:56 AM (16 years, 4 months ago)

i put medical tape over the incoulation point and cover it with tinfoil

for some reason the seal is not airtight and shaking it has let liquid out

and i know this is most definitely not good.

i killed the bad jars, and one of the lcs that i did in a regular 1/2 pint jar formed an immensely strong seal somehow, perhaps from the extensive boiling

if you boil for a long amount of time does this cause the jars to seal heavily so that this is not an issue?

of my spore jars i have one that is fully colonized now and 2 that are very close

some of the even most heavily stalled looking ones have apparently made a lot of headway

this is all due to flipping

are you supposed to un-flip the jars once they fully colonize, or will they grow faster upside down for pinning?


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Offlineveda_sticks
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Re: Please help with possible contaminations [Re: HankHill]
    #7435920 - 09/21/07 10:06 AM (16 years, 4 months ago)

very odd, the jars should seal wihtout tightening the lid very tight, should only hand tighten it, when using micropore tape, tinfoil shouldnt be necesary, only when pressure cooking. there is much debate over weather flipping jars actually speeds up colinisation, its suggested if jars stall. theory being that co2 is heavy and builds up in the bottom of the jars, flip the jar and the co2 then falls to what was the top.

i flipped 1 of my jars and it seemed to start colinising again, other people have reported no change. try it and see.

i thin u may have gotten a little confused, once they are 100% colinised u give it some extra time to get a good hold of the substrate (extra 5-7 days) then they are birthed (recomend dunk and roll) to the fruiting chamber where u initiate pinning by introducing light humidity and temperature change.

If ur are are sterilised properly and ur inoc precedure good, then chances of contams are minimal, have not heard of boiling longer sealing the jars better, the jars should already have a good seal.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


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OfflineHankHill
Stranger
Registered: 04/07/06
Posts: 73
Last seen: 16 years, 1 month
Re: Please help with possible contaminations [Re: veda_sticks]
    #7436280 - 09/21/07 11:54 AM (16 years, 4 months ago)

in the videos they show that the mushrooms begin growing inside the jar and that is how you tell when to birth them

my procedures are good because the jars with normal spores are all fine as far as i can tell

so i just wait 5 days and then birth regardless of whether mushrooms start to grow in the jars?


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