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civicracerx7



Registered: 12/30/05
Posts: 397
Last seen: 7 years, 2 months
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lost them all to green mold
#7432877 - 09/20/07 03:16 PM (16 years, 4 months ago) |
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Im so bummed! I've had great results many times before
and this time I even used a GB (never even used one B4)
flamed in between jars and wiped with alcohol
just cant figure it out.
pressure cooked the jard for an hour at 15 psi the nite b4
any ideas?
Imma try it again in a week hopefulyl without the green mold
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CaptainLinger
A Fungus Amongus


Registered: 05/25/07
Posts: 1,756
Last seen: 3 years, 7 months
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Re: lost them all to green mold [Re: civicracerx7]
#7433006 - 09/20/07 03:41 PM (16 years, 4 months ago) |
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Hard to tell, considering you don't even mention what's in the jars.
If all of them contaminated, I'd suspect a lot of green mold in the environment. Use a glovebox.
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RoachMan
Old Man




Registered: 03/06/05
Posts: 2,083
Loc: Midwest
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Re: lost them all to green mold [Re: CaptainLinger]
#7433051 - 09/20/07 03:52 PM (16 years, 4 months ago) |
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Quote:
civicracerx7 said: ...and this time I even used a GB (never even used one B4)...
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highc
creator



Registered: 08/31/07
Posts: 3,592
Loc: maryland
Last seen: 7 years, 2 months
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Re: lost them all to green mold [Re: RoachMan]
#7433068 - 09/20/07 03:56 PM (16 years, 4 months ago) |
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it happens to the best of em just remember it only takes one microscopic bacteria to slip through to ruin a jar its ok though even the most experienced have a bad jar every once in a while
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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 25 days
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Re: lost them all to green mold [Re: highc]
#7433081 - 09/20/07 03:59 PM (16 years, 4 months ago) |
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i just lost 3 jars, 4 jars made up from a brand new syringe from a reliable source. and 4 days later - green mold growing from the middle of the substrate in 3 jars.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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civicracerx7



Registered: 12/30/05
Posts: 397
Last seen: 7 years, 2 months
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Re: lost them all to green mold [Re: veda_sticks]
#7436987 - 09/21/07 02:49 PM (16 years, 4 months ago) |
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I think ive figured it out
I believe there was some mold in the jars that wasnt washed out the correct way as this particular jar set had molded mycelium and was thrown out
I did wash the jars but I tihnk theyll need the be PC'ed or bleached
what needs to be done once jars have had mold in them to sterilize?
will bleach do the trick?
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orchidfanatic
retiree




Registered: 08/12/07
Posts: 832
Loc: where the wild things are
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Re: lost them all to green mold [Re: civicracerx7]
#7437011 - 09/21/07 02:56 PM (16 years, 4 months ago) |
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yeah bleach will do it ..how long did you PC the jars?
Edited by orchidfanatic (09/21/07 02:57 PM)
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soulsizzle
nobody f**kswith The Jesus


Registered: 05/17/05
Posts: 632
Last seen: 12 years, 5 months
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Re: lost them all to green mold [Re: orchidfanatic]
#7438551 - 09/21/07 10:12 PM (16 years, 4 months ago) |
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Soap and water works fine. Anything left over will be killed when you PC your grains.
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civicracerx7



Registered: 12/30/05
Posts: 397
Last seen: 7 years, 2 months
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Re: lost them all to green mold [Re: soulsizzle]
#7439744 - 09/22/07 08:39 AM (16 years, 4 months ago) |
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I PC'ed for an hour at 15psi I believe the incubator and everything has got spores all in it
Im gonna soap and water and bleach everything try and get everythign sterilized and try again
its just weird Ive had great success before
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CaptainLinger
A Fungus Amongus


Registered: 05/25/07
Posts: 1,756
Last seen: 3 years, 7 months
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Re: lost them all to green mold [Re: orchidfanatic]
#7439878 - 09/22/07 09:39 AM (16 years, 4 months ago) |
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You should've PC'd the jars *before* spreading mold spores all over their house. They then require no further sterilization. Rinse out, use again.
Bleach will not "do it". It will not kill live molds.
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civicracerx7



Registered: 12/30/05
Posts: 397
Last seen: 7 years, 2 months
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Re: lost them all to green mold [Re: CaptainLinger]
#7440728 - 09/22/07 02:45 PM (16 years, 4 months ago) |
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ok so what should I do?
PC the jars before putting the substrate in and after?
and what about the mold spores now floating around in abundance?
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veilbreaker
Sour girl


Registered: 06/12/07
Posts: 528
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Re: lost them all to green mold [Re: civicracerx7]
#7442012 - 09/22/07 10:13 PM (16 years, 4 months ago) |
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Quote:
civicracerx7 said: ok so what should I do?
PC the jars before putting the substrate in and after?
and what about the mold spores now floating around in abundance?
PC them before you open them.
-------------------- People seem not to see that their opinion of the world is also a confession of their character. ~Ralph Waldo Emerson
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