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highc
creator



Registered: 08/31/07
Posts: 3,592
Loc: maryland
Last seen: 7 years, 2 months
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possible inoculation idea
#7432263 - 09/20/07 01:11 PM (16 years, 4 months ago) |
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if you prepared lets say a rye jar then just took a freshly open cap or a piece of a cap and put it on top inside the closed jar for a couple hours then removed will it grow?????
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RoachMan
Old Man




Registered: 03/06/05
Posts: 2,083
Loc: Midwest
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Re: possible inoculation idea [Re: highc]
#7432273 - 09/20/07 01:14 PM (16 years, 4 months ago) |
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Quite possibly, however, you are introducing contaminants whenever you open the jar (unless you use a glovebox or flowhood, and is still not assurable).
Thus is why people do spore prints, LC, grain to grain, etc.
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highc
creator



Registered: 08/31/07
Posts: 3,592
Loc: maryland
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Re: possible inoculation idea [Re: RoachMan]
#7432330 - 09/20/07 01:25 PM (16 years, 4 months ago) |
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your not introducing contams anymore then with the holes in the jars if you take the same precautions it should be fine i will experiemnt when mine flourish.
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RoachMan
Old Man




Registered: 03/06/05
Posts: 2,083
Loc: Midwest
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Re: possible inoculation idea [Re: highc]
#7432341 - 09/20/07 01:27 PM (16 years, 4 months ago) |
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You are saying you're not introducing any more contaminants by opening the jar lid?
That is obsurd.
Removing the lid for even a moment in an open room will most likely lead to contamination.
Why even ask the question if you're going to refute the answer(s)?
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highc
creator



Registered: 08/31/07
Posts: 3,592
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Re: possible inoculation idea [Re: RoachMan]
#7432371 - 09/20/07 01:33 PM (16 years, 4 months ago) |
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well i mean there is more surface area exposed but like i said with oust and a glovebox combined with your vermiculite barrier i dont see a problem.....also my jars compose of 1 nickel or dime sized whole, which i fill with cotton for gas exchange so i could cut a lil piece and slip it in only thing im thinkin is i dont know what could be floatin around on the mushroom and if the mushroom starts to rot it could pose a problem. but i will give it a go
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orchidfanatic
retiree




Registered: 08/12/07
Posts: 832
Loc: where the wild things are
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Re: possible inoculation idea [Re: highc]
#7432391 - 09/20/07 01:38 PM (16 years, 4 months ago) |
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by far the best way to inoculate is by agar .. you see you can germinate spores on agar put LC on agar and put tissue on agar . all will show you if its contaminated .. just cut out the mycelium and transfer to another plate .. till you have a clean culture with no contams .. then cut the agar piece out and put into jars in a still air box glove box or flowhood..
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highc
creator



Registered: 08/31/07
Posts: 3,592
Loc: maryland
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yeah i know of the agar i wasnt really lookin for an easy way just maybe a new one.
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RoachMan
Old Man




Registered: 03/06/05
Posts: 2,083
Loc: Midwest
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Re: possible inoculation idea [Re: highc]
#7432534 - 09/20/07 02:09 PM (16 years, 4 months ago) |
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Quote:
highc said: well i mean there is more surface area exposed but like i said with oust and a glovebox combined with your vermiculite barrier i dont see a problem
You don't see a problem? I didn't realize you were talking about a PF cake. The spores won't make it through the verm barrier in order to germinate.  That is why we use a needle to poke through the verm layer.
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highc
creator



Registered: 08/31/07
Posts: 3,592
Loc: maryland
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Re: possible inoculation idea [Re: RoachMan]
#7432557 - 09/20/07 02:14 PM (16 years, 4 months ago) |
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no im going to use a rye jar...but yes you are correct thank you for that correction. but i wasnt thinkin cause i never use a barrier i just over do the sterile process. dont know y but havent had a problem yet with my own made jars.
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simplemachine
Manfly


Registered: 09/14/03
Posts: 1,981
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Re: possible inoculation idea [Re: highc]
#7432560 - 09/20/07 02:15 PM (16 years, 4 months ago) |
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"1 nickel or dime sized whole, which i fill with cotton for gas exchange"
Wow. Doesn't it take giant fist full of polyfill to fill that hole?
I would make the holes more like 1/4" in diameter. Any bigger is pointless for GE and would probably let your substrate dry out.
Also you mentioned the dry verm layer...If you plan on placing a cap on top of the dry verm, how would the spores penetrate that to get to the brf/verm substrate below?
Not trying to be a jerk, but as with most nooby tek ideas, the answer is, "If it worked, we'd already be doing it."
Its great to experiment, but I think your energy would be better spent in other areas.
Have fun!
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RoachMan
Old Man




Registered: 03/06/05
Posts: 2,083
Loc: Midwest
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Re: possible inoculation idea [Re: highc]
#7432580 - 09/20/07 02:19 PM (16 years, 4 months ago) |
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Plugging the holes with polyfill or using a tyvek disc would work for PF tek as well, and would also allow you to get a tish more substrate into each jar.
Seeing that (in my area) tyvek is cheaper than verm...this would be the way to go.
Quote:
simplemachine said: Its great to experiment, but I think your energy would be better spent in other areas.
Like making prints, making syringes from prints, and improving your sterile technique even more.  Everyone that isn't a "pro" could use some practice at all of this, AND even some of the PROS will admit that everyone can benefit from practice.
-------------------- "The mistake that I make is to try to come awake in a place you're just supposed to get shit faced or baked."
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highc
creator



Registered: 08/31/07
Posts: 3,592
Loc: maryland
Last seen: 7 years, 2 months
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Re: possible inoculation idea [Re: RoachMan]
#7432594 - 09/20/07 02:22 PM (16 years, 4 months ago) |
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yeah but im sayin rye jars and if it worked weed be doin it huh.. well where were cars in the early 1800's????? someone might find a way to fully colonize jars in 24 hours.... ya never know
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RoachMan
Old Man




Registered: 03/06/05
Posts: 2,083
Loc: Midwest
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Re: possible inoculation idea [Re: highc]
#7432612 - 09/20/07 02:26 PM (16 years, 4 months ago) |
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Quote:
highc said: someone might find a way to fully colonize jars in 24 hours.... ya never know
Only if they are going to change the entire genetics of the cubensis species will that occur, but yeah...I don't count anything out. 
The only benefit that I could see to your "tek" would be that the spores wouldn't need to rehydrate in vitro because they were already fresh and hydrated.
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highc
creator



Registered: 08/31/07
Posts: 3,592
Loc: maryland
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Re: possible inoculation idea [Re: RoachMan]
#7432749 - 09/20/07 02:56 PM (16 years, 4 months ago) |
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well ill give it a go and post all the pics its worth the $2 (aproximate cost of jar and ingredients)
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RoachMan
Old Man




Registered: 03/06/05
Posts: 2,083
Loc: Midwest
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Re: possible inoculation idea [Re: highc]
#7432766 - 09/20/07 02:57 PM (16 years, 4 months ago) |
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Go for it. 
Make sure to post back!
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highc
creator



Registered: 08/31/07
Posts: 3,592
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Re: possible inoculation idea [Re: RoachMan]
#7432794 - 09/20/07 03:02 PM (16 years, 4 months ago) |
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i will keep a look for..........Fresh shroom inoculation
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RoachMan
Old Man




Registered: 03/06/05
Posts: 2,083
Loc: Midwest
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Re: possible inoculation idea [Re: highc]
#7432835 - 09/20/07 03:09 PM (16 years, 4 months ago) |
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Well, "fresh mushroom inoculation" would imply inoculating the grain with fresh mushroom tissue. 
You could just reply back in this same thread.
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CaptainLinger
A Fungus Amongus


Registered: 05/25/07
Posts: 1,756
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Re: possible inoculation idea [Re: RoachMan]
#7432999 - 09/20/07 03:40 PM (16 years, 4 months ago) |
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You could just use a scalpel to inoculate the spawn with fresh mycelium.
I really don't see a point to using a multispore spawn. Why not just clone? You're opening the jar twice, introducing a huge chunk of nonsterile tissue...and for what?
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highc
creator



Registered: 08/31/07
Posts: 3,592
Loc: maryland
Last seen: 7 years, 2 months
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i could reply back but my jars right now are only like 10% colonized so i got a couple weeks before i see my shroomz
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Mycomyth
Demented Avenger



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Re: possible inoculation idea [Re: highc]
#7433112 - 09/20/07 04:06 PM (16 years, 4 months ago) |
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Quote:
highc said: someone might find a way to fully colonize jars in 24 hours.... ya never know
Not with spores from a piece of cap, you won't. I'd be willing to make a sizeable bet that if you open a jar (ESPECIALLY of rye), and place a dirty ass mushroom cap in there, what you grow in there will not be mushroom mycellium.
M
-------------------- Wave upon wave of demented avengers marched cheerfully out of obscurity into the dream.
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CaptainLinger
A Fungus Amongus


Registered: 05/25/07
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Re: possible inoculation idea [Re: highc]
#7433116 - 09/20/07 04:07 PM (16 years, 4 months ago) |
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That has nothing to do with the reason to do so, which remains unclear.
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highc
creator



Registered: 08/31/07
Posts: 3,592
Loc: maryland
Last seen: 7 years, 2 months
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yeah i didnt mean this would colonize jar in 24 hours just sayin someday someone might find a way
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CaptainLinger
A Fungus Amongus


Registered: 05/25/07
Posts: 1,756
Last seen: 3 years, 7 months
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Re: possible inoculation idea [Re: highc]
#7433156 - 09/20/07 04:12 PM (16 years, 4 months ago) |
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That still makes absolutely no sense. Your "idea" has no advantage over cloning a mushroom, which is an established technique.
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