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Shop: Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag

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Offlinemycmyc
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Registered: 09/07/07
Posts: 21
Last seen: 4 years, 4 months
Half Contam'd / Half No Germinate
    #7432239 - 09/20/07 01:03 PM (16 years, 4 months ago)

This is strange to me. I PC'd 10 jars of soaked, simmered and strained WBS for 1 hour at 15 psi. The first 5 were innoc'd with Golden Teacher syringe and set in incubation. The second batch of 5 was innoc'd with South American syringe and set in incubation. Within 3 days trich ripped through the GT jars and now at 9 days later there is no sign of germination or contams in the South American jars.
I have no clue how this occurs. Jars were innoc'd in a sterile glove box and I used tyvek filter, polyfill filter and aluminum foil as a cover. Also, the lids were wiped with an alcohol swap after innoculating. Jars were incubated at 82 degrees. Any ideas? I think I might stick to cakes for a while.


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OfflineCaptainLinger
A Fungus Amongus


Registered: 05/25/07
Posts: 1,756
Last seen: 3 years, 7 months
Re: Half Contam'd / Half No Germinate [Re: mycmyc]
    #7464286 - 09/28/07 01:05 PM (16 years, 4 months ago)

Has nothing to do with your sterilization, or the contents of your jars. Trich hit = added at inoculation. Molds die somewhere around 130F; you could boil for 30m and sufficiently eliminate them.

Your jar setup seems overly elaborate; a simple tyvek filter over the existing disk will do. The aluminum foil, if you do use it, should be removed immediately after you PC.


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OfflineRogerRabbitM
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Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: Half Contam'd / Half No Germinate [Re: CaptainLinger]
    #7465073 - 09/28/07 05:37 PM (16 years, 4 months ago)

Don't leave the foil on after the PC. Its only purpose is to prevent water that drips from the lid getting into the jars and ruining the moisture content. If you leave it in place, the filter stays damp and can easily allow contaminants to colonize right through it. It sounds like contaminated inoculant though in this case.
RR


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