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Invisiblejoshua m
Not a NoOb
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Registered: 07/14/07
Posts: 168
Loc: MiD wEsT
Inoculation of liquid culture
    #7431310 - 09/20/07 07:27 AM (16 years, 4 months ago)

OK so i have a half gallon jar of lc of Golden Teachers. Throughly colonized and ready to use. Now I have done a lot of research and it's been confusing, and some times useful. How ever my searches did not reveal the question(s), I have.

I'm using an old spore syringe. Here is what I plan to do. 1. Suck up alcohol in the syringe then squirt out the alcohol. 2. Throughly agitate the lc. 3. Then flame the syringe 4. Wipe syringe with alcohol wipe. 5. Insert the needle into the silicone injection port on the lc. 6. Suck up 10ml of all the globes that I can. 7. Pull out syringe. 8. Wipe with alcohol pad again. 9. Inject it all into the grain jar. 10. Pull out(thats what she said lol). 11. Suck up alcohol again in the syringe and start all the steps again.

OK I hope that explains my procedure well. But I have doubts. 1. Should I suck up alcohol each time, or will that kill the mycelium. 2. Should I flame the needle when inserting in to the jars every time or will the heat kill the myc on the out of the needle because it is too hot. 3. After flaming I always wipe with and alcohol pad, but I don't think that cools it down enough. 4. The grain jars were made 14 days ago(I just bought a condo so I didn't have time to inoculate.) Knowing that the grain looks beautiful still with no contams at all, perfect moisture content but will it actually still colonize. I need some help from a pro please address each question. I have had great success but still a noob.

Thank you soo sooo much in advance .

Much luv all.


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We can't stop here...This is bat country!!!

Fear and Loathing is the greatest movie ever!!!


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OfflineVegan
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Registered: 08/16/07
Posts: 352
Last seen: 14 years, 6 months
Re: Inoculation of liquid culture [Re: joshua m]
    #7431721 - 09/20/07 10:51 AM (16 years, 4 months ago)

im no pro but 14 day old grains..me thinks not..you coull but seems like you are asking for trouble grain is cheap and if you get contams its gonna cost you more for new jars than some new grain..
just my opinion

and alcohol in a syringe???..not for me..
as far as the syringes
boil some water and suck some boiling water and expell it 3-4 times and then on the last one suck it up and let sit it the syringe till it cools,, then expel the water sterilize the needle and then move on to the lc
or
just pc the syringes with water in em and flame sterlize the needle before aspirating LC


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I came, I saw , I came back


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Invisiblejoshua m
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Registered: 07/14/07
Posts: 168
Loc: MiD wEsT
Re: Inoculation of liquid culture [Re: Vegan]
    #7436636 - 09/21/07 01:36 PM (16 years, 4 months ago)

I need a tek for inoculation of liquid 5culture some one pleasssssseeeee direct me i have been searching for hours.


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We can't stop here...This is bat country!!!

Fear and Loathing is the greatest movie ever!!!


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OfflineNickio
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Registered: 03/14/07
Posts: 321
Last seen: 13 years, 5 months
Re: Inoculation of liquid culture [Re: joshua m]
    #7436689 - 09/21/07 01:46 PM (16 years, 4 months ago)

type freaking "LC TEK" in the search, there is all kinds of posts if you actually take the time and read, but Ill give you one right now

decide how much LC you want to make, add enough sugar(you can use Karo Corn Syrup, or Honey... Id go with karo your first time) so that the weight is 4% of the weight of the water. mix togethr, and if youre using a magnetic stirrer then put ur rod in and close jar, PC for 20 min, let cool, innoc, incubate, stir twice daily, youll see myc 3-10 days later. If solution is cloudy=contam


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OfflinekingboomerDiscord
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Registered: 06/15/04
Posts: 739
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Re: Inoculation of liquid culture [Re: joshua m]
    #7436702 - 09/21/07 01:49 PM (16 years, 4 months ago)

Flame and wipe every time, it'll be fine. the cleaner, the better imo. I've used 14 day old jars before and a few of them contammed, but not many (3 out af a dozen). Just sterilize your syringe once (in a pc if you have one), I never use alcohol to sterilize my syringes, exept after flaming the needle. Hope that helped ya!


-Kingboomer


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Hole in the sky/
take me to heaven..
window in time/
through it I'll fly...

-Black Sabbath


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Invisibledumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
Re: Inoculation of liquid culture [Re: Nickio]
    #7436718 - 09/21/07 01:52 PM (16 years, 4 months ago)



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