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Shop: Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

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Offlinetonyperez420
Shaman Rasta

Registered: 11/03/04
Posts: 1,234
Last seen: 10 years, 11 months
sterilizing water for syringes
    #7424381 - 09/18/07 03:55 PM (16 years, 4 months ago)

how long should I pressure cook water that is going to be used for syringes and grain LC's?

and how many PSI's

thanks

:mushroom2::rasta:


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Invisiblemusher_420
Stranger

Registered: 08/01/05
Posts: 2,691
Re: sterilizing water for syringes [Re: tonyperez420]
    #7424390 - 09/18/07 03:59 PM (16 years, 4 months ago)

Boiling for 20 minutes has always worked for me.


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OfflineCaptainLinger
A Fungus Amongus


Registered: 05/25/07
Posts: 1,756
Last seen: 3 years, 7 months
Re: sterilizing water for syringes [Re: musher_420]
    #7424439 - 09/18/07 04:19 PM (16 years, 4 months ago)

I'd draw the water into the syringe first. Note that your syringe tubes are open to the air when they're compressed.


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Offlinetonyperez420
Shaman Rasta

Registered: 11/03/04
Posts: 1,234
Last seen: 10 years, 11 months
Re: sterilizing water for syringes [Re: CaptainLinger]
    #7424530 - 09/18/07 04:46 PM (16 years, 4 months ago)

I am going to fill my syringes with water

PC for 20 mins at 15 PSI

that looks like it will do the trick!

I hate contams... just like making sure there is no chance for contams to show up....

thanks


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InvisibleApacheShaman
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Registered: 06/27/06
Posts: 1,346
Re: sterilizing water for syringes [Re: tonyperez420]
    #7424704 - 09/18/07 05:15 PM (16 years, 4 months ago)

thats a waist of time
just boil the water as high a temp as you can and wait a few mins and suck it up into your needle. ive done it manytimes/ it never fails.


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OfflineDr_T
Random Dude
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Folding@home Statistics
Registered: 06/03/07
Posts: 1,839
Loc: Colorado
Last seen: 16 years, 4 months
Re: sterilizing water for syringes [Re: ApacheShaman]
    #7424781 - 09/18/07 05:30 PM (16 years, 4 months ago)

Sterilization isn't a waste of time.
Use the PC. You won't regret spending more time the way you may regret not spending enough.


--------------------
Roger Rabbit said: Growing mushrooms is part art, and part science, but it's not magic.


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