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Invisibleohmatic
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using old dehydated colonised mex-a agar plates
    #7422513 - 09/18/07 06:27 AM (16 years, 4 months ago)

yesterday i found some agar plates from a mex-a sclerotia project i once started and never continued.

the agar in those plates all shrunk about half an inch from the outer edges of the petri dish,
clearly due to drying out.

now i wonder, can i somehow revive that myc by simply throwing it onto a new agar plate
or what ? ive read posts about using dry mushroom tissue and basically,
this is nothing else but dry tissue right ?

so, what to do in this case ?


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OfflineRogerRabbitM
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Re: using old dehydated colonised mex-a agar plates [Re: ohmatic]
    #7422547 - 09/18/07 06:46 AM (16 years, 4 months ago)

There should have been a few stones develop. If so, cut one in half and lay the broken part down on a fresh dish. If not, it's probably not a good substrain anyway, because mex a seems to begin to form sclerotia by the time the dish is colonized.
RR


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Re: using old dehydated colonised mex-a agar plates [Re: RogerRabbit]
    #7422550 - 09/18/07 06:49 AM (16 years, 4 months ago)

oh i didnt make that one clear it seems, i transferred only the sclerotia from the original dish,
and so on and so on.


might want to take a look at the thread:
http://www.shroomery.org/forums/showflat.php/Number/5676075#5676075


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Re: using old dehydated colonised mex-a agar plates [Re: ohmatic]
    #7430778 - 09/20/07 01:08 AM (16 years, 4 months ago)

ok ill cook some agar up today.


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OfflineHotnuts
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Re: using old dehydated colonised mex-a agar plates [Re: ohmatic]
    #7435665 - 09/21/07 08:41 AM (16 years, 4 months ago)

I've had the same thing happen and just by applying the dry agar to fresh agar worked fine.


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Re: using old dehydated colonised mex-a agar plates [Re: Hotnuts]
    #7435905 - 09/21/07 09:59 AM (16 years, 4 months ago)

Quote:

Hotnuts said:
I've had the same thing happen and just by applying the dry agar to fresh agar worked fine.




are you referring to sclerotia forming mycelium or just any mycelium ?


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OfflineHotnuts
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Re: using old dehydated colonised mex-a agar plates [Re: ohmatic]
    #7436460 - 09/21/07 12:47 PM (16 years, 4 months ago)

With any mycelium.

I'd think it would work as easily with sclerotia forming species of Psilocybe's, or most of the sclerotia producers for that matter.


Edited by Hotnuts (09/21/07 12:51 PM)


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Re: using old dehydated colonised mex-a agar plates [Re: Hotnuts]
    #7439395 - 09/22/07 06:13 AM (16 years, 4 months ago)

this is how the plates look:


not all of them have shrunk to my positive surprise.

ill simply prepare some rye berry jars and throw some pieces in there.


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OfflineAndrew47
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Re: using old dehydated colonised mex-a agar plates [Re: ohmatic]
    #7443741 - 09/23/07 11:01 AM (16 years, 4 months ago)

Let us know how this goes. I have some very old agar dishes of garden giant and a couple other edibles that I'm curious if this would work with.


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