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ski
Fellow myco



Registered: 08/21/07
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What is this?? Can i case this? (PICS)
#7420148 - 09/17/07 03:26 PM (16 years, 4 months ago) |
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So today is 2 weeks after my initial inoc..... I have this B+ Jar thats about done 
and im wondering...if im going to case this does it matter that the last little bit isnt colonized, since im going to leave it in the incubator to colonize the casing?
And second, now i know this is contam, but im wondering exactly what it is? This is the best picture i could get.... strain is Orissa India..
 Everything looks swollen and discolored.... but not green
More clear updated picture of possible contam....
Edited by ski (09/17/07 05:58 PM)
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The shroomy 1
Luminous beings surround me




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Re: 2 Questions... Contam...Casing? [Re: ski]
#7420206 - 09/17/07 03:39 PM (16 years, 4 months ago) |
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Hard to tell from that pic...looks like it focused on the wall behind it
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ski
Fellow myco



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Re: 2 Questions... Contam...Casing? [Re: The shroomy 1]
#7420677 - 09/17/07 05:42 PM (16 years, 4 months ago) |
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Hows that new pic?
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The shroomy 1
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Re: 2 Questions... Contam...Casing? [Re: ski]
#7420740 - 09/17/07 05:59 PM (16 years, 4 months ago) |
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Quote:
ski said: Hows that new pic?
Better, but still........... It appears to behave like trich, but that's not common in a jar. Green mold usually is white before it turns green. I do know that it sure is contamination. The fact that the healthy mycelium is sectoring at that border is a clear cut sign that bad things are happening.
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wutang
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Re: 2 Questions... Contam...Casing? [Re: The shroomy 1]
#7420766 - 09/17/07 06:05 PM (16 years, 4 months ago) |
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well i see green and i dont like what i see  DONT CASE UNLESS ITS 100% COLONIZED
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cliffton hanger
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Re: 2 Questions... Contam...Casing? [Re: ski]
#7420790 - 09/17/07 06:12 PM (16 years, 4 months ago) |
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always wait for full colinization, or risk total loss. the bottom pics don,t look good, try half pintjar
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ski
Fellow myco



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the bottom pics are my suspected contam, well i know its contam just not sure what.... the top is b+....what would you say for time on that... my guess is i can case about friday?
Oh yeah and when black mold forms does it spread or is it like a tiny spec? I suspect black mold in a contamed jar but its a spec the size of a pea thats been there for about 4 days and the myc looks really thick and healthy, im starting to think its just part of my brf jars... The same for trich, i have a jar that has a green dot and it doesnt appear to be growing, its been a week, and now the myc is starting to grow over it..... so could that green spot be part of the brf as well if the myc is starting to grow over it and the green hasnt spread?
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The shroomy 1
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Re: 2 Questions... Contam...Casing? [Re: ski]
#7420960 - 09/17/07 07:00 PM (16 years, 4 months ago) |
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Black mold spreads early and extremely fast! It looks like white mycelium but kind of like cobweb then turns black. I one time thought it was SUPER MYCELIUM. LOL! Then 2 days later my entire jar turned from white to black. If you inoculate and 2 days later you have what you think is massive mycelial growth, it's probably black mold.
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ski
Fellow myco



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Re: 2 Questions... Contam...Casing? [Re: The shroomy 1]
#7420979 - 09/17/07 07:05 PM (16 years, 4 months ago) |
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Well its been 2 weeks to the day i have two jars i keep out of my incubation chamber just in case, and one of those is for sure contaminated... its the picture above, the other looked like it might have trich but since then the green, or what i thought was green, hasnt moved or grown. I have a few jars that have black specs near the top of the jar, near the dry verm layer and they too arent growing or anything, Most my jars are 50- 75 percent colonized with a few ahead and a few behind, i have 2 pink buffalo jars that just germinated about 5 days ago. Im just being super observative of my jars and and probably mildly paranoid. I just inoced a 4 pound rye berry bag with 8 ccs of lipa kai and i was super generous with lysol, I WONT LOSE THAT BAG!!!
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The shroomy 1
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Re: 2 Questions... Contam...Casing? [Re: ski]
#7421097 - 09/17/07 07:42 PM (16 years, 4 months ago) |
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I personally don't freak out on specs. It's when I see that my mycelium stops and forms a border that I say "Shit!". On another note, I have found out that there is no coming back from contamination. I have tried time after time to beat it, and I have given up. And yet on another note, since I built my homemade flowhood, I have yet to get contamination. (KNOCK ON WOOD!)
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ski
Fellow myco



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Re: 2 Questions... Contam...Casing? [Re: The shroomy 1]
#7421125 - 09/17/07 07:49 PM (16 years, 4 months ago) |
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I would love to get a flow hood but im not going to grow on that kind of scale seeing as how im growing in a college dorm.... i just turn off all fans and close all doors and lysol the shit out of my closet, after i empty it, and lysol about twice more, as well as outside of it, i wash my hands and spray myself with lysol as well and flame my needle and wipe with alcohol pads and so far i only have one of 12 contamed for sure............. everything i have is specs except that one jar in the pics above, but the other stuff looks like a pea sized amount of light green, i think im going to case that by itself in another FC...
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wutang
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Re: 2 Questions... Contam...Casing? [Re: ski]
#7421157 - 09/17/07 07:58 PM (16 years, 4 months ago) |
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my god man, 8cc for 4 pound bag?maybe that has something to do with it? i would only use 3cc or 4 at the most but i probably wouldnt even go to 4
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ski
Fellow myco



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Re: 2 Questions... Contam...Casing? [Re: wutang]
#7421165 - 09/17/07 08:01 PM (16 years, 4 months ago) |
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Mycopath says 6-12 cc's and i just inoced today, the contam im talking about is in my brf jars from two weeks ago... and i just looked, its about 7 cc's that went in, theres about a 1 cc air bubble...
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wutang
fungi



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Re: 2 Questions... Contam...Casing? [Re: ski]
#7421169 - 09/17/07 08:02 PM (16 years, 4 months ago) |
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wow, let us know what happens
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ski
Fellow myco



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Re: 2 Questions... Contam...Casing? [Re: wutang]
#7421182 - 09/17/07 08:04 PM (16 years, 4 months ago) |
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Well i dont understand why mycopath would say 6-12 cc's for a 4 lb bag, i would assume graham knows what hes talking about, and im not questioning you at all, im a noob..... so even if i used 6 cc's which is their minimum suggestion it would still be more than you recommend.
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wutang
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Re: 2 Questions... Contam...Casing? [Re: ski]
#7421211 - 09/17/07 08:11 PM (16 years, 4 months ago) |
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thats really strange to be honest 12 cc is crazy http://www.the-shroom-room.com/store/product.php?productid=16189&cat=262&page=1 i shot up 4 quart rye jars with 12 cc (one syringe)
mushy marts says 3 pound rye bag to add 2-3 cc's why did they tell you 6-12?...
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ski
Fellow myco



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Re: 2 Questions... Contam...Casing? [Re: wutang]
#7421218 - 09/17/07 08:13 PM (16 years, 4 months ago) |
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Instructions: First, inspect the bag for tears or punctures. If there are any, please contact us to arrange for a replacement. Towards the bottom of the bag there is a black foam disc. This is the self-healing injector site. There is a plastic sheet covering this disc that is attached to the bag. DO NOT remove this sheet, you will inject through it. Use a cotton swab soaked with rubbing alcohol to wipe the area of the injection site. This will sterilize the port for injection buy your spore or culture syringe. Unpack your spore or culture syringe and flame the needle with a lighter till the needle is glowing red, but not melting the plastic needle hub. Once it is glowing red, wipe it down to cool it. Insert this syringe into the black injection port and inject 6-12 cc’s of solution into the substrate. We recommend using no more than 12cc’s because you might over wet the substrate which could result in contamination.
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wutang
fungi



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Re: 2 Questions... Contam...Casing? [Re: ski]
#7421232 - 09/17/07 08:17 PM (16 years, 4 months ago) |
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thats seems over kill
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wutang
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Re: 2 Questions... Contam...Casing? [Re: wutang]
#7421237 - 09/17/07 08:18 PM (16 years, 4 months ago) |
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heres mushy marts instructions Locate the small injection port. Swab the site with an alcohol pad or cotton ball. Inject 2-3 cc's of solution into the substrate. If you spread the solution around faster colonization will occur. At this time place the substrate bag into a warm, dark area for incubation. The optimal temperatures will range from 82-86 degrees depending on your species; room temperature is usually fine too. Incubate for 3-4 weeks or until fully colonized with white mycelium
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ski
Fellow myco



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Re: 2 Questions... Contam...Casing? [Re: wutang]
#7421242 - 09/17/07 08:19 PM (16 years, 4 months ago) |
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Maybe it is, i really couldnt say. But seeing as how its already done, do you suggest massaging the bag to get the moisture spread around? Ive read that it will speed up colonization.. its sitting in my incubator at 84 degrees..
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