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austio_ferocious
Amateurmycologist


Registered: 07/01/05
Posts: 165
Loc: a place I call my cave.
Last seen: 16 years, 4 months
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starting pan cyan on agar, seeking the gurus please reply
#7419220 - 09/17/07 10:12 AM (16 years, 4 months ago) |
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Hi all, it's good to be back. I am going to start pan cyan tropicalis on agar. I mixed my agar and sterilized last night. I had white settled to the bottom when it had set. I suppose I just need to shake it well before pouring, but I want to make sure it's not because it hasn't mixed/dissolved properly. Secondly, I have read and read that pay cyan are more difficult to get a good isolate from. I.E. it all looks fluffy and it's difficult to find the best piece of growth to use. The agar is PDA powder from sporeworks. I need to take a minute to rave about this vendor too. I got not one but two free prints. How much do these guys ROCK!?! The other print I got was hypholoma capnoids or smoky gilled wood lover, which grown on douglas fir, can these and other wood lovers be spawned to rye berries, as this is my favorite spawn substrate, or do I need to use sawdust? I have all the pooh I could ever need and am picking up a 70 lb. bail of wheat straw. I plan to make simple 50/50 with these and case with 50/50 peat and verm. Suggestions? Questions? Comments?
Edited by austio_ferocious (09/18/07 10:33 AM)
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AFOD
Abraham


Registered: 01/25/07
Posts: 62
Loc: In the heart of mormon co...
Last seen: 16 years, 4 months
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Can anyone help on this one. I am at a loss.
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austio_ferocious
Amateurmycologist


Registered: 07/01/05
Posts: 165
Loc: a place I call my cave.
Last seen: 16 years, 4 months
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Re: starting pan cyan on agar...?s [Re: AFOD]
#7419745 - 09/17/07 01:36 PM (16 years, 4 months ago) |
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I understand pan myc is not as brilliant white as that of cubes. but how do I know what would be the best for transfer? Anyone... It seems no one knows the answer. That would be a first and frankly I don't believe it.
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AFOD
Abraham


Registered: 01/25/07
Posts: 62
Loc: In the heart of mormon co...
Last seen: 16 years, 4 months
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Bump...anyone? I am beginning to become concerned. Is PDA a suitable agar recipe for pan cyans?
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Dr_T
Random Dude




Registered: 06/03/07
Posts: 1,839
Loc: Colorado
Last seen: 16 years, 4 months
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Re: starting pan cyan on agar...?s [Re: AFOD]
#7420301 - 09/17/07 04:08 PM (16 years, 4 months ago) |
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PDY agar is used for a wide variety of fungi and other microorganisms. Seems like a good place to start...
-------------------- Roger Rabbit said: Growing mushrooms is part art, and part science, but it's not magic.
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Robo
R Series 66Y
Registered: 05/08/07
Posts: 14,861
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Edited by Robo (08/12/15 10:53 PM)
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AFOD
Abraham


Registered: 01/25/07
Posts: 62
Loc: In the heart of mormon co...
Last seen: 16 years, 4 months
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Re: starting pan cyan on agar...?s [Re: Robo]
#7422898 - 09/18/07 09:51 AM (16 years, 4 months ago) |
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Well, Austio_fercoious wiped about 4 plates with the tropicalis spores, but he had a sealed jar with some cambodian long stem spore solution which he thinks he accidentally poured over the fourth plate after wiping the trops spores on it. He had intended to pour it onto it's very own plate, but forgot to move the last plate he wiped with trops. That could be interesting. The plates look great and the agar looks very smooth. I also had some wild spores from a shaggy mane that I found in the woods, which I wiped onto a plate at his house while he was at it. He saved plenty of agar from this batch for transfer plates. Though we still dont know what part of the pan myc would be best to transfer. I guess we may wait for them to fruit (poorly) invitro on the agar and clone those fruits to get a good fruiting strain, as RR seggests in other posts, though we were hoping for a quicker identification for isolation. He also saved most of the trop print for a syringe and other plates. Since it so small, he wants to try a few things so that he can get a good mother culture. Pics will hoefully be available soon. Yes, this is a group effort. I with my edibles and novelties, and he with his others, He keeps his at his place and let's me use his lab for my spawning. Austio you ROCK brother. I mean that in the sense of comraudery and fellowship as we are not blood related. Would you consider the handshake of brotherhood with me?
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austio_ferocious
Amateurmycologist


Registered: 07/01/05
Posts: 165
Loc: a place I call my cave.
Last seen: 16 years, 4 months
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Re: starting pan cyan on agar...?s [Re: AFOD]
#7422937 - 09/18/07 10:10 AM (16 years, 4 months ago) |
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I would bleed for you brother. What do you think about those pan/cambodians? Think they'll mutate into an ultra potent cube, or a larger growing pan? Fuck they'll probably kill each other off. Oh well. WE'll see what happens. maybe we can seperate them. Anyone know how to do that? Y'know brother it seems that we be talkin to ourselves most of the time here. I remember a time when someone would ask a question and people would jump on it. Now it's like being stranded on the freeway... ZOOOM.... ZOOOOOM, Er, excuse... Zoooom! I feel like we're going to choke this one if we don't get some advise soon. Good advise, like RR or Hyphea or Mj, y'know, one of the experts. Come over later we'll become brothers in blood.
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dysphoria
lost soul


Registered: 06/04/03
Posts: 1,651
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selecting a good strand of growth for isolation is most likely not going to become apparent until you transfer to another petri dish or two. oftentimes it takes a few transfers before truly rhizomorphic growth shows up. when dealing with pan cyan cultures on agar, ive found that selecting the best section to transfer is usually the fastest growth, and also the wispiest. the further the mycelium is getting from the center, the better the growth, naturally.
as for your agar, you're fine, sometimes there is sediment...hell, sometimes i have large chunks of ...something that didnt dissolve completely, or melt back down or whatever...and the petris always work out just fine.
and to be honest, im not sure you'll ever see an in vitro pin off of pan cyan. could be wrong, but its one of those picky species that absolutely requires a casing layer.
i dont think i covered all your questions, but if you two have any more stuff or need clarification, id be glad to help.
and sorry for lack of pan cyan agar pictures, long standing practice to not photograph anything but oysters. =)
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