Home | Community | Message Board

Original Seeds Store
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Capsules   Original Sensible Seeds High THC Strains   North Spore Bulk Substrate   Bridgetown Botanicals CBD Concentrates   PhytoExtractum Kratom Powder for Sale   Mushroom-Hut Grow Bags

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlineaustio_ferocious
Amateurmycologist


Registered: 07/01/05
Posts: 165
Loc: a place I call my cave.
Last seen: 16 years, 4 months
starting pan cyan on agar, seeking the gurus please reply
    #7419220 - 09/17/07 10:12 AM (16 years, 4 months ago)

Hi all, it's good to be back.
I am going to start pan cyan tropicalis on agar. I mixed my agar and sterilized last night. I had white settled to the bottom when it had set. I suppose I just need to shake it well before pouring, but I want to make sure it's not because it hasn't mixed/dissolved properly.
Secondly, I have read and read that pay cyan are more difficult to get a good isolate from. I.E. it all looks fluffy and it's difficult to find the best piece of growth to use.
The agar is PDA powder from sporeworks.
I need to take a minute to rave about this vendor too. I got not one but two free prints. How much do these guys ROCK!?!
The other print I got was hypholoma capnoids or smoky gilled wood lover, which grown on douglas fir, can these and other wood lovers be spawned to rye berries, as this is my favorite spawn substrate, or do I need to use sawdust?
I have all the pooh I could ever need and am picking up a 70 lb. bail of wheat straw. I plan to make simple 50/50 with these and case with 50/50 peat and verm.
Suggestions? Questions? Comments?


Edited by austio_ferocious (09/18/07 10:33 AM)


Extras: Filter Print Post Top
OfflineAFOD
Abraham
Male

Registered: 01/25/07
Posts: 62
Loc: In the heart of mormon co...
Last seen: 16 years, 4 months
Re: starting pan cyan on agar...?s [Re: austio_ferocious]
    #7419576 - 09/17/07 12:20 PM (16 years, 4 months ago)

Can anyone help on this one. I am at a loss.


Extras: Filter Print Post Top
Offlineaustio_ferocious
Amateurmycologist


Registered: 07/01/05
Posts: 165
Loc: a place I call my cave.
Last seen: 16 years, 4 months
Re: starting pan cyan on agar...?s [Re: AFOD]
    #7419745 - 09/17/07 01:36 PM (16 years, 4 months ago)

I understand pan myc is not as brilliant white as that of cubes. but how do I know what would be the best for transfer?
Anyone...
It seems no one knows the answer.
That would be a first and frankly I don't believe it.


Extras: Filter Print Post Top
OfflineAFOD
Abraham
Male

Registered: 01/25/07
Posts: 62
Loc: In the heart of mormon co...
Last seen: 16 years, 4 months
Re: starting pan cyan on agar...?s [Re: austio_ferocious]
    #7420272 - 09/17/07 03:57 PM (16 years, 4 months ago)

Bump...anyone? I am beginning to become concerned. Is PDA a suitable agar recipe for pan cyans?


Extras: Filter Print Post Top
OfflineDr_T
Random Dude
Male User Gallery


Folding@home Statistics
Registered: 06/03/07
Posts: 1,839
Loc: Colorado
Last seen: 16 years, 4 months
Re: starting pan cyan on agar...?s [Re: AFOD]
    #7420301 - 09/17/07 04:08 PM (16 years, 4 months ago)

PDY agar is used for a wide variety of fungi and other microorganisms. Seems like a good place to start... :shrug:


--------------------
Roger Rabbit said: Growing mushrooms is part art, and part science, but it's not magic.


Extras: Filter Print Post Top
InvisibleRobo
R Series 66Y
Registered: 05/08/07
Posts: 14,861
Re: starting pan cyan on agar...?s [Re: austio_ferocious]
    #7420315 - 09/17/07 04:12 PM (16 years, 4 months ago)

   


Edited by Robo (08/12/15 10:53 PM)


Extras: Filter Print Post Top
OfflineAFOD
Abraham
Male

Registered: 01/25/07
Posts: 62
Loc: In the heart of mormon co...
Last seen: 16 years, 4 months
Re: starting pan cyan on agar...?s [Re: Robo]
    #7422898 - 09/18/07 09:51 AM (16 years, 4 months ago)

Well, Austio_fercoious wiped about 4 plates with the tropicalis spores, but he had a sealed jar with some cambodian long stem spore solution which he thinks he accidentally poured over the fourth plate after wiping the trops spores on it. He had intended to pour it onto it's very own plate, but forgot to move the last plate he wiped with trops. That could be interesting. The plates look great and the agar looks very smooth.
I also had some wild spores from a shaggy mane that I found in the woods, which I wiped onto a plate at his house while he was at it.
He saved plenty of agar from this batch for transfer plates. Though we still dont know what part of the pan myc would be best to transfer. I guess we may wait for them to fruit (poorly) invitro on the agar and clone those fruits to get a good fruiting strain, as RR seggests in other posts, though we were hoping for a quicker identification for isolation.
He also saved most of the trop print for a syringe and other plates.
Since it so small, he wants to try a few things so that he can get a good mother culture. Pics will hoefully be available soon.
Yes, this is a group effort. I with my edibles and novelties, and he with his others, He keeps his at his place and let's me use his lab for my spawning. Austio you ROCK brother. I mean that in the sense of comraudery and fellowship as we are not blood related. Would you consider the handshake of brotherhood with me?


Extras: Filter Print Post Top
Offlineaustio_ferocious
Amateurmycologist


Registered: 07/01/05
Posts: 165
Loc: a place I call my cave.
Last seen: 16 years, 4 months
Re: starting pan cyan on agar...?s [Re: AFOD]
    #7422937 - 09/18/07 10:10 AM (16 years, 4 months ago)

I would bleed for you brother. What do you think about those pan/cambodians? Think they'll mutate into an ultra potent cube, or a larger growing pan? Fuck they'll probably kill each other off. Oh well. WE'll see what happens. maybe we can seperate them.
Anyone know how to do that?
Y'know brother it seems that we be talkin to ourselves most of the time here. I remember a time when someone would ask a question and people would jump on it. Now it's like being stranded on the freeway... ZOOOM.... ZOOOOOM, Er, excuse... Zoooom!
I feel like we're going to choke this one if we don't get some advise soon. Good advise, like RR or Hyphea or Mj, y'know, one of the experts.
Come over later we'll become brothers in blood.


Extras: Filter Print Post Top
Invisibledysphoria
lost soul

Folding@home Statistics
Registered: 06/04/03
Posts: 1,651
Re: starting pan cyan on agar...?s [Re: austio_ferocious]
    #7423351 - 09/18/07 11:46 AM (16 years, 4 months ago)

selecting a good strand of growth for isolation is most likely not going to become apparent until you transfer to another petri dish or two. oftentimes it takes a few transfers before truly rhizomorphic growth shows up. when dealing with pan cyan cultures on agar, ive found that selecting the best section to transfer is usually the fastest growth, and also the wispiest. the further the mycelium is getting from the center, the better the growth, naturally.

as for your agar, you're fine, sometimes there is sediment...hell, sometimes i have large chunks of ...something that didnt dissolve completely, or melt back down or whatever...and the petris always work out just fine.

and to be honest, im not sure you'll ever see an in vitro pin off of pan cyan. could be wrong, but its one of those picky species that absolutely requires a casing layer.

i dont think i covered all your questions, but if you two have any more stuff or need clarification, id be glad to help.

and sorry for lack of pan cyan agar pictures, long standing practice to not photograph anything but oysters. =)


--------------------


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Left Coast Kratom Buy Kratom Capsules   Original Sensible Seeds High THC Strains   North Spore Bulk Substrate   Bridgetown Botanicals CBD Concentrates   PhytoExtractum Kratom Powder for Sale   Mushroom-Hut Grow Bags


Similar ThreadsPosterViewsRepliesLast post
* Re: -The difficulty of Pan Cyans?- Anonymous 4,027 2 12/12/99 11:37 AM
by Anonymous
* Pan. Cyans. Grow... Boppity604 5,317 12 08/08/02 05:01 AM
by Raadt
* how much experience is needed to grow Pan Cyans? Psike 5,460 6 06/14/01 05:58 AM
by auto59009
* Pan. Cyan Cultivation ???? J_Twista 5,478 9 08/02/01 07:25 AM
by ralphster44
* Pan Cyan Fruiting? PoppaWoody 4,705 6 06/24/01 10:22 PM
by ralphster44
* Pan Cyans in casings, are they ready to fruit? PoppaWoody 2,056 5 06/28/01 05:13 PM
by Champion des Champignons
* Will the real "Pan Cyan" please stand up? Mr. G 4,668 7 05/13/02 05:47 AM
by Workman
* Can I Grow Pan Cyans on MMGG or Hawk Magic? WhAcKeD 952 3 08/05/02 03:41 AM
by Waldarbeiter

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,899 topic views. 19 members, 158 guests and 45 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.014 seconds spending 0.003 seconds on 12 queries.