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OfflineHankHill
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Registered: 04/07/06
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Last seen: 16 years, 1 month
screwy Liquid Culture, and question on my grow
    #7419198 - 09/17/07 10:01 AM (16 years, 4 months ago)

I took part of a healthy succesful LC which has given me a good colonization in a jar so far and put put it in another jar of honey/water prepared normally.

The mycelium grew, but it is behaving very oddly and it is all connected at the very top of the jar rather than floating in the bottom.

When I shake it up I notice little black stringy particulate matters interspersed with the otherwise healthy looking white mycelium.

What is this? is it contaminated?

the first LC i tried to do got contamined and it was just a complete murky ugly pool, but this is clearly mycelium and if not for the strings of black it would look completely normal.

the strings are not in any way prominent but they seem to be 'fiber strands' or something.

do you propogate liquid culture by taking some from one jar and injecting it into a new jar where it will grow, is this the right way of doing it?

now, also, will even the liquid in the jars that does not have visible mycelium, i.e. just the honey water, still also work for colonizing jars, or do you have to suck up as much of the mycelium as possible into the syringe

onto my grow,

i inoculated from spore syringe like 3 weeks ago, and after about 4 or 5 days i saw rapid growth, however now the jars seem to be at a stand still with only 1/3 of them looking very close to being fully colonized, yet they have looked this way, very close, for an entire week without showing any progress

there are parts of the jars that do not have mycelium in them, however these parts are small

the other jars are not nearly as developed

am i to be patient, will they colon9ize and pin in time, or is there a problem with the growth ? what if they start pinning yet do not have full even colonization of mycelium across the entire surface of the cake, just put them in the chamber anyway?

my LC jars are going to probably outgrow these ones and be pinned at roughly the same time as these ones do.... but i shot the LC jars up with generous amounts.

and finally, wal-mart did not have the drill i had my eye on, but thought i found a better one for much cheaper, the issue was that it makes holes that are so very tiny, that i do not know if these holes will give my fruiting chamber adequate ventilation.

if you took an ink pin and stabbed holes in things, that is about how big these holes are.

no doubt, i can simply fan fresh air into the chamber each day though.

i would post pictures but i can't find my plug for my camera phone.


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OfflineDr_T
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Re: screwy Liquid Culture, and question on my grow [Re: HankHill]
    #7419229 - 09/17/07 10:16 AM (16 years, 4 months ago)

Hard to say if it's a contam. Try shooting up a jar.

Stalled jars- what type of substrate?

Drills... can't you change the bit?


--------------------
Roger Rabbit said: Growing mushrooms is part art, and part science, but it's not magic.


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OfflineHankHill
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Last seen: 16 years, 1 month
Re: screwy Liquid Culture, and question on my grow [Re: Dr_T]
    #7419919 - 09/17/07 02:28 PM (16 years, 4 months ago)

drills..... it is a very small drill/screwdriver and it doesn't come with other bits

unfortunately the LC jar i considered healthy seems to be developing some yellow coloring, i suppose this is mold and not just the effects of the lingering honey?

i am going to leave it alone until it gets a lot more prominent but i threw 2 straggler jars away that i shot up with LC because they clearly turned yellow, i assumed it was due to poor inoculation procedures rather than a bum LC

the substrate is BRF/verm

when i made this culture i took the honey and water and put it in a jar, then boiled an hour or so, then inoculated in a clean glove box

why should these jars be contaminated and why should the liquid culture be contaminated?


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OfflineDr_T
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Re: screwy Liquid Culture, and question on my grow [Re: HankHill]
    #7419984 - 09/17/07 02:44 PM (16 years, 4 months ago)

So... you don't pressure cook your LC?
I've never tried just boiling it.

I doubt that a yellow color is due to mold. Mold will show signs of growth, it looks like mycelium. I wouldn't throw out a jar until I knew it was bad.

Haven't tried BRF/verm, can't really say much about that.


--------------------
Roger Rabbit said: Growing mushrooms is part art, and part science, but it's not magic.


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OfflineHankHill
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Last seen: 16 years, 1 month
Re: screwy Liquid Culture, and question on my grow [Re: Dr_T]
    #7420096 - 09/17/07 03:14 PM (16 years, 4 months ago)

really

a very distinct yellow, okay to leave it be and watch it grow?

i was about to boil/kill the current LC and make another one with spores,

how will i know whether it is contaminated or not then, if it has a lot of good healthy white mycelium, but then a lot of yellow colors are coming in?

i'm leaving the 3-5 (i forget exact #) of LC do their thing, the others haven't really started growth yet but one i did a few days earlier is showing the yellows ......

i did put a LOT of solution in, between 1 and 2 ml per jar. (or 1 or 2 measurement numbers, with the giant syringes being divided by 10 of them, i'm not sure if it's ml or not) so the yellow could be from the honey water ?


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OfflineDr_T
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Re: screwy Liquid Culture, and question on my grow [Re: HankHill]
    #7420112 - 09/17/07 03:18 PM (16 years, 4 months ago)

Best way to tell if it's contaminated is by shooting up a jar of grain.


--------------------
Roger Rabbit said: Growing mushrooms is part art, and part science, but it's not magic.


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OfflineHankHill
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Last seen: 16 years, 1 month
Re: screwy Liquid Culture, and question on my grow [Re: Dr_T]
    #7421420 - 09/17/07 09:00 PM (16 years, 4 months ago)

does anyone else have an opinion on this

i am leaving this jar going, and what i think will happen is that the white mycelium will overtake and obscure the yellow colors, but if the yellow is contamination, this could be a problem right?

if you open a contaminated jar it runs health and sanitation issues, right?

i'm not sure what the yellow is, it does not grow in the same manner as th emycelium and i'm not sure if it 'grows' or not?

the only thing i can think is that it is the honey/water and that when the mycelium grows on the substrate this separates it some from the honey/water which there happens to be a lot of and therefore the substrate gets coated with a yellow hue

this is what i HOPE it is

i am making another LC with a different strain and will experiment on a jar to see how it goes

and then will also try my reishi LC on a jar to see if i can't narrow down some things and see if it's an issue of the particular syringe, or an issue of sanitation/preparation (and i KNOW the boil gets it very very very hot and my glove-box is thoroughly Ousted each time so ???) it can't be the glove box since the jars shot with straight spore syringe are ALL fine, all of them,


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OfflineHankHill
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Registered: 04/07/06
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Last seen: 16 years, 1 month
Re: screwy Liquid Culture, and question on my grow [Re: HankHill]
    #7427357 - 09/19/07 09:31 AM (16 years, 4 months ago)

i belive the healthy white mycelium will engulf the yellow coloration

if it is contamination, will the mycelium then therefore kill it ?

basically, do you only judge contamination based upon how the jar looks when fully colonized
if it colonizes fully, fully white with no more of this yellow to be seen, do i assume it is good to fruit?


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OfflineHankHill
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Registered: 04/07/06
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Last seen: 16 years, 1 month
Re: screwy Liquid Culture, and question on my grow [Re: HankHill]
    #7429010 - 09/19/07 04:55 PM (16 years, 4 months ago)

i could really use some more opinions on this

my grow's entire success might depend upon the liquid culture

i flipped most of my slow cakes upside down and if i don't see anything happen by friday i'm thinking of putting them in the fruiting chamber

is it better to case stalled cakes than to just put them in the chamber as they are ?

how much would casing with something other than JUST vermiculite improve success ?

the yellow is troubling honestly, it could easily be mold.


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