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Offlineanthony034
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Registered: 05/24/07
Posts: 307
Loc: International Waters
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LC noob
    #7409562 - 09/14/07 02:46 PM (16 years, 4 months ago)

i attempted a liquid culture using the karo tek 3 days ago.
what should i be looking for positive or negative? it seems fairly clear until i shake a little, then i can see a large swirl in the jar. mycelium? contam? it is not cloudy, more of an isolated swirl in the water. would take a picture but nothing will be visible.


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YES WE CAN




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OfflineAFOD
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Registered: 01/25/07
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Re: LC noob [Re: anthony034]
    #7409864 - 09/14/07 03:51 PM (16 years, 4 months ago)

I'm a noob, but I have do have some experience with lc of green mold.:( does it look like a fluffy little cloud? kinda puffy stringy? Whether it's mushroom myc or mold is difficult to tell. I had a few jars that I thought had great myc growth, but when I spawned to grain, it grew out into mold... Anyone have a better way to tell the difference between mold and good myc in an lc?
Also it sounds like you inoculated the lc with spores?


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Offlineanthony034
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Re: LC noob [Re: AFOD]
    #7409868 - 09/14/07 03:51 PM (16 years, 4 months ago)

Quote:

AFOD said:
I'm a noob, but I have do have some experience with lc of green mold.:( does it look like a fluffy little cloud? kinda puffy stringy? Whether it's mushroom myc or mold is difficult to tell. I had a few jars that I thought had great myc growth, but when I spawned to grain, it grew out into mold... Anyone have a better way to tell the difference between mold and good myc in an lc?
Also it sounds like you inoculated the lc with spores?




yes. inoculated with spore solution.


--------------------
YES WE CAN




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OfflineAFOD
Abraham
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Registered: 01/25/07
Posts: 62
Loc: In the heart of mormon co...
Last seen: 16 years, 4 months
Re: LC noob [Re: anthony034]
    #7409990 - 09/14/07 04:21 PM (16 years, 4 months ago)

I think I'm going to isolate on agar before I do another lc. I have yet to have a successful lc from spores. I really wish other more experienced people would chime in and maybe offer some better ways to distinguish mold from mushy in an lc....
Uh, Hem... RR where you at?


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OfflineDr_T
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Re: LC noob [Re: AFOD]
    #7410201 - 09/14/07 05:11 PM (16 years, 4 months ago)

Best way is to shoot up a jar of grain with it. Or look at it under a microscope, maybe. But there's no way to really tell by looking through the side of the jar.


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Roger Rabbit said: Growing mushrooms is part art, and part science, but it's not magic.


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