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Offlinejoekenorer
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Registered: 05/22/07
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Opinions and ideas greatly needed on this tek...
    #7403828 - 09/13/07 05:31 AM (16 years, 4 months ago)

Alright.  I am no n00b to cubensis mushrooms (nearly a decade of eating them), but I am a n00b to growing them.  I know it's not recommended but I'm winging it on my first tek.

I've been surfing and participating in the shroomery for some time now    (not as long as some of you, though) and have decided to try my hand with BlackKow manure and a colonized cow patty.  I am going to explain what I've done thus far and would like some comments or tips.

First off, sterilization and PCing goes without saying because I've done it to the extreme on all items mentioned. 
I picked up a patty that had a couple small cubes on it about 2 weeks ago and put it in a clear rubbermaid box with two 3/8" holes in it.  Myceleum growth was good for the initial week, but it later slowed.  Two days later a separate chunk of the poo began to colonize nicely, but the chunk was small.  Tonight I took all the poo and crumbled it into a 32 oz Gatorade bottle.  It filled it about an inch.  I then added the BlackKow manure up to about where the top of the label would be (had I not removed it).  I shook this around a bit to make sure everything was distributed fairly even.  I added about 4 oz of bottled water to moisten it a little more than it was, because despite its moisture content I could not squeeze any out of the BlackKow (there is no standing water at the bottom right now).  I cut a hole in the cap about 1" in diameter, then put tyvek under the cap and over it and tied it down with a rubber band. 

Now, from what I've understood thus far, this will hypothetically colonize as normal.

What I want is your opinion on this tek.  Do you think it will work?  Why/why not?  What should I definitely do or change?  What could I do to improve what I have?  Finally and most importantly, what the hell do I do next?  Such as storage area and conditions.

The cubes in my field look much more like the Golden Teachers I see on here than any other cubensis, so I feel like that's what they are.  I don't know if this helps any, but there you go. 

Please, any help or opinions would be GREATLY appreciated because I haven't seen anymore shrooms in my field since I took that patty.  I fear the season is nearing an end here in NW Florida!  (I've never found them growing past September here).


Thanks :bowdown:


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My favorites are weeping willows, which aren't really weeping at all. They're very wispy, witty and will dance in the breeze with you. Nothing like a tree that wants to dance with you. Although it doesn't like its thin limbs being pulled at all, it absolutely LOVES it when you walk through them, letting them gently slide over your face and shoulders. If you're naked, the willow considers it to be sex. It will orgasm on your mind and you will blow dream chunks into outer space. All very fun until your neighbor sees you.                                    -The Joekenorer


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Offlinexhooliganx
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Re: Opinions and ideas greatly needed on this tek... [Re: joekenorer]
    #7404628 - 09/13/07 02:08 PM (16 years, 4 months ago)

well sterilizing the manure was a horrible idea it'll most likely contam because of that. black kow is also not a highly recommended substrate. besides that I don't really know why you used tyvek on the lid when you have contams in/on the cowpoo that is partialy colonized unless you sterilized that(but then your project is done). i'm sure there are plenty of other flaws, but these should be enough to ruin your project. I think your best but would have been to try to clone one of the wild cubes. it would require agar work most likely, but otherwise you're going to have to get a print or a syringe from a vendor.


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OfflineDr_T
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Re: Opinions and ideas greatly needed on this tek... [Re: xhooliganx]
    #7406098 - 09/13/07 08:17 PM (16 years, 4 months ago)

I approve of winging it- but read the teks, and follow one until you get success. Then wing it, and post pics. If nobody ever tried anything new, there'd be no progress.


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Roger Rabbit said: Growing mushrooms is part art, and part science, but it's not magic.


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Offlineburmesepsylicibe
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Re: Opinions and ideas greatly needed on this tek... [Re: Dr_T]
    #7406349 - 09/13/07 08:59 PM (16 years, 4 months ago)

Only way I could see you getting something a clean strain off of a wild mushroom would be cloning.

look up cloning LC

Look up pasteurization

BlackKow mushroom compost is spent substrate from commercial growers. thus all of the good nutes have been used. they suggest its use for plants.

Maybe take another cow patty from the field that has no mushroom coming out. get some vermiculite. Mix the vermiculte in for consistency. probably 50/50. bring to field capacity. pastuerize. inoculate. incubate. look up casing.

best of luck


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one of these was an LC
Which do you think it was?

deformedreality said:
.. besides the dead body of that pig i hid under the perlite.. he was snooping around too much ;]


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Offlinexhooliganx
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Re: Opinions and ideas greatly needed on this tek... [Re: burmesepsylicibe]
    #7408269 - 09/14/07 09:26 AM (16 years, 4 months ago)

even if you decided to clone you should really consider getting some agar. if you get some dirty stuff in it you can take good mycelium and transfer it to another dish and have a good clean clone.

cloning homegrown vs wild grown is very different because of higher contamination vectors. if you do clone to lc then do a test jar of grain or a test cake. you generally can't tell if your lc is contaminated untill it colonizes grain


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Offlinejoekenorer
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Re: Opinions and ideas greatly needed on this tek... [Re: xhooliganx]
    #7412010 - 09/15/07 04:04 AM (16 years, 4 months ago)

Thanx guys, but I was really wanting to know...what next?  You see where I'm at.  Right now on the very top of the poo myceleum has started in a spot the size of, say, an average pipe screen.  Its on top of my hot water heater in a very small closet where the temp stays at a constant 80-88 degrees.  The surface its sitting on does not get uncomfortably hot at all, so I'm not worried about contact burn.  This speck of growth is my only hope for out of season cubensis. 
I am hoping for at least one cap, god just one, so I can get a spore print and do it properly.  What can I do from here on to best insure this stays alive and well?  I'd take a picture, but your imaginations could easily paint a better image than my shitty $75 Polaroid digital camera ever will.
Thats the information and advice I'm really looking for.  Once again, thanks.:bowdown:


--------------------
My favorites are weeping willows, which aren't really weeping at all. They're very wispy, witty and will dance in the breeze with you. Nothing like a tree that wants to dance with you. Although it doesn't like its thin limbs being pulled at all, it absolutely LOVES it when you walk through them, letting them gently slide over your face and shoulders. If you're naked, the willow considers it to be sex. It will orgasm on your mind and you will blow dream chunks into outer space. All very fun until your neighbor sees you.                                    -The Joekenorer


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Offlinejoekenorer
The Joekenorer
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Registered: 05/22/07
Posts: 626
Loc: Pensacola, FL.
Last seen: 1 year, 6 months
Re: Opinions and ideas greatly needed on this tek... [Re: joekenorer]
    #7412013 - 09/15/07 04:10 AM (16 years, 4 months ago)

And I'm using BlackKow pure manure compost, not mushroom compost. If that what you meant anyway, perhaps I am misunderstanding.

I say this only to clarify. Respond how you like but lets not make it the central subject of the thread. Like I said, I'm in a bad way right now hoping for this life-forms safety and I'm hoping thats where you guys come in.


--------------------
My favorites are weeping willows, which aren't really weeping at all. They're very wispy, witty and will dance in the breeze with you. Nothing like a tree that wants to dance with you. Although it doesn't like its thin limbs being pulled at all, it absolutely LOVES it when you walk through them, letting them gently slide over your face and shoulders. If you're naked, the willow considers it to be sex. It will orgasm on your mind and you will blow dream chunks into outer space. All very fun until your neighbor sees you.                                    -The Joekenorer


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Invisibleorchidfanatic
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Re: Opinions and ideas greatly needed on this tek... [Re: joekenorer]
    #7412074 - 09/15/07 05:27 AM (16 years, 4 months ago)

you should really look into agar . you can take a spec of this mycelium you have and put it on agar .. let it grow out check for cotamination, take a spec of mycelium from that petri dish and transfer to a new agar plate.. repeat until its clean of cotamination
then transfer a wedge of this agar into some kind of substrate.
read the teks you will get ideas of what to do


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