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OfflineCaptainObvious
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Registered: 08/09/07
Posts: 11
Last seen: 15 years, 10 months
Can LC be inoculated with slurry?
    #7402228 - 09/12/07 07:58 PM (16 years, 4 months ago)

My friend wants to try his hand at cloning. He doesn't want to use agar yet, mainly because he doesn't have any, but he does have the supplies to do LC.

He's heard of "shroom water" inoculant - taking the inside of the stem of a fresh fruit, and blending it to a slurry in sterilized water. He knows it's not a "perfect" isolation technique (like "sectoring on agar"), but he's heard that it's very effective.

So for his next spawn run, he plans to use "shroom water". It occurred to him that instead of using the "shroom water" directly, he could probably use it to start an LC, which he would then use as his "master culture".

He's pretty sure it will work, so he's really wondering if anyone out there has tried it, and what kind of success they had. He also said he'd appreciate some myco-porn, if available.

Thanks in advance.


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Offlinexhooliganx
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Registered: 03/09/06
Posts: 900
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Re: Can LC be inoculated with slurry? [Re: CaptainObvious]
    #7402271 - 09/12/07 08:09 PM (16 years, 4 months ago)

um how about instead of this "shroom water" you cut out a piece of the inside of a stem with a flamed scalple and drop that into the lc?

another route is to do a biopsy of the stem. suck up some sterile lc liquid shove the needle through a stem that has had the outer layer cut back and cleaned with iodine. shoot the stem chunk that's inside the needle into the lc.

theres a lot of posts about this kinda stuff search lc cloning


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OfflineCaptainObvious
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Registered: 08/09/07
Posts: 11
Last seen: 15 years, 10 months
Re: Can LC be inoculated with slurry? [Re: xhooliganx]
    #7402381 - 09/12/07 08:35 PM (16 years, 4 months ago)

You're right. Did just what you said, and found this.

So I guess, mods, you could move/close this post - looks like the above link has it pretty well covered.

Thanks.


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Offlinexhooliganx
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Registered: 03/09/06
Posts: 900
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Re: Can LC be inoculated with slurry? [Re: CaptainObvious]
    #7403097 - 09/12/07 11:07 PM (16 years, 4 months ago)

remember that you actualy have to get a core biopsy and that just shoving the needle through might not cut it(no pun intended). if it doesn't try to push a little and twist, push a little more twist. etc etc. also larger gauge needles help


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InvisibleZiggen
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Registered: 06/27/05
Posts: 651
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Re: Can LC be inoculated with slurry? [Re: CaptainObvious]
    #7403146 - 09/12/07 11:21 PM (16 years, 4 months ago)

Quote:

CaptainObvious said:
You're right.  Did just what you said, and found this.





Great tek. :thumbup:


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