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timmiezown
Stranger

Registered: 09/02/07
Posts: 29
Last seen: 16 years, 2 months
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Noob Questions
#7398406 - 09/11/07 08:45 PM (16 years, 4 months ago) |
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Ok two questions. before i start listing them i'd liek to thank you for taking the time to help me out.
1) Is 30 minutes of pressure cooking @ 15psi enough to get rid of almost all of the bacteria therefore making it sterile?
2) Can you mix strains when innoculating PF substrate jars? I filled 2 holes and i ran out of one strain so i just put a different strain in the other two. (for those who are wondering i overshot the syringe, yes i know its noob, my first time btw. the strains were john allen and b+)
Thanks for your time
*edit
one more question. I freaked out about being sterile and i decided to clean the inside of a garbage bag that i stored the jars to inoculate so i cleaned the inside with rubbing alcohol. assuming that it evaporates, nothing bad should come of this, correct?
Edited by timmiezown (09/11/07 08:51 PM)
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Dr_T
Random Dude




Registered: 06/03/07
Posts: 1,839
Loc: Colorado
Last seen: 16 years, 4 months
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1) I use 30 mins for half-pint jars, 60 for quarts. YMMV.
2) That won't cause problems. It's probably not the best option, but it's ok. As you said, it was a noob thing. Syringes take a little practice.
-------------------- Roger Rabbit said: Growing mushrooms is part art, and part science, but it's not magic.
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timmiezown
Stranger

Registered: 09/02/07
Posts: 29
Last seen: 16 years, 2 months
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Re: Noob Questions [Re: Dr_T]
#7398432 - 09/11/07 08:52 PM (16 years, 4 months ago) |
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thanks for the reply. what does ymmv mean?
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veilbreaker
Sour girl


Registered: 06/12/07
Posts: 528
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"Your mileage may vary"
-------------------- People seem not to see that their opinion of the world is also a confession of their character. ~Ralph Waldo Emerson
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Dr_T
Random Dude




Registered: 06/03/07
Posts: 1,839
Loc: Colorado
Last seen: 16 years, 4 months
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re: cleaning bag with alcohol. Not a problem, but avoid flames and sparks. Having a bag full of alcohol fumes explode in your face wouldn't be fun, and might very well require telling a story to somebody in a uniform...
-------------------- Roger Rabbit said: Growing mushrooms is part art, and part science, but it's not magic.
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allreadyused
The Liquor



Registered: 09/10/07
Posts: 480
Loc: Trailer Park, Nova Scotia
Last seen: 8 years, 23 days
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30 is fine but remember to wait till the pressure reaches 15 psi before you start timing. If you get syringes from a good vendor or prepare your own from a print you only need about 1cc per jar.
-------------------- Everything I say is for entertainment. Fuck the ASPCA
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timmiezown
Stranger

Registered: 09/02/07
Posts: 29
Last seen: 16 years, 2 months
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Re: Noob Questions [Re: Dr_T]
#7398654 - 09/11/07 09:45 PM (16 years, 4 months ago) |
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once again thank you for clearing up everything.
haha funny story i poured too much alcohol into the bag and tried to "wave" the bag and get rid of excess alcohol and a shower landed less than an inch from my eye. (again, very noob, i apologize for being a first timer) btw a spark definitely wouldn;t have helped haha
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